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Cited by 15biodegradable polymer synthesis and propertiesBone Tissue Engineering MaterialsElectrospun Nanofibers in Biomedical Applications

Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

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Esmail, Asiyah, Pereira, João R., Sevrin, Chantal, Grandfils, Christian, Menda, Ugur Deneb, Fortunato, Elvira, Oliva, Abel, Freitas, Filomena·2021·Life
DOI 10.3390/life11090935PMC PMC8466055
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Abstract

Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer’s polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27–49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18–3.35 MPa tensile strength and 0.07–0.11 MPa Young’s Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.

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  • The pore size of PHB-based scaffolds is a crucial parameter that highly affects cell attachment, proliferation, and differentiation.

    “The pore size of PHB-based scaffolds is another crucial parameter that highly affects cell attachment, proliferation, and differentiation [95].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [95]

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Authors
Esmail, A., Pereira, J. R., Sevrin, C., Grandfils, C., Menda, U. D., Fortunato, E., Oliva, A., & Freitas, F.
Affiliations
  • Instituto de Biologia Experimental e Tecnológica (PT)
  • Unidade em Ciências Biomoleculares Aplicadas (PT)
  • University of Liège (BE)
  • CENIMAT/i3N, Department of Materials Science, Nova School of Sciences and Technology, Nova University Lisbon, 2819-516 Caparica, Portugal
  • Centro de Investigação em Materiais (PT)
Corresponding author
Freitas, Filomena
ORCID
Venue
Life
Publisher
MDPI AG
Published
2021-09-08
Volume
11
Issue
9
Pages
935
ISSN
2075-1729 (ISSN-L)
DOI
10.3390/life11090935
PMC
PMC8466055
OpenAlex
W3198893939
Cited by
15
References
0
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 5 · 2022: 1
FWCI
0.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Life (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Open Repository and Bibliography (University of Liège) (submittedVersion)
Funders
Fundação para a Ciência e a Tecnologia (UIDP/04378/2020, UIDB/04378/2020, LA/P/0140/2020, iNOVA4Health/MULTI/04462/2018, UIDB/50025/2020, SFRH/BD/147518/2019)
Topics
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Keywords
polyhydroxybutyrate, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), polyhydroxyalkanoates, porous scaffolds, tissue engineering scaffolds, emulsion templating, hydrophilicity, water swelling, porosity, mechanical properties, crystallinity, biodegradability, biopolyesters

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