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Cited by 7Muscle Physiology and DisordersCardiovascular and exercise physiologyCongenital heart defects research

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Fusion of Normoxic- and Hypoxic-Preconditioned Myoblasts Leads to Increased Hypertrophy

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Pircher, Tamara, Wackerhage, Henning, Akova, Elif, Böcker, Wolfgang, Aszodi, Attila, Saller, Maximilian M.·2022·Cells
DOI 10.3390/cells11061059PMC PMC8947054
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Abstract

Injuries, high altitude, and endurance exercise lead to hypoxic conditions in skeletal muscle and sometimes to hypoxia-induced local tissue damage. Thus, regenerative myoblasts/satellite cells are exposed to different levels and durations of partial oxygen pressure depending on the spatial distance from the blood vessels. To date, it is unclear how hypoxia affects myoblasts proliferation, differentiation, and particularly fusion with normoxic myoblasts. To study this, we investigated how 21% and 2% oxygen affects C2C12 myoblast morphology, proliferation, and myogenic differentiation and evaluated the fusion of normoxic- or hypoxic-preconditioned C2C12 cells in 21% or 2% oxygen in vitro. Out data show that the long-term hypoxic culture condition does not affect the proliferation of C2C12 cells but leads to rounder cells and reduced myotube formation when compared with myoblasts exposed to normoxia. However, when normoxic- and hypoxic-preconditioned myoblasts were differentiated together, the resultant myotubes were significantly larger than the control myotubes. Whole transcriptome sequencing analysis revealed several novel candidate genes that are differentially regulated during the differentiation under normoxia and hypoxia in mixed culture conditions and may thus be involved in the increase in myotube size. Taken together, oxygen-dependent adaption and interaction of myoblasts may represent a novel approach for the development of innovative therapeutic targets.

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Metadata

Authors
Pircher, T., Wackerhage, H., Akova, E., Böcker, W., Aszodi, A., & Saller, M. M.
Affiliations
  • Institut für Muskuloskelettale Medizin (DE)
  • Technical University of Munich (DE)
  • Department of Orthopaedics and Trauma Surgery, Musculoskeletal University Center Munich (MUM), Ludwig-Maximilians-University (LMU), Fraunhoferstraße 20, 82152 Planegg-Martinsried, Germany
Corresponding author
Saller, Maximilian M.
ORCID
Venue
Cells
Publisher
MDPI AG
Published
2022-03-21
Volume
11
Issue
6
Pages
1059
ISSN
2073-4409 (ISSN-L)
DOI
10.3390/cells11061059
PMC
PMC8947054
OpenAlex
W4220852145
Cited by
7
References
1
Citations by year
2025: 1 · 2024: 2 · 2023: 2 · 2022: 2
FWCI
0.57
Type
journal article
Language
en
Open access
Licence
Locations
  • Cells (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Electronic Theses of LMU Munich (Ludwig-Maximilians-Universität München) (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion)
  • Open access LMU (Ludwid Maxmilian's Universitat Munchen) (submittedVersion, cc-by)
  • mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology (medical) › Cardiovascular and exercise physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
Keywords
myoblast fusion, hypoxia, skeletal muscle hypertrophy, C2C12 myoblasts, myogenesis, myotube formation, oxygen partial pressure, satellite cells, transcriptome, muscle regeneration
MeSH terms
Hypoxia, Humans, Hypertrophy, Oxygen, Muscle Fibers, Skeletal, Myoblasts

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