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Cited by 26Escherichia coli research studiesAntibiotic Resistance in BacteriaEnterobacteriaceae and Cronobacter Research

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Possible Dissemination of Escherichia coli Sequence Type 410 Closely Related to B4/H24RxC in Ghana

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Mahazu, Samiratu, Prah, Isaac, Ayibieke, Alafate, Sato, Wakana, Hayashi, Takaya, Suzuki, Toshihiko, Iwanaga, Shiroh, Ablordey, Anthony, Saito, Ryoichi·2021·Frontiers in Microbiology
DOI 10.3389/fmicb.2021.770130PMC PMC8672054
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Abstract

Extra-intestinal pathogenic Escherichia coli (ExPEC) is one of the world’s leading causes of bloodstream infections with high mortality. Sequence type 410 (ST410) is an emerging ExPEC clone resistant to a wide range of antibiotics. In this study, we investigated the epidemiology of 21 ST410 E. coli isolates from two Ghanaian hospitals. We also investigated the isolates within a global context to provide further insight into the dissemination of this highly pathogenic clone. A phylogenetic tree of the 21 isolate genomes, along with 102 others from global collection, was constructed representing the ensuing clades and sub-clades of the ST: A/H53, B2/H24R, B3/H24Rx, and B4/H24RxC. The carbapenem-resistant sub-clade B4/H24RxC is reported to have emerged in the early 2000s when ST410 acquired an IncX3 plasmid carrying a blaOXA–181 carbapenemase gene, and a second carbapenemase gene, blaNDM–5, on a conserved IncFII plasmid in 2014. We identified, in this study, one blaOXA–181–carrying isolate belonging to B4/H24RxC sub-lineage and one carrying blaNDM–1 belonging to sub-lineage B3/H24Rx. The blaOXA–181 gene was found on a 51kb IncX3 plasmid; pEc1079_3. The majority (12/21) of our Ghanaian isolates were clustered with international strains described by previous authors as closely related strains to B4/H24RxC. Six others were clustered among the ESBL-associated sub-lineage B3/H24Rx and three with the globally disseminated sub-lineage B4/H24RxC. The results show that this highly pathogenic clone is disseminated in Ghana and, given its ability to transmit between hosts, it poses a serious threat and should be monitored closely.

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Metadata

Authors
Mahazu, S., Prah, I., Ayibieke, A., Sato, W., Hayashi, T., Suzuki, T., Iwanaga, S., Ablordey, A., & Saito, R.
Affiliations
  • Tokyo Medical and Dental University (JP)
  • University of Ghana (GH)
Corresponding author
  • Ablordey, Anthony
  • Saito, Ryoichi
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2021-12-01
Volume
12
Pages
770130-770130
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2021.770130
PMC
PMC8672054
OpenAlex
W4200591276
Cited by
26
References
0
Citations by year
2026: 4 · 2025: 8 · 2024: 9 · 2022: 5
FWCI
2.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Japan Agency for Medical Research and Development (JP19fm0108010, 20wm0125007, 20wm0225004, 20wm0225013)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
  • Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
Keywords
extraintestinal pathogenic Escherichia coli, carbapenem resistance, blaOXA-181, IncX3 plasmid, IncFII plasmid, blaNDM-1, extended-spectrum beta-lactamase, bloodstream infection, phylogenetic analysis, clonal spread, Ghana

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