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Cited by 15Genetic and Kidney Cyst DiseasesDermatology and Skin DiseasesContact Dermatitis and Allergies

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Dendritic cell proliferation by primary cilium in atopic dermatitis

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Toriyama, Manami, Rizaldy, Defri, Nakamura, Motoki, Atsumi, Yukiko, Toriyama, Michinori, Fujita, Fumitaka, Okada, Fumihiro, Morita, Akimichi, Itoh, Hiroshi, Ishii, Ken J.·2023·Frontiers in Molecular Biosciences
DOI 10.3389/fmolb.2023.1149828PMC PMC10169737
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Abstract

Introduction: Atopic dermatitis (AD) is a common allergic eczema that affects up to 10% of adults in developed countries. Immune cells in the epidermis, namely, Langerhans cells (LCs), contribute to the pathogenesis of AD, although their exact role(s) in disease remain unclear. Methods: We performed immunostaining on human skin and peripheral blood mononuclear cells (PBMCs) and visualized primary cilium. Result and discussion: We show that human dendritic cells (DCs) and LCs have a previously unknown primary cilium-like structure. The primary cilium was assembled during DC proliferation in response to the Th2 cytokine GM-CSF, and its formation was halted by DC maturation agents. This suggests that the role of primary cilium is to transduce proliferation signaling. The platelet-derived growth factor receptor alpha (PDGFRα) pathway, which is known for transducing proliferation signals in the primary cilium, promoted DC proliferation in a manner dependent on the intraflagellar transport (IFT) system. We also examined the epidermal samples from AD patients, and observed aberrantly ciliated LCs and keratinocytes in immature and proliferating states. Our results identify a potential relationship between the primary cilium and allergic skin barrier disorders, and suggest that targeting the primary cilium may contribute to treating AD.

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Metadata

Authors
Toriyama, M., Rizaldy, D., Nakamura, M., Atsumi, Y., Toriyama, M., Fujita, F., Okada, F., Morita, A., Itoh, H., & Ishii, K. J.
Affiliations
  • Kwansei Gakuin University (JP)
  • Bandung Institute of Technology (ID)
  • Nagoya City University (JP)
  • National Institute of Biomedical Innovation, Health and Nutrition (JP)
  • Mandom Corp. (Japan) (JP)
  • Nara Institute of Science and Technology (JP)
Corresponding author
  • Toriyama, Manami
  • Ishii, Ken J.
ORCID
Venue
Frontiers in Molecular Biosciences
Publisher
Frontiers Media SA
Published
2023-04-26
Volume
10
Pages
1149828-1149828
ISSN
2296-889X (ISSN-L)
DOI
10.3389/fmolb.2023.1149828
PMC
PMC10169737
OpenAlex
W4367053207
Cited by
15
References
2
Citations by year
2026: 3 · 2025: 4 · 2024: 4 · 2023: 4
FWCI
2.49
Type
journal article
Language
en
Update notices
corrigendum (2023-08-30)
Open access
Licence
Locations
  • Frontiers in Molecular Biosciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Japan Society for the Promotion of Science (JP19K17797)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
Keywords
atopic dermatitis, primary cilia, dendritic cell proliferation, Langerhans cells, GM-CSF, PDGFRα signaling, intraflagellar transport, keratinocytes, ciliopathies

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