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Cited by 9Muscle Physiology and DisordersChronic Obstructive Pulmonary Disease (COPD) ResearchMuscle metabolism and nutrition

Response to Electrostimulation Is Impaired in Muscle Cells from Patients with Chronic Obstructive Pulmonary Disease

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Catteau, Matthias, Passerieux, Emilie, Blervaque, Léo, Gouzi, Farés, Ayoub, Bronia, Hayot, Maurice, Pomiès, Pascal·2021·Cells
DOI 10.3390/cells10113002PMC PMC8616440
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Abstract

Among the comorbidities associated with chronic obstructive pulmonary disease (COPD), skeletal muscle weakness and atrophy are known to affect patient survival rate. In addition to muscle deconditioning, various systemic and intrinsic factors have been implicated in COPD muscle dysfunction but an impaired COPD muscle adaptation to contraction has never been extensively studied. We submitted cultured myotubes from nine healthy subjects and nine patients with COPD to an endurance-type protocol of electrical pulse stimulation (EPS). EPS induced a decrease in the diameter, covered surface and expression of MHC1 in COPD myotubes. Although the expression of protein degradation markers was not affected, expression of the protein synthesis marker mTOR was not induced in COPD compared to healthy myotubes after EPS. The expression of the differentiation markers p16INK4a and p21 was impaired, while expression of Myf5 and MyoD tended to be affected in COPD muscle cells in response to EPS. The expression of mitochondrial biogenesis markers PGC1α and MFN2 was affected and expression of TFAM and COX1 tended to be reduced in COPD compared to healthy myotubes upon EPS. Lipid peroxidation was increased and the expression of the antioxidant enzymes SOD2 and GPx4 was affected in COPD compared to healthy myotubes in response to EPS. Thus, we provide evidence of an impaired response of COPD muscle cells to contraction, which might be involved in the muscle weakness observed in patients with COPD.

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  • In vitro, mitochondrial biogenesis markers PGC1α (and to a lesser extent TFAM and COX1) and MFN2 have been shown to be reduced in cultured myotubes from COPD patients compared to healthy subjects upon electrical stimulation.

    “mitochondrial biogenesis markers PGC1α (and to a lesser extent TFAM and COX1) and MFN2 have been shown to be reduced in cultured myotubes from COPD patients compared to healthy subjects upon electrical stimulation [92].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [92]

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Authors
Catteau, M., Passerieux, E., Blervaque, L., Gouzi, F., Ayoub, B., Hayot, M., & Pomiès, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • PhyMedExp, University of Montpellier—INSERM—CNRS—CHRU Montpellier, 34295 Montpellier, France
Corresponding author
Pomiès, Pascal
ORCID
Venue
Cells
Publisher
MDPI AG
Published
2021-11-03
Volume
10
Issue
11
Pages
3002
ISSN
2073-4409 (ISSN-L)
DOI
10.3390/cells10113002
PMC
PMC8616440
OpenAlex
W3209880202
Cited by
9
References
2
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 3
FWCI
0.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Cells (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
French Ministry of Public Health and Montpellier University Hospital (PHRCI)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
chronic obstructive pulmonary disease, skeletal muscle weakness, muscle atrophy, electrical pulse stimulation, endurance training, myotubes, muscle fiber type shift, mTOR signaling, myogenesis, mitochondrial biogenesis, lipid peroxidation, oxidative stress, manganese superoxide dismutase, GPX4, PGC-1α, MyoD, Myf5
MeSH terms
Antioxidants, Organelle Biogenesis, Cell Differentiation, Electric Stimulation Therapy, Female, Humans, Male, Middle Aged, Lipid Peroxidation, Biomarkers, Case-Control Studies, Oxidative Stress, Muscle Fibers, Skeletal, Pulmonary Disease, Chronic Obstructive, Muscle Cells, Proteolysis

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