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Cited by 6Pain Mechanisms and TreatmentsNeuropeptides and Animal PhysiologyPain Management and Opioid Use

Disulfiram Abrogates Morphine Tolerance—A Possible Role of µ-Opioid Receptor-Related G-Protein Activation in the Striatum

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de Corde-Skurska, Anna, Krzascik, Pawel, Lesniak, Anna, Sacharczuk, Mariusz, Nagraba, Lukasz, Bujalska-Zadrozny, Magdalena·2021·International Journal of Molecular Sciences
DOI 10.3390/ijms22084057PMC PMC8071001
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Abstract

One of the key strategies for effective pain management involves delaying analgesic tolerance. Early clinical reports indicate an extraordinary effectiveness of off-label disulfiram—an agent designed for alcohol use disorder—in potentiating opioid analgesia and abrogation of tolerance. Our study aimed to determine whether sustained µ-opioid signaling upon disulfiram exposure contributes to these phenomena. Wistar rats were exposed to acute and chronic disulfiram and morphine cotreatment. Nociceptive thresholds were assessed with the mechanical Randal-Selitto and thermal tail-flick tests. µ-opioid receptor activation in brain structures important for pain processing was carried out with the [35S]GTPγS assay. The results suggest that disulfiram (12.5–50 mg/kg i.g.) augmented morphine antinociception and diminished morphine (25 mg/kg, i.g.) tolerance in a supraspinal, opioid-dependent manner. Disulfiram (25 mg/kg, i.g.) induced a transient enhancement of µ-opioid receptor activation in the periaqueductal gray matter (PAG), rostral ventromedial medulla (RVM), hypothalamus, prefrontal cortex and the dorsal striatum at day 1 of morphine treatment. Disulfiram rescued µ-opioid receptor signaling in the nucleus accumbens and caudate-putamen 14 days following morphine and disulfiram cotreatment. The results of this study suggest that striatal µ-opioid receptors may contribute to the abolition of morphine tolerance following concomitant treatment with disulfiram.

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Authors
de Corde-Skurska, A., Krzascik, P., Lesniak, A., Sacharczuk, M., Nagraba, L., & Bujalska-Zadrozny, M.
Affiliations
  • Department of Pharmacodynamics, Centre for Preclinical Research and Technology, Medical University of Warsaw, Banacha 1b Str., 02-097 Warsaw, Poland
  • Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology, Medical University of Warsaw, Banacha 1b Str., 02-097 Warsaw, Poland
  • Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences (PL)
  • Department of Orthopaedics and Rehabilitation, Medical University of Warsaw, Bursztynowa 2 Str., 04-749 Warsaw, Poland
ORCID
Sacharczuk, Mariusz
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2021-04-14
Volume
22
Issue
8
Pages
4057
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms22084057
PMC
PMC8071001
OpenAlex
W3156230711
Cited by
6
References
76
Citations by year
2026: 2 · 2025: 1 · 2023: 2 · 2022: 1
FWCI
0.47
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ministry of Science and Higher Education in Poland (FW/N/13)
Topics
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
  • Health Sciences › Medicine › Anesthesiology and Pain Medicine › Pain Management and Opioid Use
Keywords
disulfiram, morphine tolerance, mu-opioid receptor, opioid analgesia, G protein signaling, dorsal striatum, periaqueductal gray, rostral ventromedial medulla, nucleus accumbens, caudate-putamen, nociception, analgesic tolerance
MeSH terms
Alcoholism, Analgesics, Opioid, Animals, Corpus Striatum, Disulfiram, Drug Tolerance, Humans, Male, Morphine, Receptors, Opioid, mu, GTP-Binding Proteins, Rats, Pain Management, Gray Matter

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