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Cited by 60Escherichia coli research studiesSalmonella and Campylobacter epidemiologyAntibiotic Resistance in Bacteria

Diarrheagenic Escherichia coli Pathotypes From Children Younger Than 5 Years in Kano State, Nigeria

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Saka, Habeeb Kayode, Dabo, Nasir Tukur, Muhammad, Bashir, García-Soto, Silvia, Ugarte-Ruiz, Maria, Alvarez, Julio·2019·Frontiers in Public Health
DOI 10.3389/fpubh.2019.00348PMC PMC6890574
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Abstract

Diarrhoeagenic Escherichia coli (DEC) is one of the leading cause of gastrointestinal disorders worldwide and an important public health challenge. It is often underdiagnosed during routine microbiological analysis, especially in resource constrained settings. The use of molecular tests could however help to determine the distribution of DEC and their clinical significance. Here, a study to assess the prevalence of DEC in clinical samples from patients <5 years attending three hospitals in Kano state, Nigeria was carried out. Samples from 400 patients and 50 controls were collected and screened for E. coli. Compatible colonies from 248 individuals (215 patients and 33 controls) were characterized using biochemical test, real-time PCR for detection of nine virulence factors (VF: eae, bfpA, elt, est, stx1, stx2, ehxA, aggR and invA) associated with five DEC pathotypes (EPEC, ETEC, EHEC, EAEC and EIEC) and antimicrobial susceptibility tests. One or more VFs typical to a pathotype were detected in 73.8% (183/248) of the isolates, with those associated with EAEC(36.3%), ETEC(17.3%) and EPEC(6.0%) being the most common, although proportion of specific pathotypes differ between hospitals. est was the only VF detected in a significantly higher proportion in cases compared to controls (P-value=0.034). Up to 86.9% of DEC were resistant to at least one class of antibiotics, with trimethoprim-sulfamethoxazole being the least effective drug (77.6% resistance). Our results demonstrate widespread circulation of different DEC pathotypes among children in Kano state that were highly resistant to trimethoprim-sulfamethoxazole, and highlight the need of characterizing the causative agents in cases of gastrointestinal disorders.

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  • In sub-Saharan Africa, the precise burden associated with diarrheagenic Escherichia coli is not known; however, prevalence rates ranging from 14.7% to 82% have been reported in Nigeria, Gabon, Burkina Faso, Kenya, South Africa, and Tanzania.

    “In SSA, the precise burden associated with diarrheagenic E. coli is not known; however, prevalence rates ranging from 14.7% to 82% have been reported in Nigeria, 37 Gabon, 38 Burkina Faso, 39 Kenya, 40 South Africa, 41 and Tanzania.42”

    From Antimicrobial Resistance Rates and Surveillance in Sub-Saharan Africa: Where Are We Now? (2022) · cites this paper as [37]

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Authors
Saka, H. K., Dabo, N. T., Muhammad, B., García-Soto, S., Ugarte-Ruiz, M., & Alvarez, J.
Affiliations
  • Nigerian Stored Products Research Institute (NG)
  • Bayero University Kano (NG)
  • Universidad Complutense de Madrid (ES)
Corresponding author
Saka, Habeeb Kayode
ORCID
Venue
Frontiers in Public Health
Publisher
Frontiers Media SA
Published
2019-11-27
Volume
7
Pages
348-348
ISSN
2296-2565 (ISSN-L)
DOI
10.3389/fpubh.2019.00348
PMC
PMC6890574
OpenAlex
W2990000774
Cited by
60
References
1
Citations by year
2026: 5 · 2025: 7 · 2024: 8 · 2023: 11 · 2022: 20 · 2021: 6 · 2020: 3
FWCI
4.74
Type
journal article
Language
en
Open access
Licence
Locations
  • Frontiers in Public Health (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Keywords
diarrheagenic Escherichia coli, Escherichia coli pathotypes, childhood diarrhea, virulence factors, enteropathogenic Escherichia coli, enterotoxigenic Escherichia coli, enterohemorrhagic Escherichia coli, enteroinvasive Escherichia coli, antimicrobial resistance, trimethoprim-sulfamethoxazole resistance, stx1, stx2, Kano State

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