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Cited by 11Fungal Biology and ApplicationsPolysaccharides Composition and ApplicationsAntifungal resistance and susceptibility

Solid-State 13C Nuclear Magnetic Resonance Study of Soluble and Insoluble β-Glucans Extracted from Candida lusitaniae

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Bikmurzin, Ruslan, Maršalka, Arūnas, Kalėdienė, Lilija·2023·Molecules
DOI 10.3390/molecules28248066PMC PMC10745363
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Abstract

β-glucans are widely known for their biological activities. However, the choice of extraction method can significantly influence their structural characteristics, thereby potentially impacting their biological functions. In this paper, three fractions of β-glucans were obtained from Candida lusitaniae yeast via alkali and hot-water extraction methods and were analyzed using solid-state 13C nuclear magnetic resonance (NMR) spectroscopy. Solid-state NMR spectroscopy was used as a nondestructive technique that preserves the structure of the analyzed molecules. The results suggest that differences in the β-glucan structure are affected by the choice of extraction method. The main difference occurred in the 82–92 ppm region with signal presence suggesting that β-glucans have a linear structure when hot-water-extracted, which is absent in alkali-extracted fractions resulting in the acquisition of β-glucans with an ordered, possibly helical structure. A hot-water extracted water-insoluble (HWN) fraction consists of linear β-1,3-glucans with other signals indicating the presence of β-1,6-linked side chains, chitin and small amounts of α-glucan impurities. For those that are alkali-extracted, alkali-insoluble (AN) and water-soluble (AWS) fractions are structurally similar and consist of an ordered β-1,3-glucan structure with β-1,6-linked side chains and a significant amount of α-glucan and chitin in both fractions.

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  • C. albicans cell walls share a relatively simple structure, with the rigid structural framework containing only chitin microfibrils and stiff linear β-1,3-glucan backbones, which may primarily adopt the triple helix conformation, within the inner layer.

    “These findings demonstrate that C. albicans cell walls share a relatively simple structure, with the rigid structural framework containing only chitin microfibrils and stiff linear β-1,3-glucan backbones, which may primarily adopt the triple helix conformation67–69, within the inner layer.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [69]

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  • Among these two forms, the former corresponds to type-a β-1,3-glucan observed at room-temperature, representing the bulk β-1,3-glucans in the model triple-helix configuration, exhibiting no interaction with chitin.

    “Among these two forms, the former corresponds to type-a β-1,3-glucan observed at room-temperature (Fig. 2e), representing the bulk β-1,3-glucans in the model triple-helix configuration67–69, exhibiting no interaction with chitin.”

    From Distinct echinocandin responses of Candida albicans and Candida auris cell walls revealed by solid-state NMR (2025) · cites this paper as [69]

    The Results (page not available) suggest that β-1,3-glucans in the alkali-extracted fractions may have an ordered backbone, possibly a triple helix, but also offer a double helix as an alternative. The paper does not identify this as room-temperature type-a glucan or establish that it represents the bulk β-1,3-glucan. Its Results also describe possible β-1,3-glucan–chitin linkages, rather than establishing no interaction with chitin. (LLM, conf=0.86)

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Sorted by citations

Authors
Bikmurzin, R., Maršalka, A., & Kalėdienė, L.
Affiliations
  • Vilnius University (LT)
  • Nature Research Centre (LT)
Corresponding author
Bikmurzin, Ruslan
ORCID
Bikmurzin, Ruslan
Venue
Molecules
Publisher
MDPI AG
Published
2023-12-13
Volume
28
Issue
24
Pages
8066
ISSN
1420-3049 (ISSN-L)
DOI
10.3390/molecules28248066
PMC
PMC10745363
OpenAlex
W4389670810
Cited by
11
References
0
Citations by year
2026: 2 · 2025: 6 · 2024: 3
FWCI
1.71
Type
journal article
Language
en
Open access
Licence
Locations
  • Molecules (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Institutional Repository of Nature Research Centre (submittedVersion, other-oa)
  • Institutional Repository of Vilnius College of Technologies and Design (submittedVersion, other-oa)
Topics
  • Health Sciences › Medicine › Pharmacology › Fungal Biology and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
β-glucan, Candida lusitaniae, yeast cell wall, extraction methods, hot water extraction, alkaline extraction, β-1,3-glucan, chitin, helical conformation, glucan structure
MeSH terms
Alkalies, Chitin, Saccharomycetales, Glucans, Magnetic Resonance Spectroscopy, Water, beta-Glucans

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