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Cited by 82Powdery Mildew Fungal DiseasesPlant-Microbe Interactions and ImmunityYeasts and Rust Fungi Studies

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Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action

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Gafni, Aviva, Calderon, Claudia E., Harris, Raviv, Buxdorf, Kobi, Dafa-Berger, Avis, Zeilinger-Reichert, Einat, Levy, Maggie·2015·Frontiers in Plant Science
DOI 10.3389/fpls.2015.00132PMC PMC4356082
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Abstract

Epiphytic yeasts, which colonize plant surfaces, may possess activity that can be harnessed to help plants defend themselves against various pathogens. Due to their unique characteristics, epiphytic yeasts belonging to the genus Pseudozyma hold great potential for use as biocontrol agents. We identified a unique, biologically active isolate of the epiphytic yeast Pseudozyma aphidis that is capable of inhibiting Botrytis cinerea via a dual mode of action, namely induced resistance and antibiosis. Here, we show that strain L12 of P. aphidis can reduce the severity of powdery mildew caused by Podosphaera xanthii on cucumber plants with an efficacy of 75%. Confocal and scanning electron microscopy analyses demonstrated P. aphidis proliferation on infected tissue and its production of long hyphae that parasitize the powdery mildew hyphae and spores as an ectoparasite. We also show that crude extract of P. aphidis metabolites can inhibit P. xanthii spore germination in planta. Our results suggest that in addition to its antibiosis as mode of action, P. aphidis may also act as an ectoparasite on P. xanthii. These results indicate that P. aphidis strain L12 has the potential to control powdery mildew.

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  • Pseudozyma aphidis parasitises the powdery mildew pathogen Podosphaera xanthii and B. cinerea.

    “the yeast-like Ustilaginomycete Pseudozyma aphidis parasitises the powdery mildew pathogen Podosphaera xanthii and B. cinerea (Calderon et al. 2019; Gafni et al. 2015).”

    From Biocontrol yeasts: mechanisms and applications (2019) · cites this paper as Gafni et al. 2015

    The Discussion says that P. aphidis coiled around P. xanthii hyphae, suggesting ectoparasitism. The Introduction attributes its activity against B. cinerea to antibiosis and induced resistance, not parasitism; the paper therefore supports the claim for P. xanthii but not for B. cinerea. (LLM, conf=0.90)

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Metadata

Authors
Gafni, A., Calderon, C. E., Harris, R., Buxdorf, K., Dafa-Berger, A., Zeilinger-Reichert, E., & Levy, M.
Affiliations
Hebrew University of Jerusalem (IL)
Corresponding author
Levy, Maggie
ORCID
Levy, Maggie
Venue
Frontiers in Plant Science
Publisher
Frontiers Media SA
Published
2015-03-11
Volume
6
Pages
132-132
ISSN
1664-462X (ISSN-L)
DOI
10.3389/fpls.2015.00132
PMC
PMC4356082
OpenAlex
W2047469625
Cited by
82
References
1
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 14 · 2022: 6 · 2021: 14 · 2020: 10 · 2019: 7 · 2018: 4 · 2017: 13
FWCI
6.86
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Frontiers in Plant Science (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Powdery Mildew Fungal Diseases
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Immunology and Microbiology › Microbiology › Yeasts and Rust Fungi Studies
Keywords
biological control, Podosphaera xanthii, powdery mildew, Pseudozyma aphidis, epiphytic yeasts, ectoparasite infestation, induced resistance, antibiosis, Botrytis cinerea, cucumber, spore germination inhibition

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