A Comprehensive Review of Calcium Electroporation—A Novel Cancer Treatment Modality
Open accessAbstract
Calcium electroporation is a potential novel anti-cancer treatment where high calcium concentrations are introduced into cells by electroporation, a method where short, high voltage pulses induce transient permeabilisation of the plasma membrane allowing passage of molecules into the cytosol. Calcium is a tightly regulated, ubiquitous second messenger involved in many cellular processes including cell death. Electroporation increases calcium uptake leading to acute and severe ATP depletion associated with cancer cell death. This comprehensive review describes published data about calcium electroporation applied in vitro, in vivo, and clinically from the first publication in 2012. Calcium electroporation has been shown to be a safe and efficient anti-cancer treatment in clinical studies with cutaneous metastases and recurrent head and neck cancer. Normal cells have been shown to be less affected by calcium electroporation than cancer cells and this difference might be partly induced by differences in membrane repair, expression of calcium transporters, and cellular structural changes. Interestingly, both clinical data and preclinical studies have indicated a systemic immune response induced by calcium electroporation. New cancer treatments are needed, and calcium electroporation represents an inexpensive and efficient treatment with few side effects, that could potentially be used worldwide and for different tumor types.
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Calcium electroporation is thought to work by the same mechanism as electrochemotherapy and uses calcium because of its role as a second messenger for various processes resulting in cell death.
“For instance, calcium electroporation is thought to work by the same mechanism as electrochemotherapy, and it uses calcium because of its role as a second messenger for various processes resulting in cell death [78].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [78]
Cancer cells may be more sensitive to electroporation because they are less equipped to repair and restore the plasma membrane after damage.
“A study by Frandsen and colleagues investigated the mechanisms of calcium electroporation, leading the authors to hypothesize that cancer cells may be more sensitive to electroporation because they are less equipped to repair and restore the plasma membrane after damage [78,80].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [78]
Electrochemotherapy has proven effective in targeting cancer cells that are otherwise resistant to the chemotherapy being administered.
“In particular, electrochemotherapy has proven effective in targeting cancer cells that are otherwise resistant to the chemotherapy being administered [78].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [78]
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Flagged first, then by citations
Research works printed but not linked: 1
- [113]Gibot L.A.M. Rols M.P. Calcium delivery by electroporation induces in vitro cell death through mitochondrial dysfunction without genetoxicity Proceedings of the 3rd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies Toulouse, France 3–6 September 2019
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Sorted by citations
Permeabilizing Cell Membranes with Electric Fields
Aguilar, A. A., Ho, M. C., Chang, E., Carlson, K. W., Natarajan, A., Marciano, T., Bomzon, Z., & Patel, C. B. (2021) · Cancers · doi:10.3390/cancers13092283 · Cited by 76 · see citing works
- Authors
- Frandsen, S. K., Vissing, M., & Gehl, J.
- Affiliations
- Zealand University Hospital (DK)
- University of Copenhagen (DK)
- Corresponding author
- Gehl, Julie
- Venue
- Cancers
- Publisher
- MDPI AG
- Published
- 2020-01-25
- Volume
- 12
- Issue
- 2
- Pages
- 290
- ISSN
- 2072-6694 (ISSN-L)
- PMC
- PMC7073222
- OpenAlex
- W3003142605
- Cited by
- 132
- References
- 131
- Citations by year
- 2026: 9 · 2025: 19 · 2024: 28 · 2023: 23 · 2022: 19 · 2021: 26 · 2020: 7 · 2019: 1
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Cancers (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Zealand University Hospital (EPeOnc, the Enhanced Perioperative Oncology Consortium)
- Region Sjællands Sundhedsvidenskabelige Forskningsfond (R17A36B30)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Plasma Applications and Diagnostics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Keywords
- calcium electroporation, electroporation, cancer cell death, calcium uptake, membrane permeabilization, ATP depletion, cutaneous metastasis, recurrent head and neck cancer, immune response, calcium transporters, membrane repair, second messengers
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