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Cited by 6Microbial Fuel Cells and BioremediationEnzyme-mediated dye degradationMicrobial bioremediation and biosurfactants

Enhanced Bioremediation Potential of Shewanella decolorationis RNA Polymerase Mutants and Evidence for Novel Azo Dye Biodegradation Pathways

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Cai, Xunchao, Zheng, Xin, Wang, Yicheng, Tian, Li, Mao, Yanping·2022·Frontiers in Microbiology
DOI 10.3389/fmicb.2022.843807PMC PMC8981235
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Abstract

Bioremediation has been considered as a promising method for recovering chemical polluted environments. Here Shewanella decolorationis strain Ni1-3 showed versatile abilities in bioremediation. To improve the bioremediation activity, RNA polymerase (RNAP) mutations of strain Ni1-3 were screened. Eleven mutants were obtained, of which mutant #40 showed enhanced Amaranth (AMR) degradation capacity, while mutant #21 showed defected capacity in AMR degradation but greatly enhanced capacity in cathodic metal leaching which is three to four times faster than that of the wild-type (WT) strain Ni1-3, suggesting that different pathways were involved in these two processes. Transcriptional profiling and gene co-expression networks between the mutants (i.e., #40 and #22) and the WT strain disclosed that the non-CymA-Mtr but cytochrome b- and flavin-oxidoreductase-dominated azo dye degradation pathways existed in S. decolorationis, which involved key proteins TorC, TorA, YceJ, YceI, Sye4, etc. Furthermore, the involvement of TorA was verified by trimethylamine N-oxide reduction and molybdenum enzyme inhibitory experiments. This study clearly demonstrates that RNAP mutations are effective to screen active microbial candidates in bioremediation. Meanwhile, by clarifying the novel gene co-expression network of extracellular electron transfer pathways, this study provides new insights in azo dye degradation and broadens the application of Shewanella spp. in bioremediation as well.

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  • Shewanella spp. can break down solid oxidized metals in used LIBs, such as Ni(IV), Co(IV), and Mn(IV).

    “It has been seen that Shewanella spp.can break down solid oxidized metals in used LIBs, such as Ni (IV), Co (IV), and Mn (IV).”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [49]

    The Results and Discussion report that S. decolorationis and its mutants bioleached Ni, Co, and Mn from spent lithium-ion battery cathode material. But the Introduction says reduction of the specifically listed Ni(IV), Co(IV), and Mn(IV) in those batteries *might* occur; the reported leaching does not establish that those oxidation states were reduced. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Cai et al. demonstrate that RNAP mutations effectively screen bioremediation candidates, clarify gene co-expression networks, and expand the application of Shewanella spp. in azo dye removal.

    “Furthermore, Cai et al. [49] demonstrate that RNAP mutations effectively screen bioremediation candidates, clarify gene co-expression networks, and expand the application of Shewanella spp. in azo dye removal.”

    From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [49]

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Cited by

Sorted by citations

Authors
Cai, X., Zheng, X., Wang, Y., Tian, L., & Mao, Y.
Affiliations
Shenzhen University (CN)
Corresponding author
Mao, Yanping
ORCID
Venue
Frontiers in Microbiology
Publisher
Frontiers Media SA
Published
2022-03-22
Volume
13
Pages
843807-843807
ISSN
1664-302X (ISSN-L)
DOI
10.3389/fmicb.2022.843807
PMC
PMC8981235
OpenAlex
W4220755714
Cited by
6
References
1
Citations by year
2026: 2 · 2025: 3 · 2024: 1
FWCI
0.30
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Microbiology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (41907214, 51708358)
  • Postdoctoral Research Foundation of China
Topics
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Enzyme-mediated dye degradation
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
Keywords
azo dye biodegradation, extracellular electron transfer, gene co-expression network, TORA, bioremediation, gene expression profiling

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