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In Vitro Anti-Inflammatory Properties of Selected Green Leafy Vegetables
Open accessAbstract
The study investigated the anti-inflammatory activity of the hydro methanolic extract of six leafy vegetables, namely Cassia auriculata, Passiflora edulis, Sesbania grandiflora, Olax zeylanica, Gymnema lactiferum, and Centella asiatica. The anti-inflammatory activity of methanolic extracts of leafy vegetables was evaluated using four in vitro-based assays: hemolysis inhibition, proteinase inhibition, protein denaturation inhibition, and lipoxygenase inhibition. Results showed that the percent inhibition of hemolysis from these leaf extracts (25–100 µg/mL dry weight basis (DW)) was within the range from 5.4% to 14.9%, and the leaves of P. edulis and O. zeylanica showed a significantly higher (p < 0.05) inhibition levels. Percent inhibition of protein denaturation of these leafy types was within the range of 36.0–61.0%, and the leaf extract of C. auriculata has exhibited a significantly higher (p < 0.05) inhibition level. Proteinase inhibitory activity of these leaf extracts was within the range of 20.2–25.9%. The lipoxygenase inhibition was within the range of 3.7–36.0%, and the leaf extract of G. lactiferum showed an improved ability to inhibit lipoxygenase activity. In conclusion, results revealed that all the studied leaves possess anti-inflammatory properties at different levels, and this could be due to the differences in the composition and concentration of bioactive compounds.
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“In addition, C. asiatica has been shown to reduce inflammation by inhibiting lipoxygenase activity and lessening proteinase activity, thus inhibiting protein denaturation [40].”
From A systematic review of the effect of Centella asiatica on wound healing (2022) · cites this paper as [40]
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Cites
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Cited by
Sorted by citations
A systematic review of the effect of Centella asiatica on wound healing
Arribas-López, E., Zand, N., Ojo, O., Snowden, M. J., & Kochhar, T. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph19063266 · Cited by 143
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El-Deeb, B., El-Raheem, W. M. A., & Elbeltagi, S. (2023) · Scientific Reports · doi:10.1038/s41598-023-46287-6 · Cited by 112
Hamid, S. J., & Salih, T. M. (2022) · Drug Design Development and Therapy · doi:10.2147/dddt.s364746 · Cited by 92
Prasathkumar, M., Raja, K., Vasanth, K., Khusro, A., Sadhasivam, S., Sahibzada, M. U. K., AbdelGawwad, M. R., Farraj, D. A. A., & Elshikh, M. S. (2021) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2021.103345 · Cited by 86
Metadata
- Authors
- Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., & Rupasinghe, H. P. V.
- Affiliations
- University of Sri Jayewardenepura (LK)
- Dalhousie University (CA)
- Corresponding author
- Gunathilake, K. D. P. P.
- Venue
- Biomedicines
- Publisher
- MDPI AG
- Published
- 2018-11-19
- Volume
- 6
- Issue
- 4
- Pages
- 107
- ISSN
- 2227-9059 (ISSN-L)
- PMC
- PMC6316011
- OpenAlex
- W2901135781
- Cited by
- 330
- References
- 30
- Citations by year
- 2026: 45 · 2025: 67 · 2024: 50 · 2023: 59 · 2022: 61 · 2021: 28 · 2020: 14 · 2019: 5
- FWCI
- 10.40
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Biomedicines (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, other-oa)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Wayamba University of Sri Lanka
- National Science Foundation of Sri Lanka
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Keywords
- anti-inflammatory activity, leafy vegetables, methanolic leaf extract, hemolysis inhibition, protease inhibition, protein denaturation inhibition, lipoxygenase inhibition, Passiflora edulis, Centella asiatica, Cassia auriculata, Sesbania grandiflora, bioactive compounds
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- Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., & Rupasinghe, H. P. V. (2018). In Vitro Anti-Inflammatory Properties of Selected Green Leafy Vegetables. Biomedicines. https://doi.org/10.3390/biomedicines6040107
