The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis
Open accessAbstract
A colorimetric method for quantitative measurement of the susceptibility of Mycobacterium tuberculosis to antimicrobial agents is described. The method utilizes an oxidation-reduction dye, Alamar blue, as an indicator of growth. By this method, MICs of isoniazid, rifampin, streptomycin, and ethambutol were determined for 50 strains of M. tuberculosis. Colorimetric MIC results were available on the 7th, 10th, or 14th day of incubation for 29 (58%), 14 (28%), and 7 (14%) of the 50 strains, respectively. When MIC susceptibility results were compared with results obtained by the agar proportion method, increased levels of resistance detected by agar proportion were associated with higher MICs obtained by the colorimetric method. Tentative interpretive criteria for colorimetric MIC results which showed good agreement with results obtained by the agar proportion method were established. Interpretive agreement between the two methods was 98% for isoniazid, rifampin, and ethambutol and 94% for streptomycin. Overall, there was agreement between the two methods for 194 of 200 test results (97%). The colorimetric method is a rapid, quantitative, nonradiometric method for determining the antimicrobial susceptibility of M. tuberculosis.
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In the citing paper
“A rapid and inexpensive colorimetric method based on the oxidation-reduction indicators Alamar blue and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] have been successfully used for determining MICs of first-line drugs in DST of Mycobacterium tuberculosis (1, 9, 20, 31, 38, 49).”
From Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [49]
In the citing paper
“Preliminary studies of DST of MDR TB isolates using MTT, Alamar blue, or resazurin showed a good correlation with the conventional PM (1, 13, 19, 20, 31, 37, 49).”
From Resazurin Microtiter Assay Plate Testing of Mycobacterium tuberculosis Susceptibilities to Second-Line Drugs: Rapid, Simple, and Inexpensive Method (2003) · cites this paper as [49]
Citations
Cites
Public Health Mycobacteriology
Kent, P. (1985) · Medical Entomology and Zoology · Cited by 328
Lee, C.-N., & Heifets, L. B. (1987) · American Review of Respiratory Disease · doi:10.1164/ajrccm/136.2.349 · Cited by 91
Pfaller, M. A., Vũ, Q. D., Lancaster, M., Espinel‐Ingroff, A., Fothergill, A. W., Grant, C., McGinnis, M. R., Pasarell, L., Rinaldi, M. G., & Steele‐Moore, L. (1994) · Journal of Clinical Microbiology · doi:10.1128/jcm.32.7.1625-1628.1994 · Cited by 65
Self-citationA rapid method for determining the drug susceptibility of Mycobacterium tuberculosis.
Pital, A., Pital, R. C., & Leise, J. M. (1958) · PubMed · doi:10.1164/artpd.1958.78.1.111 · Cited by 7
Cited by
Gutierrez, M. G., Master, S. S., Singh, S. B., Taylor, G. A., Colombo, M. I., & Deretic, V. (2004) · Cell · doi:10.1016/j.cell.2004.11.038 · Cited by 2,252
Collins, L., & Franzblau, S. G. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.5.1004 · Cited by 1,599
Palomino, J.-C., Martin, A., Camacho, M., Guerra, H., Swings, J., & Portaels, F. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.8.2720-2722.2002 · Cited by 1,229
Rampersad, S. N. (2012) · Sensors · doi:10.3390/s120912347 · Cited by 1,068
Franzblau, S. G., Witzig, R. S., McLaughlin, J. C., Torres, P., Madico, G., Hernandez, A., Degnan, M. T., Cook, M. B., Quenzer, V. K., Ferguson, R. M., et al. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.2.362-366.1998 · Cited by 964
Metadata
- Authors
- Yajko, D. M., Madej, J. J., Lancaster, M. V., Sanders, C. A., Cawthon, V. L., Gee, B., Babst, A., & Hadley, W. K.
- Affiliations
- San Francisco General Hospital (US)
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1995-09
- Volume
- 33
- Issue
- 9
- Pages
- 2324-2327
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC228403
- OpenAlex
- W2133185708
- Cited by
- 209
- References
- 4
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 6 · 2019: 6 · 2018: 14 · 2017: 5 · 2016: 5 · 2015: 10 · 2014: 9 · 2013: 12 · 2012: 11
- FWCI
- 4.99
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- journals.asm.org/non-commercial-tdm-license (tdm)
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Keywords
- Mycobacterium tuberculosis, antimicrobial susceptibility, minimum inhibitory concentration, colorimetric assay, Alamar Blue, isoniazid, rifampicin, streptomycin, ethambutol, drug resistance, interpretation criteria
- MeSH terms
- Antibiotics, Antitubercular, Antitubercular Agents, Colorimetry, Ethambutol, Isoniazid, Microbial Sensitivity Tests, Mycobacterium tuberculosis, Rifampin, Streptomycin
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Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis. https://app.cochat.ai/papers/10.1128/jcm.33.9.2324-2327.1995
- The paper
- Yajko, D. M., Madej, J. J., Lancaster, M. V., Sanders, C. A., Cawthon, V. L., Gee, B., Babst, A., & Hadley, W. K. (1995). Colorimetric method for determining MICs of antimicrobial agents for Mycobacterium tuberculosis. Journal of Clinical Microbiology. https://doi.org/10.1128/jcm.33.9.2324-2327.1995
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
