Cited by 17Metabolomics and Mass Spectrometry StudiesCancer and biochemical researchCancer, Hypoxia, and Metabolism

Functional metabolomics uncovers metabolic alterations associated to severe oxidative stress in MCF7 breast cancer cells exposed to ascididemin

Open access
Morvan, Daniel·2013·Marine Drugs

Abstract

Marine natural products are a source of promising agents for cancer treatment. However, there is a need to improve the evaluation of their mechanism of action in tumors. Metabolomics of the response to anti-tumor agents is a tool to reveal candidate biomarkers and metabolic targets. We used two-dimensional high-resolution magic angle spinning proton-NMR spectroscopy-based metabolomics to investigate the response of MCF7 breast cancer cells to ascididemin, a marine alkaloid and lead molecule for anti-cancer treatment. Ascididemin induced severe oxidative stress and apoptosis within 48 h of exposure. Thirty-three metabolites were quantified. Metabolic response involved downregulation of glycolysis and the tricarboxylic acid cycle, and phospholipid metabolism alterations. Candidate metabolic biomarkers of the response of breast cancer cells to ascididemin were proposed including citrate, gluconate, polyunsaturated fatty acids, glycerophospho-choline and -ethanolamine. In addition, candidate metabolic targets were identified. Overall, the response to Asc could be related to severe oxidative stress and anti-inflammatory effects.

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Authors
Morvan, D.
Affiliations
Université Clermont Auvergne (FR)
Corresponding author
Morvan, Daniel
Venue
Marine Drugs
Publisher
MDPI AG
Published
2013-10-11
Volume
11
Issue
10
Pages
3846-3860
ISSN
1660-3397 (ISSN-L)
OpenAlex
W2026158028
Cited by
17
References
18
Citations by year
2026: 1 · 2024: 1 · 2022: 2 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 1
FWCI
0.75
Type
journal article
Language
en
Open access
Locations
  • Marine Drugs (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer and biochemical research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
Keywords
marine alkaloids, breast cancer, MCF-7 cells, oxidative stress, apoptosis, TCA cycle, phospholipid metabolism, metabolic biomarkers, metabolic targets, anti-inflammatory activity, anticancer mechanism
MeSH terms
Alkaloids, Antineoplastic Agents, Breast Neoplasms, Female, Humans, Phenanthrolines, Quinolines, Oxidative Stress, Cell Line, Tumor, Metabolomics, Metabolome, MCF-7 Cells

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