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New β-Propellers Are Continuously Amplified From Single Blades in all Major Lineages of the β-Propeller Superfamily

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Pereira, Joana, Lupas, Andrei N.·2022·Frontiers in Molecular Biosciences
DOI 10.3389/fmolb.2022.895496PMC PMC9218822

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Abstract

β-Propellers are toroidal folds, in which consecutive supersecondary structure units of four anti-parallel β-strands–called blades–are arranged radially around a central axis. Uniquely among toroidal folds, blades span the full range of sequence symmetry, from near identity to complete divergence, indicating an ongoing process of amplification and differentiation. We have proposed that the major lineages of β-propellers arose through this mechanism and that therefore their last common ancestor was a single blade, not a fully formed β-propeller. Here we show that this process of amplification and differentiation is also widespread within individual lineages, yielding β-propellers with blades of more than 60% pairwise sequence identity in most major β-propeller families. In some cases, the blades are nearly identical, indicating a very recent amplification event, but even in cases where such recently amplified β-propellers have more than 80% overall sequence identity to each other, comparison of their DNA sequence shows that the amplification occurred independently.

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Metadata

Authors
Pereira, J., & Lupas, A. N.
Affiliations
Max Planck Institute for Biology (DE)
Corresponding author
  • Pereira, Joana
  • Lupas, Andrei N.
ORCID
Venue
Frontiers in Molecular Biosciences
Publisher
Frontiers Media SA
Published
2022-06-09
Volume
9
Pages
895496-895496
ISSN
2296-889X (ISSN-L)
DOI
10.3389/fmolb.2022.895496
PMC
PMC9218822
OpenAlex
W4281991817
Cited by
8
References
2
Citations by year
2026: 2 · 2025: 2 · 2024: 4
FWCI
0.49
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Molecular Biosciences (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Max-Planck-Gesellschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
beta-propeller, protein evolution, sequence divergence, supersecondary structure, common ancestor, DNA sequence comparison

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