A comparative study of dynamic light and x-ray scatterings on micelles of topological polymer amphiphiles
Open accessAbstract
Micelles were prepared in organic solvents by using three topological polymer amphiphiles: (i) cyclic poly(n-decyl glycidyl ether-block-2-(2-(2-methoxyethoxy)ethoxy)ethyl glycidyl ether) (c-PDGE-b-PTEGGE) and (ii) its linear analogue (l-PDGE-b-PTEGGE); (iii) linear poly(6-phosphorylcholinehexylthiopropyl glycidyl ether-block-n-dodecanoyl glycidyl ether) (l-PPCGE-b-PDDGE). For the individual micelle solutions, the size and distribution were determined by dynamic light scattering (DLS) and synchrotron X-ray scattering analyses. The synchrotron X-ray scattering analysis further found that c-PDGE-b-PTEGGE forms oblate ellipsoidal micelle in an ethanol/water mixture, l-PDGE-b-PTEGGE makes prolate ellipsoidal micelle in an ethanol/water mixture, and l-PPCGE-b-PDDGE forms cylindrical micelle in chloroform. This comparative study found that there are large differences in the size and distribution results extracted by DLS and X-ray scattering analyses. All possible factors to cause such large differences are discussed. Moreover, a better use of the DLS instrument with keeping its merits is proposed.
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DLS advantages are non-invasive measurement, minimal sample preparation, and avoidance of pre-experimental calibration.
“DLS advantages are the non-invasive measurement, the minimal sample preparation, and the avoidance of pre-experimental calibration [133].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [133]
The Introduction describes DLS measurement as easy, which broadly supports the claim of convenience. It does not state that DLS is non-invasive, requires minimal sample preparation, or avoids pre-experimental calibration; the Measurements section does not establish those advantages either. (LLM, conf=0.84)
Experimental — accuracy not yet measuredDLS has a narrow resolution, preventing recognition of particles differing from each other by a factor lower than 3 (e.g., 90 and 110 nm are not distinguished and a very high PDI is obtained), negatively impacting the correctness of data from polydisperse samples.
“In addition to the limitations described above, another weakness of DLS is its narrow resolution, preventing the possibility to recognize particles differing from each other for a factor lower than 3 (e.g.particles of 90 and 110 nm are not distinguished and a very high polydispersity index (PDI) is obtained) negatively impacting on the correctness of data obtained from polydisperse samples”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [133]
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Experimental — accuracy not yet measuredAFM has been indicated as a proper tool for micelles size determination due to its ability to distinguish particles with different dimensions in a mixture as well as to operate at room temperature, thus allowing the analysis of biological samples close to native and physiological conditions.
“AFM has been indicated as a proper tool for micelles size determination due to its ability to distinguish particles with different dimensions in a mixture as well as to operate at room temperature, thus allowing the analysis of biological samples close to native and physiological conditions [133,[154][155][156].”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [133]
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Experimental — accuracy not yet measuredDLS requires avoiding analysis of colored or fluorescent samples which could partially absorb the energy of the monochromatic light leading to an underestimation of particle size.
“Firstly, the need to avoid the analysis of colored or fluorescent samples which could partially absorb the energy of the monochromatic light leading to an underestimation of the particles size.”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [133]
The Introduction and Conclusions discuss DLS errors associated with particle shape and data-analysis software, not sample color or fluorescence. The Methods describe a laser-based measurement but do not say that light absorption by colored or fluorescent samples causes particle-size underestimation (searched: colored or fluorescent samples, absorption, laser, underestimation). (LLM, conf=0.74)
Experimental — accuracy not yet measured
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Cited by
Sorted by citations
Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., & Nicoli, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.031 · Cited by 1,135 · see citing works
- Authors
- Ree, B. J., Lee, J., Satoh, Y., Kwon, K., Isono, T., Satoh, T., & Ree, M.
- Affiliations
- Hokkaido University (JP)
- Department of Chemistry, Division of Advanced Materials Science, Polymer Research Institute, and Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, Korea
- Pohang University of Science and Technology (KR)
- Corresponding author
- Satoh, Toshifumi
- Ree, Moonhor
- Venue
- Polymers
- Publisher
- MDPI AG
- Published
- 2018-12-05
- Volume
- 10
- Issue
- 12
- Pages
- 1347
- ISSN
- 2073-4360 (ISSN-L)
- PMC
- PMC6401847
- OpenAlex
- W2903269357
- Cited by
- 31
- References
- 44
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 9 · 2020: 4 · 2019: 3
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Polymers (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology (18H04639)
- Japan Society for the Promotion of Science (16H04152)
- Topics
- Physical Sciences › Chemistry › Organic Chemistry › Surfactants and Colloidal Systems
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Physical Sciences › Materials Science › Materials Chemistry › Block Copolymer Self-Assembly
- Keywords
- polymeric micelles, micelle morphology, cylindrical micelles, micelle size distribution, synchrotron X-ray scattering, poly(ethylene glycol) block copolymers
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