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Tobacco BY-2 Media Component Optimization for a Cost-Efficient Recombinant Protein Production

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Häkkinen, Suvi T., Reuter, Lauri, Nuorti, Ninni, Joensuu, Jussi J., Rischer, Heiko, Ritala, Anneli·2018·Frontiers in Plant Science
DOI 10.3389/fpls.2018.00045PMC PMC5791008
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Abstract

Plant cells constitute an attractive platform for production of recombinant proteins as more and more animal-free products and processes are desired. One of the challenges in using plant cells as production hosts has been the costs deriving from expensive culture medium components. In this work, the aim was to optimize the levels of most expensive components in the nutrient medium without compromising the accumulation of biomass and recombinant protein yields. Wild-type BY-2 culture and transgenic tobacco BY-2 expressing green fluorescent protein-Hydrophobin I (GFP-HFBI) fusion protein were used to determine the most inexpensive medium composition. One particularly high-accumulating BY-2 clone, named 'Hulk,' produced 1.1 ± 0.2 g/l GFP-HFBI in suspension and kept its high performance during prolonged subculturing. In addition, both cultures were successfully cryopreserved enabling truly industrial application of this plant cell host. With the optimized culture medium, 43-55% cost reduction with regard to biomass and up to 69% reduction with regard to recombinant protein production was achieved.

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  • Plant cell suspensions like BY-2 frequently exhibit somaclonal variation, impacting secondary metabolite profiles and reducing productivity.

    “In agricultural biotechnology, plant cell suspensions like the tobacco Bright Yellow-2 (BY-2) frequently exhibit somaclonal variation, impacting secondary metabolite profiles and reducing productivity [82].”

    From Genetic Insights into the Economic Toll of Cell Line Misidentification: A Comprehensive Review (2026) · cites this paper as [82]

    The Introduction (page unavailable) says plant cell cultures often exhibit somaclonal variation that can lead to loss of productivity or metabolic competence. It does not say that the variation changes secondary metabolite profiles, and the Discussion (page unavailable) reports stable recombinant-protein expression in the BY-2 cells studied rather than a productivity decline in those cells. (LLM, conf=0.86)

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Research works printed but not linked: 25

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Cited by

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Authors
Häkkinen, S. T., Reuter, L., Nuorti, N., Joensuu, J. J., Rischer, H., & Ritala, A.
Affiliations
VTT Technical Research Centre of Finland (FI)
Corresponding author
Häkkinen, Suvi T.
ORCID
Venue
Frontiers in Plant Science
Publisher
Frontiers Media SA
Published
2018-01-26
Volume
9
Pages
45-45
ISSN
1664-462X (ISSN-L)
DOI
10.3389/fpls.2018.00045
PMC
PMC5791008
OpenAlex
W2793825735
Cited by
50
References
0
Citations by year
2026: 5 · 2025: 6 · 2024: 7 · 2023: 8 · 2022: 8 · 2021: 7 · 2020: 5 · 2019: 2 · 2018: 2
FWCI
2.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Plant Science (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
Academy of Finland (252442)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
Keywords
tobacco BY-2 cells, culture medium optimization, recombinant protein expression, cost reduction, hydrophobins, transgenic tobacco, suspension culture, cryopreservation, plant cell bioreactors, biomass accumulation

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