The R2R3 MYB transcription factor MYB71 regulates abscisic acid response in arabidopsis
Open accessAbstract
Abscisic acid (ABA) regulates plant responses to abiotic stresses via regulating the expression of downstream genes, yet the functions of many ABA responsive genes remain unknown. We report here the characterization of MYB71, a R2R3 MYB transcription factor in regulating ABA responses in Arabidopsis. RT-PCR results show that the expression level of MYB71 was increased in response to ABA treatment. Arabidopsis protoplasts transfection results show that MYB71 was specifically localized in nucleus and it activated the Gal4:GUS reporter gene when recruited to the Gal4 promoter by a fused DNA binding domain GD. Roles of MYB71 in regulating plant response to ABA were analyzed by generating Arabidopsis transgenic plants overexpression MYB71 and gene edited mutants of MYB71. The results show that ABA sensitivity was increased in the transgenic plants overexpression MYB71, but decreased in the MYB71 mutants. By using a DEX inducible system, we further identified genes are likely regulated by MYB71, and found that they are enriched in biological process to environmental stimuli including abiotic stresses, suggesting that MYB71 may regulate plant response to abiotic stresses. Taken together, our results suggest that MYB71 is an ABA responsive gene, and MYB71 functions as a transcription activator and it positively regulates ABA response in Arabidopsis.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
CaAIEF1 enhances ABA sensitivity and drought tolerance in Capsicum annuum.
“transcription factors such as MYB37 and CaAIEF1 have been shown to enhance ABA sensitivity and drought tolerance in Arabidopsis and Capsicum annuum, respectively”
From Emerging applications of gene editing technologies for the development of climate-resilient crops (2025) · cites this paper as Cheng et al., 2022
The Introduction discusses ABA-responsive regulators and drought tolerance in Arabidopsis, but does not address CaAIEF1 in Capsicum annuum. Searches for CaAIEF1 and for pepper/Capsicum with drought returned no passage supporting the attributed claim. (LLM, conf=0.74)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014 · see citing works
MYB transcription factors in Arabidopsis
Dubos, C., Stracke, R., Erich Grotewold, A., Weißhaar, B., Martin, C. R., & Lepiniec, L. (2010) · Trends in Plant Science · doi:10.1016/j.tplants.2010.06.005 · Cited by 4,885 · see citing works
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis
Edwards, K. J., Johnstone, C., & Thompson, C. (1991) · Nucleic Acids Research · doi:10.1093/nar/19.6.1349 · Cited by 2,689 · see citing works
Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.04.007 · Cited by 2,369 · see citing works
Self-citationThe R2R3-MYB gene family in Arabidopsis thaliana
Stracke, R., Werber, M., & Weißhaar, B. (2001) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(00)00199-0 · Cited by 2,180 · see citing works
Gonzalez, A., Zhao, M., Leavitt, J. M., & Lloyd, A. M. (2007) · The Plant Journal · doi:10.1111/j.1365-313x.2007.03373.x · Cited by 1,904 · see citing works
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation
Hajdukiewicz, P. T. J., Sváb, Z., & Maliga, P. (1994) · Plant Molecular Biology · doi:10.1007/bf00014672 · Cited by 1,536 · see citing works
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
Borevitz, J., Xia, Y., Blount, J. W., Dixon, R. A., & Lamb, C. J. (2000) · The Plant Cell · doi:10.1105/tpc.12.12.2383 · Cited by 1,451 · see citing works
ABA-dependent and ABA-independent signaling in response to osmotic stress in plants
Yoshida, T., Mogami, J., & Yamaguchi‐Shinozaki, K. (2014) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2014.07.009 · Cited by 1,103 · see citing works
Wang, Z.-P., Xing, H.-L., Li, D., Zhang, H.-Y., Han, C.-Y., Wang, X.-C., & Chen, Q.-J. (2015) · Genome biology · doi:10.1186/s13059-015-0715-0 · Cited by 1,036 · see citing works
Self-citationThe Roles of Auxin Response Factor Domains in Auxin-Responsive Transcription
Tiwari, S. B., Hagen, G., & Guilfoyle, T. J. (2003) · The Plant Cell · doi:10.1105/tpc.008417 · Cited by 1,008 · see citing works
Zhong, R., Lee, C., Zhou, J., et al. (2008) · The Plant Cell · doi:10.1105/tpc.108.061325 · Cited by 962 · see citing works
Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport
Umezawa, T., Nakashima, K., Miyakawa, T., Kuromori, T., Tanokura, M., Shinozaki, K., & Yamaguchi‐Shinozaki, K. (2010) · Plant and Cell Physiology · doi:10.1093/pcp/pcq156 · Cited by 924 · see citing works
Fujii, H., & Zhu, J. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0903144106 · Cited by 885 · see citing works
Arabidopsis Basic Leucine Zipper Proteins That Mediate Stress-Responsive Abscisic Acid Signaling
Kang, J.-Y., Choi, H.-I., Im, M.-Y., et al. (2002) · The Plant Cell · doi:10.1105/tpc.010362 · Cited by 845 · see citing works
Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Byrne, M. E., Barley, R., Curtis, M. D., Arroyo, J. M., Dunham, M. J., Hudson, A., & Martienssen, R. A. (2000) · Nature · doi:10.1038/35050091 · Cited by 816 · see citing works
Zhong, R., Demura, T., & Ye, Z. (2006) · The Plant Cell · doi:10.1105/tpc.106.047399 · Cited by 757 · see citing works
Jung, C., Seo, J. S., Han, S. W., Koo, Y. J., Kim, C. H., Song, S. I., Nahm, B. H., Choi, Y. D., & Cheong, J. (2007) · PLANT PHYSIOLOGY · doi:10.1104/pp.107.110981 · Cited by 748 · see citing works
Fujii, H., Verslues, P. E., & Zhu, J. (2007) · The Plant Cell · doi:10.1105/tpc.106.048538 · Cited by 733 · see citing works
Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., et al. (2009) · Plant and Cell Physiology · doi:10.1093/pcp/pcp147 · Cited by 733 · see citing works
ABA receptor PYL9 promotes drought resistance and leaf senescence
Zhao, Y., Chan, Z., Gao, J., Lu, X., Cao, M., Yu, C., Hu, Y., You, J., Shi, H., Zhu, Y., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1522840113 · Cited by 700 · see citing works
WRKY transcription factors: key components in abscisic acid signalling
Rushton, D. L., Tripathi, P., Rabara, R., et al. (2011) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2011.00634.x · Cited by 693 · see citing works
ABA signal transduction at the crossroad of biotic and abiotic stress responses
Lee, S. C., & Luan, S. (2011) · Plant Cell & Environment · doi:10.1111/j.1365-3040.2011.02426.x · Cited by 693 · see citing works
Yoshida, T., Fujita, Y., Maruyama, K., et al. (2014) · Plant Cell & Environment · doi:10.1111/pce.12351 · Cited by 667 · see citing works
A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules
Oppenheimer, D. G., Herman, P. L., Sivakumaran, S., et al. (1991) · Cell · doi:10.1016/0092-8674(91)90523-2 · Cited by 657 · see citing works
ABI1 Protein Phosphatase 2C Is a Negative Regulator of Abscisic Acid Signaling
Gosti, F., Beaudoin, N., Serizet, C., et al. (1999) · The Plant Cell · doi:10.1105/tpc.11.10.1897 · Cited by 627 · see citing works
Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain
Tiwari, S. B., Hagen, G., & Guilfoyle, T. J. (2004) · The Plant Cell · doi:10.1105/tpc.017384 · Cited by 588 · see citing works
Lee, M. M., & Schiefelbein, J. (1999) · Cell · doi:10.1016/s0092-8674(00)81536-6 · Cited by 585 · see citing works
Global Analysis of Direct Targets of Secondary Wall NAC Master Switches in Arabidopsis
Zhong, R., Lee, C., & Ye, Z. (2010) · Molecular Plant · doi:10.1093/mp/ssq062 · Cited by 434 · see citing works
The Arabidopsis Transcription Factor MYB77 Modulates Auxin Signal Transduction
Shin, R., Burch, A. Y., Huppert, K. A., Tiwari, S. B., Murphy, A. S., Guilfoyle, T. J., & Schachtman, D. P. (2007) · The Plant Cell · doi:10.1105/tpc.107.050963 · Cited by 424 · see citing works
Arabidopsis MYB4 plays dual roles in flavonoid biosynthesis
Wang, X., Wu, J., Guan, M., et al. (2019) · The Plant Journal · doi:10.1111/tpj.14570 · Cited by 367 · see citing works
Self-citationThe heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses
Huang, Y.-C., Niu, C.-Y., Yang, C.-R., & Jinn, T.-L. (2016) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.00860 · Cited by 321 · see citing works
Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System
Lu, Y., Ye, X., Guo, R., Huang, J., Wang, W., Tang, J., Tan, L., Zhu, J., Chu, C., & Qian, Y. (2017) · Molecular Plant · doi:10.1016/j.molp.2017.06.007 · Cited by 306 · see citing works
Agrochemical control of plant water use using engineered abscisic acid receptors
Park, S., Peterson, F. C., Mosquna, A., Yao, J., Volkman, B. F., & Cutler, S. R. (2015) · Nature · doi:10.1038/nature14123 · Cited by 287 · see citing works
Xu, Z., Kim, S. Y., Hyeon, D. Y., Kim, D. H., Dong, T., Park, Y., Jin, J. B., Joo, S., Kim, S., Hong, J. C., et al. (2013) · The Plant Cell · doi:10.1105/tpc.113.119099 · Cited by 280 · see citing works
Abscisic acid: biosynthesis, inactivation, homoeostasis and signalling
Dong, T., Park, Y., & Hwang, I. (2015) · Essays in Biochemistry · doi:10.1042/bse0580029 · Cited by 275 · see citing works
Cuticular Wax Biosynthesis is Up-Regulated by the MYB94 Transcription Factor in Arabidopsis
Lee, S. B., & Suh, M. C. (2014) · Plant and Cell Physiology · doi:10.1093/pcp/pcu142 · Cited by 262 · see citing works
Liu, Y., Ji, X., Nie, X., Qu, M., Zheng, L., Tan, Z., Zhao, H., Huo, L., Liu, S., Zhang, B., et al. (2015) · New Phytologist · doi:10.1111/nph.13387 · Cited by 261 · see citing works
ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis
Rodrı́guez, P. L., Benning, G., & Grill, E. (1998) · FEBS Letters · doi:10.1016/s0014-5793(97)01558-5 · Cited by 252 · see citing works
Gao, X., Chen, J., Dai, X., Zhang, D., & Zhao, Y. (2016) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.00663 · Cited by 246 · see citing works
Cui, M. H., Yoo, K. S., Hyoung, S., Nguyen, H. T. K., Kim, Y. Y., Kim, H. J., Ok, S. H., Yoo, S. D., & Shin, J. S. (2013) · FEBS Letters · doi:10.1016/j.febslet.2013.04.028 · Cited by 242 · see citing works
Dekkers, B. J. W., Pearce, S. P., van Bolderen-Veldkamp, R. P., et al. (2013) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.223511 · Cited by 227 · see citing works
Zhang, P., Wang, R., Yang, X., Ju, Q., Li, W., Lü, S., Tran, L. P., & Xu, J. (2020) · Plant Cell & Environment · doi:10.1111/pce.13784 · Cited by 200 · see citing works
Wang, S., Tiwari, S. B., Hagen, G., et al. (2005) · The Plant Cell · doi:10.1105/tpc.105.031096 · Cited by 189 · see citing works
Self-citationWang, L., Ran, L., Hou, Y., Tian, Q., Li, C., Liu, R., Fan, D., & Luo, K. (2017) · New Phytologist · doi:10.1111/nph.14569 · Cited by 169 · see citing works
Kim, S. H., Lam, P. Y., Lee, M.-H., et al. (2020) · Frontiers in Plant Science · doi:10.3389/fpls.2020.583153 · Cited by 140 · see citing works
AtMYB21, a gene encoding a flower‐specific transcription factor, is regulated by COP1
Shin, B., Choi, G., Choi, G., et al. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01264.x · Cited by 133 · see citing works
Chen, S., Zhang, N., Zhou, G., Hussain, S., Ahmed, S., Tian, H., & Wang, S. (2021) · BMC Plant Biology · doi:10.1186/s12870-021-02907-9 · Cited by 109 · see citing works
Self-citationKim, J. H., Hyun, W. Y., Nguyen, N. H., et al. (2014) · Plant Cell & Environment · doi:10.1111/pce.12415 · Cited by 107 · see citing works
Gong, Q., Li, S., Zheng, Y., Duan, H., Xiao, F., Zhuang, Y.-F., He, J., Wu, G., Zhao, S., Zhou, H., et al. (2020) · The Plant Journal · doi:10.1111/tpj.14689 · Cited by 106 · see citing works
Abscisic Acid Receptors: Past, Present and FutureF
Guo, J., Yang, X., Weston, D. J., et al. (2011) · Journal of Integrative Plant Biology · doi:10.1111/j.1744-7909.2011.01044.x · Cited by 105 · see citing works
Wang, X., Wang, X., Hu, Q., et al. (2015) · The Plant Journal · doi:10.1111/tpj.12887 · Cited by 105 · see citing works
Self-citationYang, X., Li, J., Pei, M., et al. (2006) · Plant Cell Reports · doi:10.1007/s00299-006-0229-z · Cited by 95 · see citing works
Yu, Y., Wu, Z., Lü, K., Bi, C., Liang, S., Wang, X., & Zhang, D.-P. (2015) · Plant Molecular Biology · doi:10.1007/s11103-015-0411-1 · Cited by 84 · see citing works
Self-citationKnoth, C., & Eulgem, T. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03486.x · Cited by 81 · see citing works
A novel family of transcription factors conserved in angiosperms is required for ABA signalling
Tian, H., Chen, S., Yang, W., Wang, T., Zheng, K., Wang, Y., Cheng, Y., Zhang, N., Liu, S., Li, D., et al. (2017) · Plant Cell & Environment · doi:10.1111/pce.13058 · Cited by 81 · see citing works
Self-citationLiu, F., Jiang, H., Ye, S., et al. (2010) · Cell Research · doi:10.1038/cr.2010.36 · Cited by 78 · see citing works
Dai, X., Zhou, L., Zhang, W., et al. (2015) · Plant Cell & Environment · doi:10.1111/pce.12695 · Cited by 71 · see citing works
De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
He, W., Zhang, L., Villarreal, O. D., Fu, R., Bedford, E., Dou, J., Patel, A. Y., Bedford, M. T., Shi, X., Chen, T., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-12489-8 · Cited by 69 · see citing works
MYB70 modulates seed germination and root system development in Arabidopsis
Wan, J., Wang, R., Zhang, P., et al. (2021) · iScience · doi:10.1016/j.isci.2021.103228 · Cited by 65 · see citing works
Zheng, K., Wang, Y., & Wang, S. (2019) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2019.03.026 · Cited by 63 · see citing works
Self-citationLi, X., Zhong, M., Qu, L., et al. (2021) · Plant Science · doi:10.1016/j.plantsci.2021.110983 · Cited by 53 · see citing works
MYB76 Inhibits Seed Fatty Acid Accumulation in Arabidopsis
Duan, S., Jin, C., Li, D., et al. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.00226 · Cited by 48 · see citing works
Barco, B., Kim, Y., & Clay, N. K. (2019) · Nature Communications · doi:10.1038/s41467-019-11406-3 · Cited by 48 · see citing works
Deng, M.-D., Wang, Y., Kuzma, M., Chalifoux, M., Tremblay, L. J., Yang, S., Ying, J., Sample, A., Wang, H., Griffiths, R., et al. (2020) · The Plant Journal · doi:10.1111/tpj.15019 · Cited by 43 · see citing works
Jemmat, A. M., Ranocha, P., Ru, A. L., et al. (2020) · Plant Science · doi:10.1016/j.plantsci.2020.110565 · Cited by 39 · see citing works
Beathard, C., Mooney, S., Al‐Saharin, R., Goyer, A., & Hellmann, H. A. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.629208 · Cited by 33 · see citing works
Kim, Y. Y., Cui, M. H., Noh, M., et al. (2017) · Plant and Cell Physiology · doi:10.1093/pcp/pcx003 · Cited by 32 · see citing works
Involvement of ABA Responsive SVB Genes in the Regulation of Trichome Formation in Arabidopsis
Hussain, S., Zhang, N., Wang, W., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22136790 · Cited by 30 · see citing works
Self-citationYang, W., Chen, S., Cheng, Y., Zhang, N., Ma, Y., Wang, W., Tian, H., Li, Y., Hussain, S., & Wang, S. (2020) · Plant Signaling & Behavior · doi:10.1080/15592324.2020.1744293 · Cited by 27 · see citing works
Self-citationJia, T., Zhang, K., Li, F., Huang, Y., Fan, M., & Huang, T. (2020) · Plant Cell Reports · doi:10.1007/s00299-020-02602-3 · Cited by 26 · see citing works
An auxin responsive CLE gene regulates shoot apical meristem development in Arabidopsis
Guo, H., Zhang, W., Tian, H., Zheng, K., Dai, X., Liu, S., Hu, Q., Wang, X., Liu, B., & Wang, S. (2015) · Frontiers in Plant Science · doi:10.3389/fpls.2015.00295 · Cited by 24 · see citing works
Self-citationWang, W., Wang, X., Wang, Y., et al. (2020) · GM crops & food · doi:10.1080/21645698.2020.1790287 · Cited by 21 · see citing works
Self-citationAtMYB109 negatively regulates stomatal closure under osmotic stress in Arabidopsis thaliana
So, W. M., Huque, A. K. M. M., Shin, H.-Y., et al. (2020) · Journal of Plant Physiology · doi:10.1016/j.jplph.2020.153292 · Cited by 21 · see citing works
Evolution of AITR family genes in cotton and their functions in abiotic stress tolerance
Wang, T., Dong, Q., Wang, W., et al. (2020) · Plant Biology · doi:10.1111/plb.13218 · Cited by 15 · see citing works
Self-citationMa, Y., Tian, H., Lin, R., et al. (2021) · Scientific Reports · doi:10.1038/s41598-020-80695-2 · Cited by 13 · see citing works
Self-citationWang, X., Wang, W., Wang, Y., et al. (2020) · Journal of Plant Interactions · doi:10.1080/17429145.2020.1778110 · Cited by 11 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Emerging applications of gene editing technologies for the development of climate-resilient crops
Chavhan, R. L., Jaybhaye, S. G., Hinge, V. R., Deshmukh, A. S., Shaikh, U. S., Jadhav, P. K., Kadam, U. S., & Hong, J. C. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1524767 · Cited by 43 · see citing works
- Authors
- Cheng, Y., Ma, Y., Zhang, N., Lin, R., Yuan, Y., Tian, H., Hussain, S., Chen, S., Yang, W., Cai, L., Li, Y., Wang, X., Wang, T., & Wang, S.
- Affiliations
- Northeast Normal University (CN)
- Linyi University (CN)
- Corresponding author
- Wang, Shucai
- ORCID
- Venue
- Plants
- Publisher
- MDPI AG
- Published
- 2022-05-21
- Volume
- 11
- Issue
- 10
- Pages
- 1369
- ISSN
- 2223-7747 (ISSN-L)
- PMC
- PMC9143609
- OpenAlex
- W4281255172
- Cited by
- 25
- References
- 77
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 5 · 2023: 5 · 2022: 2
- FWCI
- 2.62
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plants (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Nature Science Foundation of China (32071938, LYDX2019BS039)
- Linyi University (32071938, LYDX2019BS039)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Seed Germination and Physiology
- Keywords
- R2R3-MYB transcription factor, abscisic acid response, ABA sensitivity, abiotic stress response, transcriptional activation, ABA-responsive genes, Arabidopsis thaliana
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
