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Automated assessment of Pavlovian conditioned freezing and shock reactivity in mice using the VideoFreeze system

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Anagnostaras, Stephan G.·2010·Frontiers in Behavioral Neuroscience
DOI 10.3389/fnbeh.2010.00158PMC PMC2955491
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Abstract

The Pavlovian conditioned freezing paradigm has become a prominent mouse and rat model of learning and memory, as well as of pathological fear. Due to its efficiency, reproducibility and well-defined neurobiology, the paradigm has become widely adopted in large-scale genetic and pharmacological screens. However, one major shortcoming of the use of freezing behavior has been that it has required the use of tedious hand scoring, or a variety of proprietary automated methods that are often poorly validated or difficult to obtain and implement. Here we report an extensive validation of the Video Freeze system in mice, a "turn-key" all-inclusive system for fear conditioning in small animals. Using digital video and near-infrared lighting, the system achieved outstanding performance in scoring both freezing and movement. Given the large-scale adoption of the conditioned freezing paradigm, we encourage similar validation of other automated systems for scoring freezing, or other behaviors.

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  • Active avoidance tests are particularly valuable for evaluating hippocampal-dependent cognitive functions.

    “Active avoidance tests are particularly valuable for evaluating hippocampal-dependent cognitive functions (Anagnostaras et al., 2010; Chaudry et al., 2022; Kajs et al., 2022).”

    From Arbovirus infection increases the risk for the development of neurodegenerative disease pathology in the murine model (2024) · cites this paper as Anagnostaras et al., 2010

    The Introduction (page unavailable in our copy) describes contextual fear conditioning as hippocampus-dependent and Pavlovian fear conditioning as a useful memory assay. It does not establish that active avoidance tests are particularly valuable for evaluating hippocampal-dependent cognitive functions; that is a different test paradigm. (LLM, conf=0.82)

    Experimental — accuracy not yet measured

  • Previous studies demonstrate that in animal models of neurodegenerative disorders, such as AD, cognitive function and learning and memory are significantly impaired.

    “Previous studies demonstrate that in animal models of neurodegenerative disorders, such as AD, cognitive function and learning and memory are significantly impaired (Anagnostaras et al., 2010; Chaudry et al., 2022; Kajs et al., 2022; Webster et al., 2014) and that VEEV infections can have an adverse effect on learning memory (Dave and Klein, 2023; Ronca et al., 2016).”

    From Arbovirus infection increases the risk for the development of neurodegenerative disease pathology in the murine model (2024) · cites this paper as Anagnostaras et al., 2010

    The Introduction (page unavailable) describes fear conditioning as a memory assay and mentions Alzheimer's disease as a potential target for cognitive-function research. It does not report significant cognitive or learning-and-memory impairment in animal models of Alzheimer's or other neurodegenerative disorders; searches for those models and impairments found no supporting passage. (LLM, conf=0.76)

    Experimental — accuracy not yet measured

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Authors
Anagnostaras, S. G.
Affiliations
University of California San Diego (US)
Corresponding author
Anagnostaras, Stephan G.
ORCID
Anagnostaras, Stephan G.
Venue
Frontiers in Behavioral Neuroscience
Publisher
Frontiers Media SA
Published
2010
Volume
4
ISSN
1662-5153 (ISSN-L)
DOI
10.3389/fnbeh.2010.00158
PMC
PMC2955491
OpenAlex
W2146054031
Cited by
169
References
93
Citations by year
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FWCI
5.63
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Behavioral Neuroscience (publishedVersion, cc-by)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Science Foundation
  • National Institutes of Health
  • National Institute on Drug Abuse (F31DA026259)
Topics
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
fear conditioning, motion detection, behavioral validation, learning and memory

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