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Substantial Dysregulation of miRNA Passenger Strands Underlies the Vascular Response to Injury

Pinel, Karine, Diver, Louise A., White, Katie, McDonald, Robert A., Baker, Andrew H.·2019·Cells
DOI 10.3390/cells8020083PMC PMC6406808
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Abstract

Vascular smooth muscle cell (VSMC) dedifferentiation is a common feature of vascular disorders leading to pro-migratory and proliferative phenotypes, a process induced through growth factor and cytokine signaling cascades. Recently, many studies have demonstrated that small non-coding RNAs (miRNAs) can induce phenotypic effects on VSMCs in response to vessel injury. However, most studies have focused on the contribution of individual miRNAs. Our study aimed to conduct a detailed and unbiased analysis of both guide and passenger miRNA expression in vascular cells in vitro and disease models in vivo. We analyzed 100 miRNA stem loops by TaqMan Low Density Array (TLDA) from primary VSMCs in vitro. Intriguingly, we found that a larger proportion of the passenger strands was significantly dysregulated compared to the guide strands after exposure to pathological stimuli, such as platelet-derived growth factor (PDGF) and IL-1α. Similar findings were observed in response to injury in porcine vein grafts and stent models in vivo. In these studies, we reveal that the miRNA passenger strands are predominantly dysregulated in response to vascular injury.

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  • The phenomena of miRNA arm switching, so far only observed in cancer, extends to healthy mammalian tissues.

    “This highlights the complexity of tissue-dependent miRNA biogenesis and indicates that the phenomena of miRNA arm switching, so far only observed in cancer, extends to healthy mammalian tissues (43–45).”

    From A mouse tissue atlas of small noncoding RNA (2020) · cites this paper as [44]

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Cited by

Sorted by citations

Authors
Pinel, K., Diver, L. A., White, K., McDonald, R. A., & Baker, A. H.
Affiliations
Institute of Cardiovascular and Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, Glasgow G12 8TA, UK
ORCID
Baker, Andrew H.
Venue
Cells
Publisher
MDPI AG
Published
2019-01-23
Volume
8
Issue
2
Pages
83
ISSN
2073-4409
DOI
10.3390/cells8020083
PMC
PMC6406808
Cited by
13
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (vor)

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