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Cited by 177Plant tissue culture and regenerationFlowering Plant Growth and CultivationGrowth and nutrition in plants

An Academic and Technical Overview on Plant Micropropagation Challenges

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Abdalla, Neama, El-Ramady, Hassan, Seliem, Mayada K., El-Mahrouk, Mohammed E., Taha, Naglaa, Bayoumi, Yousry, Shalaby, Tarek A., Dobránszki, Judit·2022·Horticulturae
DOI 10.3390/horticulturae8080677
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Abstract

The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

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  • Micropropagation has expanded to include various crops, fruit plants, vegetables, trees, medicinal and aromatic plants, and is now a multibillion-dollar industry worldwide.

    “Micropropagation, initially applied to ornamental plants [5], has expanded to include various crops, fruit plants, vegetables, trees, and medicinal and aromatic plants, and is now a multibillion-dollar industry worldwide [6].”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [5]

    The paper discusses micropropagation across diverse plants, including fruit crops, trees, and medicinal plants (References; Section 4.4), and describes its commercial use (General Discussion). It does not give a worldwide market value or say the industry is worth multiple billions of dollars. The separate claim about its initial use in ornamentals is attached to [5], not this source. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Plant tissue culture is an alternative method of conventional vegetative propagation.

    “Thus, plant tissue culture is an alternative method of conventional vegetative propagation [4,5].”

    From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [5]

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Authors
Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., Shalaby, T. A., & Dobránszki, J.
Affiliations
  • University of Debrecen (HU)
  • Kafrelsheikh University (EG)
  • Ornamental and Floriculture Department, Horticulture Research Institute, Antoniadis, Alexandria 21599, Egypt
  • Agricultural Research Center (EG)
Corresponding author
  • Abdalla, Neama
  • El-Ramady, Hassan
ORCID
Venue
Horticulturae
Publisher
MDPI AG
Published
2022-07-25
Volume
8
Issue
8
Pages
677
ISSN
2311-7524 (ISSN-L)
DOI
10.3390/horticulturae8080677
OpenAlex
W4287835779
Cited by
177
References
148
Citations by year
2026: 40 · 2025: 72 · 2024: 34 · 2023: 27 · 2022: 4
FWCI
11.18
Type
journal article
Language
en
Open access
Licence
Locations
  • Horticulturae (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Research, Development and Innovation Fund of Hungary (TKP2021-EGA-20, TKP2021-EGA, AK-00184-003/2021)
  • Hungarian Tempus Public Foundation (TPF), Bilateral State Scholarships (TKP2021-EGA-20, TKP2021-EGA, AK-00184-003/2021)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Growth and nutrition in plants
Keywords
micropropagation, tissue culture, contamination, browning, in vitro rooting, somaclonal variation, hyperhydricity, shoot tip necrosis, recalcitrance

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