The mode of sucrose degradation in potato tubers determines the fate of assimilate utilization

Ferreira, Stephanus J.; Sonnewald, Uwe

doi:10.3389/fpls.2012.00023

Cited by 39Plant nutrient uptake and metabolismPostharvest Quality and Shelf Life ManagementPotato Plant Research

The mode of sucrose degradation in potato tubers determines the fate of assimilate utilization

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Ferreira, Stephanus J., Sonnewald, Uwe·2012·Frontiers in Plant Science

DOI 10.3389/fpls.2012.00023PMC PMC3355675

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Abstract

Cytosolic (U-IN-2) or apoplasmic (U-IN-1) targeting of yeast invertase in potato tubers leads to a reduction in sucrose and an increase in glucose content, but specific phenotypical changes are dependent on the subcellular targeting of the enzyme. Cytosolic expression leads to a more severe phenotype with the most striking aspects being reduced starch content and increased respiration. Despite extensive research, the regulatory mechanisms leading to these changes remain obscure. Recent technological advancements regarding potato transcriptional and genomic research presented us with the opportunity to revisit these lines and perform detailed gene expression analysis, in combination with extensive metabolic profiling, to identify regulatory networks underlying the observed changes. Our results indicate that in both genotypes reduced UDP-glucose production is associated with a reduced expression of cell wall biosynthetic genes. In addition, U-IN-1 tubers are characterized by elevated expression of senescence-associated genes, coupled to reduced expression of genes related to photosynthesis and the cytoskeleton. We provide evidence that increased respiration, observed specifically in U-IN-2 tubers, might be due to sugar signaling via released trehalose-6-phosphate inhibition of the SnRK1 complex. In both genotypes, expression of the plastidic glucose-6-phosphate transporter (GPT) is significantly down-regulated. This leads to a shift in the cytosolic to plastidic glucose-6-phosphate ratio and hence might limit starch synthesis but also the oxidative pentose phosphate pathway. This might explain the observed changes in several additional plastid localized pathways, most notably reduced expression of fatty acid biosynthetic genes and an accumulation of shikimate. Interestingly, a strict negative correlation between invertase and GPT expression could be observed in a wide range of potato tubers. This reciprocal regulation may be part of a more general switch controlling energy versus storage metabolism, suggesting that the fate of assimilate utilization is coordinated at the level of sucrose degradation.

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SPS, SPP, PGI, SuSy, and UGPase all directly or indirectly participate in starch biosynthesis through interconverting sugar and UDP-glucose.
“SPS, SPP, PGI, SuSy, and UGPase all directly or indirectly participate in starch biosynthesis through interconverting sugar and UDP-glucose (Table S8 and Figure S2, Ferreira and Sonnewald, 2012).”
From Comparative Transcriptome Analysis Reveals Critical Function of Sucrose Metabolism Related-Enzymes in Starch Accumulation in the Storage Root of Sweet Potato (2017) · cites this paper as Ferreira and Sonnewald, 2012
The Results and Discussion section on starch biosynthetic capacity describes SuSy, PGI, and UGPase as part of routes that supply glucose-6-phosphate for starch biosynthesis. Our copy does not establish the attributed roles of SPS or SPP, so it does not support the claim for all five enzymes. The supplied passages have no page numbers. (LLM, conf=0.86)
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Authors: Ferreira, S. J., & Sonnewald, U.
Affiliations: Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
Corresponding author: Sonnewald, Uwe
ORCID: Sonnewald, Uwe
Venue: Frontiers in Plant Science
Publisher: Frontiers Media SA
Published: 2012
Volume: 3
Pages: 23-23
ISSN: 1664-462X (ISSN-L)
DOI: 10.3389/fpls.2012.00023
PMC: PMC3355675
OpenAlex: W2131158448
Cited by: 39
References: 76
Citations by year: 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 6 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 1
FWCI: 1.83
Type: journal article
Language: en
Open access: gold
doi.org
Licence: cc-by-nc
Locations: Frontiers in Plant Science (publishedVersion, cc-by-nc)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
OPUS Repository (Kooperativer Bibliotheksverbund Berlin-Brandenburg) (submittedVersion, cc-by-nc)
Funders: Bundesministerium für Bildung und Forschung
Topics: Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
Keywords: sucrose degradation, potato tubers, yeast invertase, subcellular targeting, UDP-glucose, starch biosynthesis, respiration, SnRK1, glucose-6-phosphate transporter, oxidative pentose phosphate pathway, senescence-associated genes, cell wall biosynthesis, shikimate accumulation, gene expression profiling, metabolite profiling

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The mode of sucrose degradation in potato tubers determines the fate of assimilate utilization

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