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Cited by 287Bone Tissue Engineering MaterialsTissue Engineering and Regenerative MedicineHydrogels: synthesis, properties, applications

Chitosan-alginate biocomposite containing fucoidan for bone tissue engineering

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Venkatesan, Jayachandran, Bhatnagar, Ira, Kim, Se-Kwon·2014·Marine Drugs
DOI 10.3390/md12010300PMC PMC3917275
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Abstract

Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and retention ability of the prepared scaffolds showed similar efficacy. The pore size of the Chi-Alg and Chi-Alg-fucoidan scaffolds were measured from scanning electron microscopy and found to be 62–490 and 56–437 µm, respectively. In vitro studies using the MG-63 cell line revealed profound cytocompatibility, increased cell proliferation and enhanced alkaline phosphatase secretion in the Chi-Alg-fucoidan scaffold compared to the Chi-Alg scaffold. Further, protein adsorption and mineralization were about two times greater in the Chi-Alg-fucoidan scaffold than the Chi-Alg scaffold. Hence, we suggest that Chi-Alg-fucoidan will be a promising biomaterial for bone tissue regeneration.

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Sorted by citations

Authors
Venkatesan, J., Bhatnagar, I., & Kim, S.-K.
Affiliations
  • Pukyong National University (KR)
  • Centre for Cellular and Molecular Biology (IN)
Corresponding author
Kim, Se-Kwon
ORCID
Venue
Marine Drugs
Publisher
MDPI AG
Published
2014-01-16
Volume
12
Issue
1
Pages
300-316
ISSN
1660-3397 (ISSN-L)
DOI
10.3390/md12010300
PMC
PMC3917275
OpenAlex
W2015097479
Cited by
287
References
1
Citations by year
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FWCI
8.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Marine Drugs (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
fucoidan, bone tissue engineering, bone scaffolds, freeze-drying, bone graft substitutes, cytocompatibility, mineralization, protein adsorption, porosity, MG-63 cells, bone regeneration
MeSH terms
Adsorption, Alginates, Alkaline Phosphatase, Biocompatible Materials, Bone Regeneration, Calcification, Physiologic, Cell Line, Freeze Drying, Humans, Materials Testing, Microscopy, Electron, Scanning, Polysaccharides, Water, Porosity, Spectroscopy, Fourier Transform Infrared, Bone Substitutes, Tissue Engineering, Cell Line, Tumor, Chitosan, Cell Proliferation

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