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Cited by 161Phytochemicals and Antioxidant ActivitiesPhytochemistry and Biological ActivitiesMedicinal Plant Research

Antioxidant Activities and Polyphenolic Contents of Three Selected Micromeria Species from Croatia

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Vladimir-Knežević, Sanda, Blažeković, Biljana, Bival Štefan, Maja, Alegro, Antun, Kőszegi, Tamás, Petrik, József·2011·Molecules
DOI 10.3390/molecules16021454PMC PMC6259752
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Abstract

Antioxidant activities of three selected Micromeria species growing in Croatia (M. croatica, M. juliana and M. thymifolia) were evaluated using five different antioxidant assays, in comparison with plant polyphenolic constituents and reference antioxidants. All studied ethanolic extracts exhibited considerable activity to scavenge DPPH and hydroxyl free radicals, reducing power, iron chelating ability and total antioxidant capacity in the order: M. croatica > M. juliana > M. thymifolia. Total polyphenol (9.69–13.66%), phenolic acid (5.26–6.84%), flavonoid (0.01–0.09%) and tannin (3.07–6.48%) contents in dried plant samples were determined spectrophotometrically. A strong positive correlation between antioxidant activities and contents of phenolic acids and tannins was found, indicating their responsibility for effectiveness of tested plants. Our findings established Micromeria species as a rich source of antioxidant polyphenols, especially the endemic M. croatica.

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  • The FRAP assay is a well-known, reliable approach that measures ferric reducing ability in a redox-linked colorimetric reaction that reduces Fe3+ to Fe2+.

    “The FRAP assay is a well-known, reliable approach that measures ferric reducing ability in a redox-linked colorimetric reaction that reduces Fe3+ to Fe2+ [78–81].”

    From Comparative and Correlational Evaluation of the Phytochemical Constituents and Antioxidant Activity of <i>Musa sinensis</i> L. and <i>Musa paradisiaca</i> L. Fruit Compartments (Musaceae) (2020) · cites this paper as [78]

    Section 2.2 describes a colorimetric reducing-power assay in which antioxidants reduce an Fe3+/ferricyanide complex to the ferrous form, measured by absorbance at 700 nm. Section 3.4.3 identifies the method used as Oyaizu’s reducing-power assay; our copy does not identify it as FRAP or establish that FRAP is well-known and reliable. No page numbers are provided in our copy. (LLM, conf=0.83)

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Authors
Vladimir-Knežević, S., Blažeković, B., Bival Štefan, M., Alegro, A., Kőszegi, T., & Petrik, J.
Affiliations
  • Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, HR-10000 Zagreb, Croatia
  • University of Zagreb (HR)
  • University of Pecs (HU)
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2011-02-10
Volume
16
Issue
2
Pages
1454-1470
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules16021454
PMC
PMC6259752
OpenAlex
W2075280137
Cited by
161
References
1
Citations by year
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FWCI
7.30
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
Keywords
Micromeria, Micromeria juliana, antioxidant activity, polyphenols, phenolic acids, tannins, flavonoids, DPPH radical scavenging, hydroxyl radical scavenging, reducing power, iron chelation, total antioxidant capacity, ethanolic plant extract, endemic plants, Croatian flora
MeSH terms
Antioxidants, Flavonoids, Phenols, Plant Extracts, Tannins, Free Radical Scavengers, Croatia, Lamiaceae, Polyphenols, Hydroxybenzoates

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