The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays
Open accessAbstract
Accurate prediction of the adverse effects of test compounds on living systems, detection of toxic thresholds, and expansion of experimental data sets to include multiple toxicity end-point analysis are required for any robust screening regime. Alamar Blue is an important redox indicator that is used to evaluate metabolic function and cellular health. The Alamar Blue bioassay has been utilized over the past 50 years to assess cell viability and cytotoxicity in a range of biological and environmental systems and in a number of cell types including bacteria, yeast, fungi, protozoa and cultured mammalian and piscine cells. It offers several advantages over other metabolic indicators and other cytotoxicity assays. However, as with any bioassay, suitability must be determined for each application and cell model. This review seeks to highlight many of the important considerations involved in assay use and design in addition to the potential pitfalls.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
O'Brien, J., Wilson, I., Orton, T., & Pognan, F. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01606.x · Cited by 3,446
Franzblau, S. G., Witzig, R. S., McLaughlin, J. C., Torres, P., Madico, G., Hernandez, A., Degnan, M. T., Cook, M. B., Quenzer, V. K., Ferguson, R. M., et al. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.2.362-366.1998 · Cited by 964
Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis
Yajko, D. M., Madej, J. J., Lancaster, M. V., Sanders, C. A., Cawthon, V. L., Gee, B., Babst, A., & Hadley, W. K. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.9.2324-2327.1995 · Cited by 209
Erikstein, B. S., Hagland, H. R., Nikolaisen, J., Kulawiec, M., Singh, K. K., Gjertsen, B. T., & Tronstad, K. J. (2010) · Journal of Cellular Biochemistry · doi:10.1002/jcb.22741 · Cited by 54
Cited by
Wearable and Miniaturized Sensor Technologies for Personalized and Preventive Medicine
Tricoli, A., Nasiri, N., & De, S. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201605271 · Cited by 346
Nanayakkara, A. K., Follit, C. A., Chen, G., Williams, N. S., Vogel, P. D., & Wise, J. G. (2018) · Scientific Reports · doi:10.1038/s41598-018-19325-x · Cited by 282
Cell viability assessment using the Alamar blue assay: A comparison of 2D and 3D cell culture models
Bonnier, F., Keating, M. E., Wróbel, T. P., Majzner, K., Baranska, M., Garcia-Munoz, A., Blanco, A., & Byrne, H. J. (2015) · Toxicology in Vitro · doi:10.1016/j.tiv.2014.09.014 · Cited by 276
Leveraging Uncertainty in Machine Learning Accelerates Biological Discovery and Design
Hie, B., Bryson, B. D., & Berger, B. (2020) · Cell Systems · doi:10.1016/j.cels.2020.09.007 · Cited by 154
“Using the colorimetric, resazurin microtiter assay (alamar blue) (Lougheed et al., 2011; Rampersad, 2012), we determined the minimum inhibitory concentration (MIC) of these compounds as well as rifampicin, a frontline antibiotic for tuberculosis (Furin et al., 2019) (STAR Methods); the MICs for…”
MethodCosta, P., Gomes, A. T. P. C., Braz, M., Pereira, C., & Almeida, A. (2021) · Antibiotics · doi:10.3390/antibiotics10080974 · Cited by 77
Metadata
- Authors
- Rampersad, S. N.
- Venue
- Sensors
- Publisher
- MDPI AG
- Published
- 2012-09-10
- Volume
- 12
- Issue
- 9
- Pages
- 12347-12360
- ISSN
- 1424-8220 (ISSN-L)
- OpenAlex
- W2004602806
- Cited by
- 1,068
- References
- 4
- FWCI
- 31.55
- Type
- journal article
- Language
- en
- Open access
- gold
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays. https://app.cochat.ai/papers/10.3390/s120912347
- The paper
- Rampersad, S. N. (2012). Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Cellular Health in Cell Viability Bioassays. Sensors. https://doi.org/10.3390/s120912347
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
