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Cited by 25Medicinal Plant ResearchPhytochemicals and Antioxidant ActivitiesPhytochemical Studies and Bioactivities

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Biopotential of Bersama abyssinica Fresen Stem Bark Extracts: UHPLC Profiles, Antioxidant, Enzyme Inhibitory, and Antiproliferative Propensities

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Sinan, Kouadio Ibrahime, Chiavaroli, Annalisa, Orlando, Giustino, Bene, Kouadio, Zengin, Gokhan, Cziáky, Zoltán, Jekő, József, Fawzi Mahomoodally, Mohamad, Picot-Allain, Marie Carene Nancy, Menghini, Luigi, Recinella, Lucia, Brunetti, Luigi, Leone, Sheila, Ciferri, Maria Chiara, Di Simone, Simonetta, Ferrante, Claudio·2020·Antioxidants
DOI 10.3390/antiox9020163PMC PMC7094211
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Abstract

In this study, ethyl acetate, methanol, and water extracts of Bersama abyssinica (Melianthaceae) stem bark were screened for enzyme inhibitory and antioxidant properties. The water extract possessed the highest concentration of phenols (230.83 mg gallic acid equivalent/g extract), while the methanol extract was rich in flavonoids (75.82 mg rutin equivalent/g extract), and the ethyl acetate extract possessed the highest amount of saponins (97.37 mg quillaja equivalent/g). The aim of this study was to investigate the antiproliferative effects against the human colon cancer HCT116 cell line challenged with serotonin (5-HT) as a stimulating-proliferation factor. The level of HCT116 cell-deriving pool of kynurenic acid (KA) was also assessed. The UHPLC results confirmed the presence of 58, 68, and 63 compounds in the ethyl acetate, methanol, and water extracts, respectively. Mangiferin, vitexin and its isomer isovitexin were tentatively identified in all extracts and KA (m/z 190.05042 [M−H]+) was also tentatively identified in the methanol and water extracts. The methanol extract (1464.08 mg Trolox equivalent [TE]/g extract) showed the highest activity in the CUPRAC assay, whereas the water extract (1063.70 mg TE/g extract) showed the highest activity with the FRAP technique. The ethyl acetate extract was the most active acetylcholinesterase (4.43 mg galantamine equivalent/g extract) and α-glucosidase (mmol acarbose equivalent /g extract) inhibitor. The water extract was able to inhibit 5-HT-stimulated viability of HCT116 cells, and blunt 5-HT-induced reduction of cell-deriving KA. The scientific data generated in this study provide baseline data regarding the biological properties of B. abyssinica stem bark, highlighting its potential use for the development of new pharmaceutic and cosmetic agents.

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  • The FRAP assay is a well-known, reliable approach that measures ferric reducing ability in a redox-linked colorimetric reaction that reduces Fe3+ to Fe2+.

    “The FRAP assay is a well-known, reliable approach that measures ferric reducing ability in a redox-linked colorimetric reaction that reduces Fe3+ to Fe2+ [78–81].”

    From Comparative and Correlational Evaluation of the Phytochemical Constituents and Antioxidant Activity of Musa sinensis L. and Musa paradisiaca L. Fruit Compartments (Musaceae) (2020) · cites this paper as [81]

    Section 3.1 says the authors used FRAP to determine the extracts’ reducing potential, supporting that part of the claim. Section 2.3 refers readers to an earlier paper for method details; our copy does not describe the Fe3+-to-Fe2+ colorimetric reaction or assess FRAP’s reliability. No page numbers are available in the indexed passages. (LLM, conf=0.86)

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Research works printed but not linked: 31

  1. Amit, 2010, Phytochemistry and pharmacological activities of Bersama engleriana Guerke-An overview, Sex. Transm. Dis., 11, 12
  2. Kidane, 2014, Use and management of traditional medicinal plants by Maale and Ari ethnic communities in southern Ethiopia, J. Ethnobiol. Ethnomed., 10, 46, 10.1186/1746-4269-10-46 · DOI
  3. Zekeya, 2014, Analysis of phytochemical composition of Bersama abyssinica by gas chromatography-mass spectrometry, J. Pharmacogn. Phytochem., 3, 246
  4. Tuasha, 2018, Medicinal plants used by traditional healers to treat malignancies and other human ailments in Dalle District, Sidama Zone, Ethiopia, J. Ethnobiol. Ethnomedicine, 14, 15, 10.1186/s13002-018-0213-z · DOI
  5. Kupchan, 1971, Tumor inhibitors. LXIV. Isolation and structural elucidation of novel bufadienolides, the cytotoxic principles of Bersama abyssinica, Bioorg. Chem., 1, 13, 10.1016/0045-2068(71)90003-4 · DOI
  6. Kupchan, 1968, The isolation and characterization of hellebrigenin 3-acetate and hellebrigenin 3,5-diacetate, bufadienolide tumor inhibitors from Bersama abyssinica, Tetrahedron Lett., 9, 149, 10.1016/S0040-4039(00)75577-8 · DOI
  7. Ameya, 2019, Phytochemical Analysis and Antimicrobial Activity of Bersama abyssinica Fresen against Multidrug-Resistant Bacterial Uropathogens: Picolinyl Hydrazide Is a Major Compound, J. HerbsSpices Med. Plants, 25, 389
  8. Ataee, 2010, Study of 5HT3 and HT4 receptor expression in HT29 cell line and human colon adenocarcinoma tissues, Arch. Iran. Med., 13, 120
  9. Ballou, 2018, 5-HT serotonin receptors modulate mitogenic signaling and impact tumor cell viability, Mol. Clin. Oncol., 9, 243
  10. Regmi, 2014, Serotonin regulates innate immune responses of colon epithelial cells through Nox2-derived reactive oxygen species, Free Radic. Biol. Med., 69, 377, 10.1016/j.freeradbiomed.2014.02.003 · DOI
  11. Uysal, 2015, A phytochemical study on Potentilla anatolica: An endemic Turkish plant, Ind. Crop. Prod., 76, 1001, 10.1016/j.indcrop.2015.08.017 · DOI
  12. Blazekovic, 2011, Antioxidant activities and polyphenolic contents of three selected Micromeria species from Croatia, Molecules, 16, 1454, 10.3390/molecules16021454 · DOI · Paper page
  13. Zengin, 2014, Investigation of antioxidant potentials of solvent extracts from different anatomical parts of Asphodeline anatolica E. Tuzlaci: An endemic plant to Turkey, Afr. J. Tradit. Complement. Altern. Med., 11, 481, 10.4314/ajtcam.v11i2.37 · DOI
  14. Djemgou, 2010, C-Glucoside xanthone from the stem bark extract of Bersama engleriana, Pharmacogn. Res., 2, 229, 10.4103/0974-8490.69110 · DOI
  15. Kupchan, 1971, Tumor inhibitors. LXV. Bersenogenin, berscillogenin, and 3-epiberscillogenin, three new cytotoxic bufadienolides from Bersama abyssinica, J. Org. Chem., 36, 2611, 10.1021/jo00817a011 · DOI
  16. Di Giacomo, V., Chiavaroli, C., Orlando, G., Cataldi, A., Rapino, M., Di Valerio, V., Leone, S., Brunetti, L., Menghini, L., and Recinella, L. (2020). Neuroprotective and Neuromodulatory Effects Induced by Cannabidiol and Cannabigerol in Rat Hypo-E22 cells and Isolated Hypothalamus. Antioxidants, 9. · DOI
  17. Aktumsek, 2013, Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species, Food Chem. Toxicol., 55, 290, 10.1016/j.fct.2013.01.018 · DOI
  18. Grochowski, 2017, In vitro enzyme inhibitory properties, antioxidant activities, and phytochemical profile of Potentilla thuringiaca, Phytochem. Lett., 20, 365, 10.1016/j.phytol.2017.03.005 · DOI
  19. Badal, S., and Delgoda, R. (2017). Chapter 7—Evolutionary Perspectives on the Role of Plant Secondary Metabolites. Pharmacognosy, Academic Press. · DOI
  20. Sinan, K.I., Bene, K., Zengin, G., Diuzheva, A., Jekő, J., Cziáky, Z., Picot-Allain, C.M.N., Mollica, A., Rengasamy, K.R., and Mahomoodally, M.F. (2019). A comparative study of the HPLC-MS profiles and biological efficiency of different solvent leaf extracts of two African plants: Bersama abyssinica and Scoparia dulcis. Int. J. Environ. Health Res., 1–13. · DOI
  21. Pineda, 2018, Relevance of Alternative Routes of Kynurenic Acid Production in the Brain, Oxidative Med. Cell. Longev., 2018, 14
  22. Blanco, 2017, Chapter 8—Enzymes, Medical Biochemistry, Volume 8, 153
  23. Curtis, 2018, Cell Line-Specific Direct Irradiation and Bystander Responses are Influenced by Fetal Bovine Serum Serotonin Concentrations, Radiat. Res., 190, 262, 10.1667/RR15072.1 · DOI
  24. Tsai, 2011, Tazarotene-induced gene 1 inhibits prostaglandin E2-stimulated HCT116 colon cancer cell growth, J. Biomed. Sci., 18, 88, 10.1186/1423-0127-18-88 · DOI
  25. Notarangelo, 2019, Assessment of Prenatal Kynurenine Metabolism Using Tissue Slices: Focus on the Neosynthesis of Kynurenic Acid in Mice, Dev. Neurosci., 41, 1, 10.1159/000499736 · DOI
  26. Fukui, 1991, Blood–brain barrier transport of kynurenines: Implications for brain synthesis and metabolism, J. Neurochem., 56, 2007, 10.1111/j.1471-4159.1991.tb03460.x · DOI
  27. Ferrante, 2017, Protective Effects Induced by Microwave-Assisted Aqueous Harpagophytum Extract on Rat Cortex Synaptosomes Challenged with Amyloid β-Peptide, Phytother. Res., 31, 1257, 10.1002/ptr.5850 · DOI
  28. Czinege, 2013, Unexpected effects of peripherally administered kynurenic acid on cortical spreading depression and related blood–brain barrier permeability, Drug Des. Dev. Ther., 7, 981
  29. Marciniak, 2018, Kynurenic Acid Protects against Thioacetamide-Induced Liver Injury in Rats, Anal. Cell. Pathol., 2018, 1270483, 10.1155/2018/1270483 · DOI
  30. Walczak, 2014, Kynurenic acid inhibits colon cancer proliferation in vitro: Effects on signaling pathways, Amino Acids, 46, 2393, 10.1007/s00726-014-1790-3 · DOI
  31. Walczak, 2012, Kynurenic acid enhances expression of p21 Waf1/Cip1 in colon cancer HT-29 cells, Pharmacol. Rep., 64, 745, 10.1016/S1734-1140(12)70870-8 · DOI

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Metadata

Authors
Sinan, K. I., Chiavaroli, A., Orlando, G., Bene, K., Zengin, G., Cziáky, Z., Jekő, J., Fawzi Mahomoodally, M., Picot-Allain, M. C. N., Menghini, L., Recinella, L., Brunetti, L., Leone, S., Ciferri, M. C., Di Simone, S., & Ferrante, C.
Affiliations
  • Selçuk University (TR)
  • University of Chieti-Pescara (IT)
  • Laboratoire de Botanique et Phytothérapie, Unité de Formation et de Recherche Sciences de la Nature, 02 BP 801 Abidjan 02, Université Nangui Abrogoua, Abidjan 02, Cote D’Ivoire
  • Department of Biology, Science Faculty, Selcuk Universtiy, Campus, Konya, 42130 Konya, Turkey
  • University of Nyíregyháza (HU)
  • Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
  • University of Mauritius (MU)
  • Department of Pharmacy, “G. d’Annunzio” University Chieti-Pescara, 66100 Chieti, Italy
Corresponding author
Orlando, Giustino
ORCID
Venue
Antioxidants
Publisher
MDPI AG
Published
2020-02-17
Volume
9
Issue
2
Pages
163
ISSN
2076-3921 (ISSN-L)
DOI
10.3390/antiox9020163
PMC
PMC7094211
OpenAlex
W3006014100
Cited by
25
References
1
Citations by year
2026: 3 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 2 · 2020: 4
FWCI
2.42
Type
journal article
Language
en
Open access
Licence
Locations
  • Antioxidants (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
  • Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
  • Physical Sciences › Chemistry › Organic Chemistry › Phytochemical Studies and Bioactivities
Keywords
Bersama abyssinica, Melianthaceae, stem bark extract, antioxidant activity, enzyme inhibition, antiproliferative activity, HCT116 cells, kynurenic acid, acetylcholinesterase inhibition, α-glucosidase inhibition, mangiferin, vitexin, isovitexin, phenolic compounds, flavonoids, saponins, phytochemical profiling

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