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Cited by 10Plant Pathogens and Fungal DiseasesFungal and yeast genetics researchFungal Plant Pathogen Control

Comparative transcriptome analysis reveals the effect of the DHN melanin biosynthesis pathway on the appressorium turgor pressure of the poplar anthracnose-causing fungus Colletotrichum gloeosporioides

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Qin, Xinyu, Tian, Chengming, Meng, Fanli·2023·International Journal of Molecular Sciences
DOI 10.3390/ijms24087411PMC PMC10138971
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Abstract

Anthracnose of poplar caused by Colletotrichum gloeosporioides is a leaf disease that seriously affects poplar growth. The pathogen invades the host in the form of adherent cells, which generate turgor pressure through the metabolism of intracellular substances prior to penetrating the epidermis of poplar leaves. In this study, the expansion-related pressure of the mature appressorium of the wild-type C. gloeosporioides was approximately 13.02 ± 1.54 MPa at 12 h, whereas it was 7.34 ± 1.23 MPa and 9.34 ± 2.22 MPa in the melanin synthesis-related gene knockout mutants ΔCgCmr1 and ΔCgPks1, respectively. The CgCmr1 and CgPks1 genes were highly expressed at 12 h in the wild-type control, implying that the DHN melanin biosynthesis pathway may play an important role in the mature appressorium stage. The transcriptome sequencing analysis indicated that the upregulated melanin biosynthesis genes in C. gloeosporioides, such as CgScd1, CgAyg1, CgThr1, CgThr2, and CgLac1, are involved in specific KEGG pathways (i.e., fatty acid biosynthesis, fatty acid metabolism, and biotin metabolism). Therefore, we speculate that the melanin synthesis-related genes and fatty acid metabolism pathway genes contribute to the regulation of the turgor pressure in the mature C. gloeosporioides appressorium, ultimately leading to the formation of infection pegs that enter plant tissues. These observations may reflect the co-evolution of C. gloeosporioides and its host.

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  • In Colletotrichum gloeosporioides, deletion of CgCmr1 or CgPks1 results in melanin-deficient strains with reduced melanin accumulation in appressoria and lower appressorial turgor pressure than wild-type.

    “In C. gloeosporioides, deletion of CgCmr1 or CgPks1 results in melanin-deficient strains, which exhibit reduced melanin accumulation in appressoria and lower appressorial turgor pressure than wild-type [55,56].”

    From Fungal Melanin in Plant Pathogens: Complex Biosynthesis Pathways and Diverse Biological Functions (2025) · cites this paper as [56]

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  1. [1]Li X. Sun H. Pei J. Dong Y. Wang F. Chen H. Sun Y. Wang N. Li H. Li Y. De novo sequencing and comparative analysis of the blueberry transcriptome to discover putative genes related to antioxidants Gene 2012 511 54 61 10.1016/j.gene.2012.09.021 22995346 · DOI
  2. [2]Wang M. Zhou Z. Wu J. Ji Z. Zhang J. Comparative transcriptome analysis reveals significant differences in gene expression between appressoria and hyphae in Colletotrichum gloeosporioides Gene 2018 670 63 69 10.1016/j.gene.2018.05.080 29792948 · DOI
  3. [3]Wu J. Ji Z. Wang N. Chi F. Xu C. Zhou Z. Zhang J. Identification of conidiogenesis-associated genes in Colletotrichum gloeosporioides by Agrobacterium tumefaciens-mediated transformation Curr. Microbiol. 2016 73 802 810 10.1007/s00284-016-1131-8 27582094 · DOI
  4. [4]Barhoom S. Sharon A. cAMP regulation of “pathogenic” and “saprophytic” fungal spore germination Fungal Genet. Biol. 2004 41 317 326 10.1016/j.fgb.2003.11.011 14761792 · DOI
  5. [5]Pereira I.S. Abreu M.S. Alves E. Ferreira J.B. Histopathological studies of the interaction Colletotrichum gloeosporioides: Coffee tree Bragantia 2009 68 117 123 10.1590/S0006-87052009000100013 · DOI
  6. [8]Wang Z.Y. Jenkinson J.M. Holcombe L.J. Soanes D.M. Veneault-Fourrey C. Bhambra G.K. Talbot N.J. The molecular biology of appressorium turgor generation by the rice blast fungus Magnaporthe grisea Biochem. Soc. Trans. 2005 33 384 388 10.1042/BST0330384 15787612 · DOI
  7. [9]Ryder L.S. Talbot N.J. Regulation of appressorium development in pathogenic fungi Curr. Opin. Plant Biol. 2015 26 8 13 10.1016/j.pbi.2015.05.013 26043436 PMC4781897 · DOI
  8. [10]He P.H.Z. Wang Y.L. Wang X.L. Zhang X. Tian C. The mitogen-activated protein kinase CgMK1 governs appressorium formation, melanin synthesis, and plant infection of Colletotrichum gloeosporioides Front. Microbiol. 2017 8 2216 10.3389/fmicb.2017.02216 29176970 PMC5686099 · DOI
  9. [12]Fang Y.L. Xia L.M. Wang P. Zhu L.-H. Ye J.-R. Huang L. The MAPKKK CgMck1 is required for cell wall integrity, appressorium development, and pathogenicity in Colletotrichum gloeosporioides Genes 2018 9 543 10.3390/genes9110543 30413120 PMC6267176 · DOI
  10. [13]Wang P. Li B. Pan Y.T. Zhang Y.Z. Li D.W. Huang L. Calcineurin-responsive transcription factor CgCrzA is required for cell wall integrity and infection-related morphogenesis in Colletotrichum gloeosporioides Plant Pathol. J. 2020 36 385 397 10.5423/PPJ.OA.04.2020.0071 33082723 PMC7542025 · DOI
  11. [14]Liu N. Meng F.L. Tian C.M. Transcriptional network in Colletotrichum gloeosporioides mutants lacking Msb2 or Msb2 and Sho1 J. Fungi 2022 8 207 10.3390/jof8020207 PMC8878819 35205961 · DOI
  12. [15]Li Y.F. He P.H.Z. Tian C.M. Wang Y. CgHog1 controls the adaptation to both sorbitol and fludioxonil in Colletotrichum gloeosporioides Fungal Genet. Biol. 2020 135 103289 10.1016/j.fgb.2019.103289 31704368 · DOI
  13. [16]Wang X.L. Lu D.X. Tian C.M. Analysis of melanin biosynthesis in the plant pathogenic fungus Colletotrichum gloeosporioides Fungal Biol. 2021 125 679 692 10.1016/j.funbio.2021.04.004 34420695 · DOI · Paper page
  14. [17]Henson J.M. Butler M.J. Day A.W. The dark side of the mycelium: Melanins of phytopathogenic fungi Annu. Rev. Phytopathol. 1999 37 447 471 10.1146/annurev.phyto.37.1.447 11701831 · DOI
  15. [18]Zhang T. Zhang B. Hua C. Meng P. Wang S. Chen Z. Du Y. Gao F. Huang J. VdPKS1 is required for melanin formation and virulence in a cotton wilt pathogen Verticillium dahliae Sci. China Life Sci. 2017 60 868 879 10.1007/s11427-017-9075-3 28755294 · DOI
  16. [19]Schumacher J. DHN melanin biosynthesis in the plant pathogenic fungus Botrytis cinerea is based on two developmentally regulated key enzyme (PKS)-encoding genes Mol. Microbiol. 2016 99 729 748 10.1111/mmi.13262 26514268 · DOI · Paper page
  17. [20]Scindiya M. Malathi P. Kaverinathan K. Sundar A.R. Viswanathan R. RNA-mediated silencing of PKS1 gene in Colletotrichum falcatum causing red rot in sugarcane Eur. J. Plant Pathol. 2019 153 371 384 10.1007/s10658-018-1563-z · DOI
  18. [21]Takano Y. Kubo Y. Shimizu K. Mise K. Okuno T. Furusawa I. Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis in Colletotrichum lagenarium Mol. Gen. Genet. 1995 249 162 167 10.1007/BF00290362 7500937 · DOI
  19. [22]Tsuji G. Kenmochi Y. Takano Y. Sweigard J. Farrall L. Furusawa I. Horino O. Kubo Y. Novel fungal transcriptional activators, Cmr1p of Colletotrichum lagenarium and Pig1p of Magnaporthe grisea, contain Cys2His2 zinc finger and Zn (II) 2Cys6 binuclear cluster DNA-binding motifs and regulate transcription of melanin biosynthesis genes in a developmentally specific manner Mol. Microbiol. 2000 38 940 954 11123670
  20. [23]Wang Y. Hu X. Fang Y. Anchieta A. Goldman P.H. Hernandez G. Klosterman S.J. Transcription factor VdCmr1 is required for pigment production, protection from UV irradiation, and regulates expression of melanin biosynthetic genes in Verticillium dahliae Microbiology 2018 164 685 10.1099/mic.0.000633 29485393 PMC5982140 · DOI · Paper page
  21. [24]Nishimura M. Fukada J. Moriwaki A. Fujikawa T. Ohashi M. Hibi T. Hayashi N. Mstu1, an APSES transcription factor, is required for appressorium-mediated infection in Magnaporthe grisea Biosci. Biotechnol. Biochem. 2009 73 1779 1786 10.1271/bbb.90146 19661696 · DOI
  22. [25]Cao H.J. Huang P.Y. Yan Y.X. Shi Y. Dong B. Liu X. Ye L. Lin F. Lu J. The basic helix-loop-helix transcription factor Crf1 is required for development and pathogenicity of the rice blast fungus by regulating carbohydrate and lipid metabolism Environ. Microbiol. 2018 20 3427 3441 10.1111/1462-2920.14387 30126031 · DOI
  23. [26]Thines E. Weber R.W. Talbot N.J. MAP kinase and protein kinase A-dependent mobilization of triacylglycerol and glycogen during appressorium turgor generation by Magnaporthe grisea Plant Cell 2000 12 1703 1718 11006342 PMC149080
  24. [27]Wilson R. Talbot N. Under pressure: Investigating the biology of plant infection by Magnaporthe oryzae Nat. Rev. Microbiol. 2009 7 185 195 10.1038/nrmicro2032 19219052 · DOI
  25. [28]Liu X.H. Liang S. Wei Y.Y. Zhu X.M. Li L. Liu P.P. Zheng Q.X. Zhou H.N. Zhang Y. Mao L.J. Metabolomics analysis identifies sphingolipids as key signaling moieties in appressorium morphogenesis and function in Magnaporthe oryzae mBio 2019 10 e01467-19 10.1128/mBio.01467-19 31431550 PMC6703424 · DOI
  26. [29]Ferreira R.B. Monteiro S. Freitas R. Santos C.N. Chen Z. Batista L.M. Duarte J. Borges A. Teixeira A.R. Fungal pathogens: The battle for plant infection Crit. Rev. Plant Sci. 2006 25 505 524 10.1080/07352680601054610 · DOI
  27. [30]Kim D. Langmead B. Salzberg S.L. HISAT: A fast spliced aligner with low memory requirements Nat. Methods 2015 12 357 360 10.1038/nmeth.3317 25751142 PMC4655817 · DOI · Paper page
  28. [31]Pertea M. Pertea G.M. Antonescu C.M. Chang T.C. Mendell J.T. Salzberg S.L. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads Nat. Biotechnol. 2015 33 290 295 10.1038/nbt.3122 25690850 PMC4643835 · DOI
  29. [32]Wang L. Feng Z. Wang X. Wang X. Zhang X. DEGseq: An R package for identifying differentially expressed genes from RNA-seq data Bioinformatics 2010 26 136 138 10.1093/bioinformatics/btp612 19855105 · DOI · Paper page
  30. [33]Storey J.D. Tibshirani R. Statistical significance for genomewide studies Proc. Natl. Acad. Sci. USA 2003 100 9440 9445 10.1073/pnas.1530509100 12883005 PMC170937 · DOI · Paper page
  31. [34]Conesa A. Götz S. Blast2GO: A comprehensive suite for functional analysis in plant genomics Int. J. Plant Genom. 2008 2008 619832 10.1155/2008/619832 18483572 PMC2375974 · DOI
  32. [35]Conesa A. Götz S. García-Gómez J.M. Terol J. Talón M. Robles M. Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research Bioinformatics 2005 21 3674 3676 10.1093/bioinformatics/bti610 16081474 · DOI
  33. [36]Ye J. Fang L. Zheng H. Zhang Y. Chen J. Zhang Z. Wang J. Li S. Li R. Bolund L. WEGO: A web tool for plotting GO annotations Nucleic Acids Res. 2006 34 293 297 10.1093/nar/gkl031 PMC1538768 16845012 · DOI
  34. [37]Su Y.L. Li J.M. Li M. Luan J.B. Ye X.D. Wang X.W. Liu S.S. Transcriptomic analysis of the salivary glands of an invasive whitefly PLoS ONE 2012 7 e39303 10.1371/journal.pone.0039303 22745728 PMC3379992 · DOI
  35. [38]Yin Y. Martin J. Abubucker S. Scott A.L. McCarter J.P. Wilson R.K. Jasmer D.P. Mitreva M. Intestinal transcriptomes of nematodes: Comparison of the parasites Ascaris suum and Haemonchus contortus with the free-living Caenorhabditis elegans PLoS Negl. Trop. Dis. 2008 2 e269 10.1371/journal.pntd.0000269 18682827 PMC2483350 · DOI
  36. [39]Li Z. Liang Y.M. Tian C.M. Characterization of the causal agent of poplar anthracnose occurring in the Beijing region Mycotax 2012 120 277 286 10.5248/120.277 · DOI
  37. [40]Livak K.J. Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method Methods 2001 25 402 408 10.1006/meth.2001.1262 11846609 · DOI · Paper page

Cited by

Sorted by citations

Authors
Qin, X., Tian, C., & Meng, F.
Affiliations
Beijing Forestry University (CN)
Corresponding author
Meng, Fanli
ORCID
Venue
International Journal of Molecular Sciences
Publisher
MDPI AG
Published
2023-04-18
Volume
24
Issue
8
Pages
7411
ISSN
1422-0067 (ISSN-L)
DOI
10.3390/ijms24087411
PMC
PMC10138971
OpenAlex
W4366290786
Cited by
10
References
0
Citations by year
2026: 4 · 2025: 4 · 2024: 1 · 2023: 1
FWCI
1.40
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Molecular Sciences (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (32201564, S202110022014)
  • Beijing Undergraduate Training Programs for Innovation and Entrepreneurship (32201564, S202110022014)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Plant Pathogens and Fungal Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
Keywords
Colletotrichum gloeosporioides, appressoria, turgor pressure, melanogenesis, fatty acid metabolism, biotin metabolism, fungal virulence genes
MeSH terms
Fatty Acids, Melanins, Plant Diseases, Colletotrichum, Gene Expression Profiling, Transcriptome

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