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Cited by 182Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsLysosomal Storage Disorders Research

Senescence-Associated β-Galactosidase Detection in Pathology

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Valieva, Yana, Ivanova, Elena, Fayzullin, Alexey, Kurkov, Alexander, Igrunkova, Alexandra·2022·Diagnostics
DOI 10.3390/diagnostics12102309PMC PMC9599972
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Abstract

Activity of β-galactosidase at pH 6 is a classic maker of senescence in cellular biology. Cellular senescence, a state of highly stable cell cycle arrest, is often compared to apoptosis as an intrinsic tumor suppression mechanism. It is also thought that SA-β-gal is crucial in malignant cell transformation. High levels of senescence-associated β-galactosidase (SA-β-gal) can be found in cancer and benign lesions of various localizations making the enzyme a highly promising diagnostic marker for visualization of tumor margins and metastases. These findings facilitate the research of therapy induced senescence as a promising therapeutic strategy. In this review, we address the need to collect and analyze the bulk of clinical and biological data on SA-β-gal mechanisms of action to support wider implementation of this enzyme in medical diagnostics. The review will be of interest to pathologists, biologists, and biotechnologists investigating cellular senescence for purposes of regenerative medicine and oncology.

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  • Certain senescence markers based on post-translational modifications, enzymatic activity, or protein translocations are more sensitive to tissue collection times and fixation methods.

    “It is likely that certain senescence markers, such as those based on post-translational modifications, enzymatic activity, or protein translocations, are more sensitive to tissue collection times and fixation methods159,160.”

    From Guidelines for minimal information on cellular senescence experimentation in vivo (2024) · cites this paper as [159]

    The Introduction describes detecting senescence-associated β-galactosidase through its enzymatic activity, and Future Prospects discusses staining non-processed tissue. Neither passage says that this or other senescence markers are more sensitive to tissue collection times or fixation methods. Searches for fixation, sample handling, post-translational modifications, and protein translocations found no passage addressing that comparison; page numbers were not available in our copy. (LLM, conf=0.73)

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Cited by

Sorted by citations

Authors
Valieva, Y., Ivanova, E., Fayzullin, A., Kurkov, A., & Igrunkova, A.
Affiliations
  • Sechenov University (RU)
  • Department of Experimental Morphology and Biobanking, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia
Corresponding author
Fayzullin, Alexey
ORCID
Venue
Diagnostics
Publisher
MDPI AG
Published
2022-09-25
Volume
12
Issue
10
Pages
2309
ISSN
2075-4418 (ISSN-L)
DOI
10.3390/diagnostics12102309
PMC
PMC9599972
OpenAlex
W4297147613
Cited by
182
References
8
Citations by year
2026: 47 · 2025: 68 · 2024: 47 · 2023: 20
FWCI
4.64
Type
journal article
Language
en
Open access
Licence
Locations
  • Diagnostics (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Russian Science Foundation (21-75-00081, 075-15-2022-304)
  • Ministry of Science and Higher education of Russian Federation (21-75-00081, 075-15-2022-304)
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Health Sciences › Medicine › Physiology › Lysosomal Storage Disorders Research
Keywords
senescence-associated β-galactosidase, cellular senescence, tumor suppression, therapy-induced senescence, tumor margin visualization, metastasis detection, cancer diagnosis, benign lesions, regenerative medicine

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