CoChat
Sign Up
Cited by 50Bone Tissue Engineering Materialsbiodegradable polymer synthesis and propertiesAdditive Manufacturing and 3D Printing Technologies

FDM 3D Printed Composites for Bone Tissue Engineering Based on Plasticized Poly(3-hydroxybutyrate)/poly(d,l-lactide) Blends

Open access
Melčová, Veronika, Svoradová, Kateřina, Menčík, Přemysl, Kontárová, Soňa, Rampichová, Michala, Hedvičáková, Věra, Sovková, Věra, Přikryl, Radek +1 more·2020·Polymers
DOI 10.3390/polym12122806PMC PMC7761374
Open this paper in CoChat — free accountOpen PDF

Abstract

Tissue engineering is a current trend in the regenerative medicine putting pressure on scientists to develop highly functional materials and methods for scaffolds’ preparation. In this paper, the calibrated filaments for Fused Deposition Modeling (FDM) based on plasticized poly(3-hydroxybutyrate)/poly(d,l-lactide) 70/30 blend modified with tricalcium phosphate bioceramics were prepared. Two different plasticizers, Citroflex (n-Butyryl tri-n-hexyl citrate) and Syncroflex (oligomeric adipate ester), both used in the amount of 12 wt%, were compared. The printing parameters for these materials were optimized and the printability was evaluated by recently published warping test. The samples were studied with respect to their thermal and mechanical properties, followed by biological in vitro tests including proliferation, viability, and osteogenic differentiation of human mesenchymal stem cells. According to the results from differential scanning calorimetry and tensile measurements, the Citroflex-based plasticizer showed very good softening effect at the expense of worse printability and unsatisfactory performance during biological testing. On the other hand, the samples with Syncroflex demonstrated lower warping tendency compared to commercial polylactide filament with the warping coefficient one third lower. Moreover, the Syncroflex-based samples exhibited the non-cytotoxicity and promising biocompatibility.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3390/polym12122806

Cites

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • One advantage of combining PLA with bioceramics is the possibility of additive manufacturing, enabling a high degree of personalization.

    “One advantage of combining PLA with bioceramics is the possibility of additive manufacturing, enabling a high degree of personalization [169,188,189,190,191,192].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [192]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

No references could be resolved yet.

Research works printed but not linked: 34

  1. [1]Amin S. Achenbach S.J. Atkinson E.J. Khosla S. Melton L.J. Trends in Fracture Incidence: A Population-Based Study Over 20 Years J. Bone Miner. Res. 2014 29 581 589 10.1002/jbmr.2072 23959594 PMC3929546 · DOI
  2. [2]Johnell O. Kanis J.A. An estimate of the worldwide prevalence and disability associated with osteoporotic fractures Osteoporos. Int. 2006 17 1726 1733 10.1007/s00198-006-0172-4 16983459 · DOI
  3. [3]Brown J.L. Kumbar S.G. Laurencin C.T. Bone tissue engineering Biomaterials Science: An Introduction to Materials in Medicine 3rd ed. Elsevier Amsterdam, The Netherlands 2013 1194 1214
  4. [4]Laurencin C.T. Ambrosio A.M.A. Borden M.D. Cooper J.A. Tissue Engineering: Orthopedic Applications Annu. Rev. Biomed. Eng. 1999 1 19 46 10.1146/annurev.bioeng.1.1.19 11701481 · DOI
  5. [5]Fattahian H. Mansouri K. Mansouri N. Biomaterials, Substitutes, And Tissue Engineering in Bone Repair Comp. Clin. Path. 2019 28 879 891 10.1007/s00580-017-2507-2 · DOI
  6. [6]Qu H. Fu H. Han Z. Sun Y. Biomaterials for bone tissue engineering scaffolds: A review RSC Adv. 2019 9 26252 26262 10.1039/C9RA05214C PMC9070423 35531040 · DOI
  7. [7]Hollister S.J. Scaffold Design and Manufacturing: From Concept to Clinic Adv. Mater. 2009 21 3330 3342 10.1002/adma.200802977 20882500 · DOI
  8. [8]Chocholata P. Kulda V. Babuska V. Fabrication of Scaffolds for Bone-Tissue Regeneration Materials 2019 12 568 10.3390/ma12040568 PMC6416573 30769821 · DOI
  9. [9]Sloviková A. Vojtová L. Jančář J. Preparation and modification of collagen-based scaffold for tissue engineering Chem. Pap. 2008 62 417 422 0366-6352 10.2478/s11696-008-0045-8 · DOI
  10. [10]Švachová V. Vojtová L. Pavliňák D. Vojtek L. Sedláková V. Hyršl P. Alberti M. Jaroš J. Hampl A. Jančář J. Novel electrospun gelatin/oxycellulose nanofibers as a suitable platform for lung disease modeling Mater. Sci. Eng. C 2016 67 493 501 10.1016/j.msec.2016.05.059 27287147 · DOI
  11. [11]Balletti C. Ballarin M. Guerra F. 3D printing: State of the art and future perspectives J. Cult. Herit. 2017 26 172 182 10.1016/j.culher.2017.02.010 · DOI
  12. [12]Ngo T.D. Kashani A. Imbalzano G. Nguyen K.T. Hui D. Additive manufacturing (3D printing): A review of materials, methods, applications and challenges Compos. Part B Eng. 2018 143 172 196 10.1016/j.compositesb.2018.02.012 · DOI · Paper page
  13. [13]Pereira T.F. Oliveira M. Maia I.A. Da Silva J.V.L. Costa M. Thiré R.M.D.S.M. 3D Printing of Poly(3-Hydroxybutyrate) Porous Structures Using Selective Laser Sintering Macromol. Symp. 2012 319 64 73 10.1002/masy.201100237 · DOI
  14. [14]Lee S. Zhou W.Y. Wang M. Cheung W.L. Ip W.Y. Selective Laser Sintering of Poly(L-Lactide) Porous Scaffolds for Bone Tissue Engineering J. Biomim. Biomater. Tissue Eng. 2008 1 81 89 10.4028/www.scientific.net/JBBTE.1.81 · DOI
  15. [15]Saska S. Pires L.C. Cominotte M.A. Mendes L.S. de Oliveira M.F. Maia I.A. da Silva JV L. Ribeiro SJ L. Cirelli J.A. Three-Dimensional Printing and in Vitro Evaluation of Poly(3-Hydroxybutyrate) Scaffolds Functionalized with Osteogenic Growth Peptide for Tissue Engineering Mater. Sci. Eng. C 2018 89 265 273 10.1016/j.msec.2018.04.016 29752098 · DOI
  16. [16]Boparai K.S. Singh R. Singh H. Development of rapid tooling using fused deposition modeling: A review Rapid Prototyp. J. 2016 22 281 299 10.1108/RPJ-04-2014-0048 · DOI
  17. [17]Carrell J. Gruss G. Gomez E. Four-dimensional printing using fused-deposition modeling: A review Rapid Prototyp. J. 2020 26 855 869 10.1108/RPJ-12-2018-0305 · DOI
  18. [18]Dhinakaran V. Kumar K.M. Ram P.B. Ravichandran M. Vinayagamoorthy M. A review on recent advancements in fused deposition modeling Mater. Today Proc. 2020 27 752 756 10.1016/j.matpr.2019.12.036 · DOI
  19. [19]Ligon S.C. Liska R. Stampfl J. Gurr M. Mülhaupt R. Polymers for 3D Printing and Customized Additive Manufacturing Chem. Rev. 2017 117 10212 10290 10.1021/acs.chemrev.7b00074 28756658 PMC5553103 · DOI
  20. [20]Mohan N. Senthil P. Vinodh S. Jayanth N. A review on composite materials and process parameters optimisation for the fused deposition modelling process Virtual Phys. Prototyp. 2017 12 47 59 10.1080/17452759.2016.1274490 · DOI
  21. [21]Chiulan I. Frone A.N. Brandabur C. Panaitescu D.M. Recent Advances in 3D Printing of Aliphatic Polyesters Bioengineering 2018 5 2 10.3390/bioengineering5010002 PMC5874868 29295559 · DOI
  22. [22]Shunmugasundaram M. Maughal A.A.B. Ajay Kumar M. A review of bio-degradable materials for fused deposition modeling machine Mater. Today Proc. 2020 27 1596 1600 10.1016/j.matpr.2020.03.267 · DOI
  23. [23]Wurm M.C. Möst T. Bergauer B. Rietzel D. Neukam F.W. Cifuentes S.C. Von Wilmowsky C. In-vitro evaluation of Polylactic acid (PLA) manufactured by fused deposition modeling J. Biol. Eng. 2017 11 1 9 10.1186/s13036-017-0073-4 28919925 PMC5594599 · DOI
  24. [24]Lim J. You M. Li J. Li Z. Emerging bone tissue engineering via Polyhydroxyalkanoate (PHA)-based scaffolds Mater. Sci. Eng. C 2017 79 917 929 10.1016/j.msec.2017.05.132 28629097 · DOI
  25. [25]Butt F.I. Muhammad N. Hamid A. Moniruzzaman M. Sharif F. Recent progress in the utilization of biosynthesized polyhydroxyalkanoates for biomedical applications—Review Int. J. Biol. Macromol. 2018 120 1294 1305 10.1016/j.ijbiomac.2018.09.002 30189278 · DOI
  26. [26]Kovalcik A. Sangroniz L. Kalina M. Skopalova K. Humpolíček P. Omastova M. Mundigler N. Müller A.J. Properties of scaffolds prepared by fused deposition modeling of poly(hydroxyalkanoates) Int. J. Biol. Macromol. 2020 161 364 376 10.1016/j.ijbiomac.2020.06.022 32522546 · DOI
  27. [27]Gonzalez Ausejo J. Rydz J. Musioł M. Sikorska W. Sobota M. Włodarczyk J. Adamus G. Janeczek H. Kwiecień I. Hercog A. A comparative study of three-dimensional printing directions: The degradation and toxicological profile of a PLA/PHA blend Polym. Degrad. Stab. 2018 152 191 207 10.1016/j.polymdegradstab.2018.04.024 · DOI · Paper page
  28. [28]Esposito Corcione C. Scalera F. Gervaso F. Montagna F. Sannino A. Maffezzoli A. One-step solvent-free process for the fabrication of high loaded PLA/HA composite filament for 3D printing J. Therm. Anal. Calorim. 2018 134 575 582 10.1007/s10973-018-7155-5 · DOI
  29. [29]Xuan Y. Tang H. Wu B. Ding X. Lu Z. Li W. Xu Z. A specific groove design for individualized healing in a canine partial sternal defect model by a polycaprolactone/hydroxyapatite scaffold coated with bone marrow stromal cells J. Biomed. Mater. Res. Part A 2014 102 3401 3408 10.1002/jbm.a.35012 24142768 · DOI
  30. [30]Konopnicki S. Sharaf B. Resnick C. Patenaude A. Pogal-Sussman T. Hwang K.-G. Abukawa H. Troulis M.J. Tissue-Engineered Bone With 3-Dimensionally Printed β-Tricalcium Phosphate and Polycaprolactone Scaffolds and Early Implantation: An In Vivo Pilot Study in a Porcine Mandible Model J. Oral Maxillofac. Surg. 2015 73 1016.e1 1016.e11 10.1016/j.joms.2015.01.021 25883004 · DOI
  31. [31]Dávila J.L. Freitas M.S. Inforçatti Neto P. Silveira Z.C. Silva JV L. d’Ávila M.A. Fabrication of PCL/Β-TCP Scaffolds By 3D Mini-Screw Extrusion Printing J. Appl. Polym. Sci. 2016 133 133 10.1002/app.43031 · DOI
  32. [32]Samavedi S. Whittington A.R. Goldstein A.S. Calcium phosphate ceramics in bone tissue engineering: A review of properties and their influence on cell behavior Acta Biomater. 2013 9 8037 8045 10.1016/j.actbio.2013.06.014 23791671 · DOI
  33. [33]Kontárová S. Přikryl R. Melčová V. Menčík P. Horálek M. Figalla S. Plavec R. Feranc J. Sadílek J. Pospíšilová A. Printability, Mechanical and Thermal Properties of Poly(3-Hydroxybutyrate)-Poly(Lactic Acid)-Plasticizer Blends for Three-Dimensional (3D) Printing Materials 2020 13 4736 10.3390/ma13214736 PMC7660351 33114009 · DOI
  34. [34]Menčík P. Přikryl R. Stehnová I. Melčová V. Kontárová S. Figalla S. Alexy P. Bočkaj J. Effect of Selected Commercial Plasticizers on Mechanical, Thermal, and Morphological Properties of Poly(3-hydroxybutyrate)/Poly(lactic acid)/Plasticizer Biodegradable Blends for Three-Dimensional (3D) Print Materials 2018 11 1893 10.3390/ma11101893 PMC6213132 30282917 · DOI

Cited by

Sorted by citations

Authors
Melčová, V., Svoradová, K., Menčík, P., Kontárová, S., Rampichová, M., Hedvičáková, V., Sovková, V., Přikryl, R., & Vojtová, L.
Affiliations
  • Brno University of Technology (CZ)
  • Czech Academy of Sciences (CZ)
  • Central European Institute of Technology (CZ)
Corresponding author
  • Melčová, Veronika
  • Vojtová, Lucy
ORCID
Venue
Polymers
Publisher
MDPI AG
Published
2020-11-27
Volume
12
Issue
12
Pages
2806
ISSN
2073-4360 (ISSN-L)
DOI
10.3390/polym12122806
PMC
PMC7761374
OpenAlex
W3109322035
Cited by
50
References
0
Citations by year
2026: 5 · 2025: 10 · 2024: 16 · 2023: 10 · 2022: 9
FWCI
1.49
Type
journal article
Language
en
Open access
Licence
Locations
  • Polymers (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Brno University of Technology Digital Library (Brno University of Technology) (acceptedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Grantová Agentura České Republiky (18-09306S)
  • Ministerstvo Průmyslu a Obchodu (FV20480)
  • Vysoké Učení Technické v Brně (FCH-S-20-6340)
  • Ministerstvo Školství, Mládeže a Tělovýchovy (LQ1601)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Physical Sciences › Engineering › Automotive Engineering › Additive Manufacturing and 3D Printing Technologies
Keywords
fused deposition modeling, 3D-printed scaffolds, polyhydroxybutyrate, poly(D,L-lactide), polymer blends, plasticizers, tricalcium phosphate, printability, warpage, human mesenchymal stem cells, osteogenic differentiation, biocompatibility, thermal properties, mechanical properties

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review