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Cited by 15Sperm and Testicular FunctionReproductive Health and TechnologiesReproductive Biology and Fertility

Analyzing the Differential Impact of Semen Preparation Methods on the Outcomes of Assisted Reproductive Techniques

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Bibi, Riffat, Jahan, Sarwat, Afsar, Tayyaba, Almajwal, Ali, Hammadeh, Mohamad Eid, Amor, Houda, Abusharha, Ali, Razak, Suhail·2023·Biomedicines
DOI 10.3390/biomedicines11020467PMC PMC9953211
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Abstract

Sperm separation plays a critical role in assisted reproductive technology. Based on migration, density gradient centrifugation and filtration, a properly selected sperm could help in increasing assisted reproductive outcomes in teratozoospermia (TZs). The current study aimed to assess the prognostic value of four sperm selection techniques: density gradient centrifugation (DGC), swim-up (SU), DGC-SU and DGC followed by magnetic-activated cell sorting (DGC-MACS). These were evaluated using spermatozoa functional parameters. A total of 385 infertile couples underwent the procedure of intracytoplasmic sperm injection (ICSI), with an isolated teratozoospermia in the male partner. Semen samples were prepared by using one of the mentioned sperm preparation techniques. The improvements in the percentage of normal mature spermatozoa, rate of fertilization, cleavage, pregnancy and the number of live births were assessed. The normal morphology, spermatozoa DNA fragmentation (SDF) and chromatin maturity checked by using chromomycin A3 (CMA3) with DGC-MACS preparation were better compared to the other three methods. Embryo cleavage, clinical pregnancy and implantation were better improved in the DGC-MACS than in the other tested techniques. The DGC-MACS technique helped in the selection of an increased percentage of normal viable and mature sperm with intact chromatin integrity in patients with teratozoospermia.

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  • Sperm selection with density gradient centrifugation followed by magnetic-activated cell sorting recovered a higher percentage of mature, viable sperm with intact chromatin among patients with isolated teratozoospermia.

    “Along similar lines, sperm selection with density gradient centrifugation followed by magnetic-activated cell sorting recovered a higher percentage of mature, viable sperm with intact chromatin among patients with isolated teratozoospermia (93).”

    From Isolated teratozoospermia: revisiting its relevance in male infertility: a narrative review (2024) · cites this paper as [93]

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Authors
Bibi, R., Jahan, S., Afsar, T., Almajwal, A., Hammadeh, M. E., Amor, H., Abusharha, A., & Razak, S.
Affiliations
  • Quaid-i-Azam University (PK)
  • King Saud University (SA)
  • Universitätsklinikum des Saarlandes (DE)
Corresponding author
  • Bibi, Riffat
  • Razak, Suhail
ORCID
Venue
Biomedicines
Publisher
MDPI AG
Published
2023-02-06
Volume
11
Issue
2
Pages
467
ISSN
2227-9059 (ISSN-L)
DOI
10.3390/biomedicines11020467
PMC
PMC9953211
OpenAlex
W4319298046
Cited by
15
References
2
Citations by year
2026: 4 · 2025: 6 · 2024: 5
FWCI
3.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomedicines (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Publications of the UdS (Saarland University) (cc-by)
Funders
King Saud University (RSP2023R502)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Health and Technologies
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
Keywords
sperm selection, semen preparation, teratozoospermia, density gradient centrifugation, swim-up, magnetic cell separation, intracytoplasmic sperm injection, sperm DNA fragmentation, chromatin integrity, chromomycin A3, male infertility, fertilization rate, embryo cleavage, clinical pregnancy, live birth rate

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