Nrf2 and NF-κB Signaling Pathways Contribute to Porphyra-334-Mediated Inhibition of UVA-Induced Inflammation in Skin Fibroblasts
Open accessAbstract
In this study, we examined the protective effects of porphyra-334 against UVA-irradiated cellular damage and elucidated the underlying mechanisms. Porphyra-334 prevented UVA-induced cell death and exhibited scavenging activities against intracellular oxidative stress induced by UVA irradiation in skin fibroblasts. We found that porphyra-334 significantly reduced the secretion and expression of IL-6 and TNF-α, reduced nuclear expression of Nuclear factor-κB (NF-κB), and sustained NF-E2-related factor 2 (Nrf2) activation. Further mechanism research revealed that porphyra-334 promoted the Nrf2 signaling pathway in UVA-irradiated skin fibroblasts. Our results show that the antioxidant effect of porphyra-334 is due to the direct scavenging of oxidative stress and its inhibitory effects on NF-κB-dependent inflammatory genes, such as IL-6 and TNF-κ. Therefore, we hypothesize that boosting the Nrf2- NF-κB-dependent response to counteract environmental stress is a promising strategy for the prevention of UVA-related damage.
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Porphyra-334 demonstrated potent Nrf2 activation through prevention of UVA induced markers of inflammation and cell death; in skin fibroblasts incubated with 0-40 μM after UVA irradiation, it significantly reduced gene and protein expression of IL-6, IL-1β, TNF-α and nuclear NF-κB, sustained Nrf2 activation, increased expression of HMOX-1, GSH and NQO1, and directly scavenged reactive oxidative实体.
“This in silico approach has been confirmed experimentally with porphyra-334, which demonstrated potent Nrf2 activation activity through the prevention of UVA induced markers of inflammation and cell death. Skin fibroblasts were incubated with increasing concentrations of porphyra-334 (0-40μM) after UVA irradiation. This resulted in a significant reduction of gene and protein expression of IL-6, IL”
From Mycosporine-Like Amino Acids for Skin Photoprotection (2019) · cites this paper as [104]
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- Authors
- Ryu, J., Kwon, M.-J., & Nam, T.-J.
- Affiliations
- Pukyong National University (KR)
- Department of Food and Life Science, Pukyong National University, Busan 608-737, Korea
- Corresponding author
- Nam, Taek-Jeong
- ORCID
- Nam, Taek-Jeong
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2015-07-31
- Volume
- 13
- Issue
- 8
- Pages
- 4721-4732
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC4557001
- OpenAlex
- W1771980067
- Cited by
- 39
- References
- 23
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 10 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 5 · 2016: 4
- FWCI
- 4.47
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Research Foundation
- National Research Foundation of Korea (2012R1A6A1028677)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- porphyra-334, UVA irradiation, dermal fibroblasts, Nrf2 signaling, NF-κB signaling, oxidative stress, inflammation, interleukin-6, TNF-α, antioxidant activity, cell death, photoprotection
- MeSH terms
- Antioxidants, Cells, Cultured, Cyclohexanones, Fibroblasts, Glycine, Humans, Inflammation, Skin, Tumor Necrosis Factor-alpha, Ultraviolet Rays, Signal Transduction, Interleukin-6, NF-kappa B, Oxidative Stress, NF-E2-Related Factor 2
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