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Cited by 42Seed Germination and PhysiologyPlant Stress Responses and ToleranceLipid metabolism and biosynthesis

How does the seed pre-germinative metabolism fight against imbibition damage? Emerging roles of fatty acid cohort and antioxidant defence.

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Doria, Enrico, Pagano, Andrea, Ferreri, Carla, Larocca, Anna Vita, Macovei, Anca, Araújo, Susana de Sousa, Balestrazzi, Alma·2019·Frontiers in Plant Science
DOI 10.3389/fpls.2019.01505PMC PMC6881478
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Abstract

During seed imbibition, lipids are engaged in membrane reorganization while facing free radical-mediated oxidative injury. In the present work, we explored changes in lipid components at different timepoints of imbibition (0.5, 2, 4, 6, and 8 h) in the legume Medicago truncatula, by combining biochemical approaches with targeted lipidomics and untargeted metabolomics. ROS and RNS (reactive oxygen and nitrogen species) accumulation was observed throughout the tested timepoints whereas lipid peroxidation increased at 4 h of imbibition. The seed response to oxidative damage was evidenced by a significant increase in tocopherols starting from 0.5 h of imbibition as well as by the reduction in total thiol content occurring at 2 h of imbibition. Since under physiological conditions, the proper functions of the cell membranes are strongly dependent on the qualitative and quantitative balance of fatty acid residues in phospholipids, the investigation was expanded to the fatty acid cohort of Medicago truncatula seeds. Total saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), omega-3 and omega-6 fatty acids showed fluctuations during seed imbibition. The most remarkable finding was the profile of the omega-3 PUFA docosopentaenoic acid (DPA, 7 cis, 10 cis, 13 cis, 16 cis, 19 cis-22:5) that showed a peak (up to 1.0% of the total fatty acid content) at 0.5 and 8 h of imbibition, respectively, concomitant with the peaks observed in tocopherol levels. It is possible that the observed changes in DPA alter the physical properties of membranes, as reported in animal cells, triggering signaling pathways relevant for the cell defence against oxidative injury. Furthermore, the content and balance between tocopherols and PUFAs is regarded as a determinant of storage stability. No enhancement in trans-fatty acids occurred throughout imbibition, suggesting for a proper antioxidant response carried by the seed. Fatty acids profiles were integrated with data from untargeted metabolomics showing changes in lipid sub-pathways, among which fatty acid amide, lyso-phospholipids and phospholipid metabolism. The emerging lipid profiles and dynamics are discussed in view of the overall imbibition damage generated during M. truncatula seed imbibition.

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Authors
Doria, E., Pagano, A., Ferreri, C., Larocca, A. V., Macovei, A., Araújo, S. D. S., & Balestrazzi, A.
Affiliations
  • University of Pavia (IT)
  • Bologna Research Area (IT)
  • Instituto de Tecnologia Química e Biológica António Xavier (PT)
Corresponding author
Balestrazzi, Alma
ORCID
Venue
Frontiers in Plant Science
Publisher
Frontiers Media SA
Published
2019-11-21
Volume
10
Pages
1505-1505
ISSN
1664-462X (ISSN-L)
DOI
10.3389/fpls.2019.01505
PMC
PMC6881478
OpenAlex
W2989698631
Cited by
42
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0
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2026: 10 · 2025: 8 · 2024: 3 · 2023: 10 · 2022: 8 · 2021: 1 · 2020: 2
FWCI
2.03
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Plant Science (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Archivio istituzionale della ricerca (Alma Mater Studiorum Università di Bologna) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Fondazione Cariplo
  • Ministero dell’Istruzione, dell’Università e della Ricerca
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Seed Germination and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
seed imbibition, Medicago truncatula, seed germination, lipid peroxidation, reactive oxygen species, reactive nitrogen species, tocopherols, thiol content, docosapentaenoic acid, omega-3 polyunsaturated fatty acids, fatty acid composition, phospholipid metabolism, fatty acid amides, lysophospholipids, antioxidant defense

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