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Cited by 51Plant tissue culture and regenerationPlant Molecular Biology ResearchFlowering Plant Growth and Cultivation

Cytokinin-Based Tissue Cultures for Stable Medicinal Plant Production: Regeneration and Phytochemical Profiling of Salvia bulleyana Shoots

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Grzegorczyk-Karolak, Izabela, Hnatuszko-Konka, Katarzyna, Krzemińska, Marta, Olszewska, Monika A., Owczarek, Aleksandra·2021·Biomolecules
DOI 10.3390/biom11101513PMC PMC8533636
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Abstract

Salvia bulleyana is a rare Chinese medicinal plant that due to the presence of polyphenols lowers the risk of some chronic diseases especially those related to the cardiovascular system. The present study examines the organogenic competence of various combinations and concentrations of plant growth regulators to develop an efficient protocol for in vitro regeneration of S. bulleyana via leaf explants, maintaining the high production of active constituents. The purpose of the study was also to assess the possibilities of using a cytokinin-based regeneration to effectively produce therapeutic compounds. The adventitious shoot formation was observed through direct organogenesis on media with purine derivatives (meta-topolin, mT and benzylaminopurine, BAP), and through indirect organogenesis on media with urea derivatives (tidiazuron, TDZ and forchlorfenuron, CPPU). The highest regeneration frequency (95%) with 5.2 shoots per explant was obtained on leaves cultured on Murashige and Skoog (MS) medium containing 0.1 mg/L naphthalene-1-acetic acid (NAA) and 2 mg/L BAP. Following inter simple sequence repeat (ISSR) marker-based profiling, the obtained organogenic shoot lines revealed a similar banding pattern to the mother line, with total variability of 4.2–13.7%, indicating high level of genetic stability. The similar genetic profile of the studied lines translated into similar growth parameters. Moreover, HPLC analysis revealed no qualitative differences in the profile of bioactive metabolites; also, the total polyphenol content was similar for different lines, with the exception of the shoots obtained in the presence of CPPU that produced higher level of bioactive compounds. This is the first report of an effective and rapid in vitro organogenesis protocol for S. bulleyana, which can be efficiently employed for obtaining stable cultures rich in bioactive metabolites.

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  • ISSR markers, applied comparatively between plantlets and mother plant, generally revealed the clonal nature of analyzed regenerants, highlighting genetic fidelity of in vitro propagation, even after several years.

    “However, the ISSR markers, applied comparatively between the plantlets and the mother plant revealed, in general, the clonal nature of the analyzed regenerants, highlighting the genetic fidelity of in vitro propagation, even after several years [70,71,72].”

    From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [72]

    Section 3.3 (page unavailable) reports that ISSR comparisons of control shoots and regenerants showed mainly monomorphic patterns and high genetic similarity, supporting the general claim of clonal fidelity. The paper does not establish fidelity after several years: Section 2.3 describes assessment after 9–11 passages, and Section 2.5 describes 10 passages as approximately one year. (LLM, conf=0.88)

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References 41 · Cited by 51

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Authors
Grzegorczyk-Karolak, I., Hnatuszko-Konka, K., Krzemińska, M., Olszewska, M. A., & Owczarek, A.
Affiliations
  • Medical University of Lodz (PL)
  • University of Łódź (PL)
Corresponding author
Grzegorczyk-Karolak, Izabela
ORCID
Venue
Biomolecules
Publisher
MDPI AG
Published
2021-10-14
Volume
11
Issue
10
Pages
1513
ISSN
2218-273X (ISSN-L)
DOI
10.3390/biom11101513
PMC
PMC8533636
OpenAlex
W3207772599
Cited by
51
References
41
Citations by year
2026: 11 · 2025: 13 · 2024: 9 · 2023: 11 · 2022: 7
FWCI
2.12
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomolecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
Keywords
in vitro organogenesis, shoot regeneration, leaf explants, benzylaminopurine, CPPU, naphthaleneacetic acid, ISSR markers, genetic stability, polyphenols, bioactive metabolites, medicinal plant cultivation, micropropagation
MeSH terms
Benzyl Compounds, Culture Media, Cytokinins, Humans, Medicine, Chinese Traditional, Plant Growth Regulators, Plants, Medicinal, Purines, Regeneration, Plant Leaves, Plant Shoots, Salvia, Tissue Culture Techniques

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