Therapeutic Effects of Atranorin towards the Proliferation of Babesia and Theileria Parasites
Open accessAbstract
Atranorin (ATR), is a compound with multidirectional biological activity under different in vitro and in vivo conditions and it is effective as an antibacterial, antiviral, antiprotozoal and anti-inflammatory agent. In the current study, the in vitro as well as in vivo chemotherapeutic effect of ATR as well as its combined efficacy with the existing antibabesial drugs (diminazene aceturate (DA), atovaquone (AV) and clofazimine (CF)) were investigated on six species of piroplasm parasites. ATR suppressed B. bovis, B. bigemina, B. divergens, B. caballi and T. equi multiplication in vitro with IC50 values of 98.4 ± 4.2, 64.5 ± 3.9, 45.2 ± 5.9, 46.6 ± 2.5, and 71.3 ± 2.7 µM, respectively. The CCK test was used to examine ATR’s cytotoxicity and adverse effects on different animal and human cell lines, the main hosts of piroplasm parasites and it showed that ATR affected human foreskin fibroblasts (HFF), mouse embryonic fibroblast (NIH/3T3) and Madin-Darby Bovine Kidney (MDBK) cell viability in a dose-related effect with a moderate selective index. The combined efficacy of ATR with DA, CF, and AV exhibited a synergistic and additive efficacy toward all tested species. In the in vivo experiment, ATR prohibited B. microti multiplication in mice by 68.17%. The ATR-DA and ATR-AV combination chemotherapies were more potent than ATR monotherapy. These results indicate the prospects of ATR as a drug candidate for piroplasmosis treatment.
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Garlic-artemether combined therapy may have a potential role in reducing organ injury and protecting against Plasmodium species by affecting NO production.
“They reported that this combined therapy may have a potential role in reducing organ injury and protecting against Plasmodium species by affecting NO production, suggesting novel treatment options against malaria [138,139].”
From Chemical Constituents and Pharmacological Activities of Garlic (Allium sativum L.): A Review (2020) · cites this paper as [139]
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- Authors
- Beshbishy, A. M., Batiha, G. E.-S., Alkazmi, L., Nadwa, E., Rashwan, E., Abdeen, A., Yokoyama, N., & Igarashi, I.
- Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine (JP)
- Damanhour University (EG)
- Umm al-Qura University (SA)
- Cairo University (EG)
- Al-Azhar University (EG)
- Benha University (EG)
- Corresponding author
- Batiha, Gaber El-Saber
- ORCID
- Venue
- Pathogens
- Publisher
- MDPI AG
- Published
- 2020-02-17
- Volume
- 9
- Issue
- 2
- Pages
- 127
- ISSN
- 2076-0817 (ISSN-L)
- PMC
- PMC7167990
- OpenAlex
- W3005703408
- Cited by
- 23
- References
- 39
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 12
- FWCI
- 3.19
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Pathogens (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Japan Society for the Promotion of Science (JSPS) (18H02337)
- Topics
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Health Sciences › Medicine › Epidemiology › Trypanosoma species research and implications
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Keywords
- atranorin, Babesia, Theileria, piroplasmosis, drug combination therapy, drug synergy, diminazene aceturate, atovaquone, clofazimine, Babesia bovis, Babesia bigemina, Babesia divergens, Babesia caballi, Babesia microti, Theileria equi, cytotoxicity, selectivity index, chemotherapy efficacy
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