Food Is Reservoir of MDR Salmonella: Prevalence of ESBLs Profiles and Resistance Genes in Strains Isolated from Food
Open accessAbstract
Salmonella spp. are among the most frequent causes of foodborne diseases, and the increasing occurrence of MDR strains is an additional cause for concern. In the three-year period 2019–2021, we collected Salmonella spp. strains isolated from different food categories analysed in the context of Regulation (EC) No 2073/2005 in order to assess their antibiotic susceptibility profiles and ESBL production. To determine the susceptibility profiles and identify MDR strains, we used the Kirby–Bauer method to test 17 antibiotics. Double-disc and PCR testing then allowed us to assess the production of ESBLs and the presence of beta-lactamase resistance genes. Phenotypic tests showed that 36 out of 67 strains were MDR and 52.7% of these were ESBL producers. Finally, molecular investigations conducted on ESBL-producing strains revealed the presence of blaSHV, blaCTX-M and blaTEM genes. Our results confirmed the prevalence of S. Infantis, an MDR strain and ESBL producer, in chicken meat. This suggests that further research on the prevalence of antibiotic resistance genes (ARGs) in foodborne strains is needed, especially from a One Health perspective.
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Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products.
“Studies have revealed the presence of MDR bacteria, including methicillin-resistant S. aureus (MRSA), ESBL-producing Enterobacteriaceae, and MDR-Salmonella, in meat products [63,76,77,78,79].”
From A Systematic Review on Antimicrobial Resistance in Ghana from a One Health Perspective (2024) · cites this paper as [77]
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- Authors
- Gambino, D., Gargano, V., Butera, G., Sciortino, S., Pizzo, M., Oliveri, G., Cardamone, C., Piraino, C., Cassata, G., Vicari, D., & Costa, A.
- Affiliations
- Istituto Zooprofilattico Sperimentale Della Sicilia (IT)
- Corresponding author
- Gargano, Valeria
- ORCID
- Venue
- Microorganisms
- Publisher
- MDPI AG
- Published
- 2022-04-06
- Volume
- 10
- Issue
- 4
- Pages
- 780
- ISSN
- 2076-2607 (ISSN-L)
- PMC
- PMC9026803
- OpenAlex
- W4224271514
- Cited by
- 28
- References
- 26
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 11 · 2023: 8 · 2022: 1
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Microorganisms (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
- Keywords
- Salmonella, multidrug resistance, extended-spectrum beta-lactamase, antibiotic resistance genes, foodborne pathogens, chicken meat, Salmonella Infantis, blaSHV, blaCTX-M, blaTEM, food safety, One Health
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