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Cited by 13Ginseng Biological Effects and ApplicationsSkin Protection and AgingProteoglycans and glycosaminoglycans research

Ginseng Gintonin Enhances Hyaluronic Acid and Collagen Release from Human Dermal Fibroblasts Through Lysophosphatidic Acid Receptor Interaction

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Lee, Rami, Lee, Na-Eun, Hwang, Hongik, Rhim, Hyewhon, Cho, Ik-Hyun, Nah, Seung-Yeol·2019·Molecules
DOI 10.3390/molecules24244438PMC PMC6943507
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Abstract

Gintonin is a newly discovered component of ginseng and acts as a ligand for G protein-coupled lysophosphatidic acid (LPA) receptors. It is currently unclear whether gintonin has skin-related effects. Here, we examined the effects of a gintonin-enriched fraction (GEF) on [Ca2+]i transient induction in human dermal fibroblasts (HDFs). We found that GEF treatment transiently induced [Ca2+]i in a dose-dependent manner. GEF also increased cell viability and proliferation, which could be blocked by Ki16425, an LPA1/3 receptor antagonist, or 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), a calcium chelator. We further found that GEF stimulated hyaluronic acid (HA) release from HDFs in a dose- and time-dependent manner, which could be attenuated by Ki16425, U73122, a phospholipase C inhibitor, 2-Aminoethoxydiphenyl borate (2-APB), an IP3 receptor antagonist, and BAPTA-AM. Moreover, we found that GEF increased HA synthase 1 (HAS1) expression in a time-dependent manner. We also found that GEF stimulates collagen release and the expression of collagen 1, 3, and 7 synthases in a time-dependent manner. GEF-mediated collagen synthesis could be blocked by Ki16425, U73122, 2-APB, and BAPTA-AM. GEF treatment also increased the mRNA levels of LPA1-6 receptor subtypes at 8 h and increased the protein levels of LPA1-6 receptor subtypes at 8 h. Overall, these results indicate that the GEF-mediated transient induction of [Ca2+]i is coupled to HA and collagen release from HDFs via LPA receptor regulations. We can, thus, conclude that GEF might exert a beneficial effect on human skin physiology via LPA receptors.

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  • P. ginseng exerts protective effects against skin aging by reducing inflammation and stimulating collagen synthesis, as demonstrated in in vitro and in vivo models.

    “Numerous pharmacological studies have confirmed that P. ginseng exerts protective effects against skin aging by mechanisms such as reducing inflammation and stimulating collagen synthesis, as demonstrated through both in vitro and in vivo experimental models [10,11,12,13,14].”

    From Anti-Aging and Anti-Inflammatory Effects of Compounds from Fresh Panax ginseng Roots: A Study on TNF-α/IFN-γ-Induced Skin Cell Damage (2024) · cites this paper as [11]

    The Results (section 2.4; page unavailable) show that a ginseng-derived gintonin-enriched fraction increased collagen release and collagen-related gene expression in cultured human dermal fibroblasts. The Discussion (section 3; page unavailable) describes anti-aging effects as a possibility, not a demonstrated outcome. This paper does not report reduced skin inflammation or an in vivo skin-aging model. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Lee, R., Lee, N.-E., Hwang, H., Rhim, H., Cho, I.-H., & Nah, S.-Y.
Affiliations
  • Konkuk University (KR)
  • Korea Institute of Science and Technology (KR)
  • Kyung Hee University (KR)
Corresponding author
Nah, Seung-Yeol
ORCID
Venue
Molecules
Publisher
MDPI AG
Published
2019-12-04
Volume
24
Issue
24
Pages
4438
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules24244438
PMC
PMC6943507
OpenAlex
W2994043286
Cited by
13
References
0
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 2
FWCI
0.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • NRF of Korea funded by the Ministry of Science, ICT, and Future Planning for the Basic Science Research Program (NRF 2017R1D1A1A09000520)
  • The Brain Research Program (NRF2016M3C7A1913845)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ginseng Biological Effects and Applications
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
Keywords
gintonin, ginseng, lysophosphatidic acid receptor, human dermal fibroblasts, calcium transients, phospholipase C, IP3 receptor, HAS1, skin physiology, Ki16425
MeSH terms
Hyaluronan Synthases, Calcium, Cell Survival, Collagen, Fibroblasts, Panax, Humans, Hyaluronic Acid, Plant Extracts, Dermis, Cell Proliferation, Receptors, Lysophosphatidic Acid

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