No Waste from Waste: Membrane-Based Fractionation of Second Cheese Whey for Potential Nutraceutical and Cosmeceutical Applications, and as Renewable Substrate for Fermentation Processes Development
Open accessAbstract
Second cheese whey (SCW) derived from buffalo milk is the main by-product of the mozzarella cheese dairy industry. The objective of this study was to develop a membrane-based purification procedure to obtain specific fractions from SCW and assess their biological and applicative potential. Special interest was paid to the proteins and newly identified health-promoting compounds that could be recovered and used as value-added products in different sectors of food and pharmaceutical industries. SCW has been treated, sequentially, with microfiltration (MF), ultrafiltration (UF), and nanofiltration (NF) membranes giving the possibility to obtain three different fractions, namely retentates recovered on 100 and 10 kDa (R100 and R10) and a nanofiltration retentate (RNF). These retentates were compared for their ability to preserve human keratinocytes from dehydration, to form protein-based films by casting, and finally they were used for probiotic cultivations as the main substrate. Results showed that Lactobacillus rhamnosus could grow without any further additional nutrient up to 2.2 ± 0.3 × 109 CFU/mL in the RNF medium. Dehydration tests on HaCat cells proved R100 as the most efficient fraction in preserving cell viability from this specific stress. R10, after diafiltrations, formed transparent films with improved features when glycerol was added as a plasticizer.
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Approximately 300-320 million tons of whey ends up in wastewaters every year.
“Every year, approximately 300–320 million tons of whey ends up in wastewaters [141].”
From Use of Waste from the Food Industry and Applications of the Fermentation Process to Create Sustainable Cosmetic Products: A Review (2024) · cites this paper as [141]
The Introduction estimates 300–320 million tons per year as total worldwide whey production in 2020, not the amount entering wastewaters. It says approximately 50% of worldwide whey production is normally disposed of without being utilized, contrary to the claim that the entire 300–320 million tons ends up in wastewaters. (LLM, conf=0.88)
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Experimental — accuracy not yet measuredBioactive compounds isolated from animal-based waste are mainly peptides.
“Bioactive compounds isolated from animal-based waste include mainly peptides.”
From Use of Waste from the Food Industry and Applications of the Fermentation Process to Create Sustainable Cosmetic Products: A Review (2024) · cites this paper as [141]
The Discussion (page not provided) describes recovering bioactive molecules from buffalo second cheese whey and emphasizes protein-rich fractions. The Introduction (page not provided) mentions macropeptides among whey components, but the paper does not establish that bioactive compounds isolated from animal-based waste generally are mainly peptides. (LLM, conf=0.85)
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- Authors
- Alfano, A., D’ambrosio, S., Cimini, D., Falco, L., D’Agostino, M., Finamore, R., & Schiraldi, C.
- Affiliations
- University of Campania "Luigi Vanvitelli" (IT)
- Corresponding author
- Schiraldi, Chiara
- Venue
- Fermentation
- Publisher
- MDPI AG
- Published
- 2022-10-04
- Volume
- 8
- Issue
- 10
- Pages
- 514
- ISSN
- 2311-5637 (ISSN-L)
- OpenAlex
- W4303699789
- Cited by
- 15
- References
- 31
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 5 · 2023: 1
- FWCI
- 0.73
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Fermentation (publishedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Archivio Istituzionale della Ricerca - Università degli Studi della Campania "Luigi Vanvitelli" (submittedVersion)
- Funders
- MIUR (PON03PE00060_2, ARS01_01166)
- PON BIONUTRA (PON03PE00060_2, ARS01_01166)
- Project DM 1062 del 10/08/2021 Azione IV.6—Contratti di ricerca su tematiche green (PON03PE00060_2, ARS01_01166)
- Topics
- Physical Sciences › Environmental Science › Water Science and Technology › Membrane Separation Technologies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- buffalo milk, membrane fractionation, microfiltration, ultrafiltration, nanofiltration, whey protein, protein films, Lactobacillus rhamnosus, HaCaT keratinocytes, nutraceuticals, cosmeceuticals, dairy by-products, value-added products
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