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In Situ Real-Time Monitoring of Glutamate and Electrophysiology from Cortex to Hippocampus in Mice Based on a Microelectrode Array
Open accessAbstract
Changes in the structure and function of the hippocampus contribute to epilepsy, schizophrenia and other neurological or mental disorders of the brain. Since the function of the hippocampus depends heavily on the glutamate (Glu) signaling pathways, in situ real-time detection of Glu neurotransmitter release and electrophysiological signals in hippocampus is of great significance. To achieve the dual-mode detection in mouse hippocampus in vivo, a 16-channel implantable microelectrode array (MEA) was fabricated by micro-electromechanical system (MEMS) technology. Twelve microelectrode sites were modified with platinum black for electrophysiological recording and four sites were modified with glutamate oxidase (GluOx) and 1,3-phenylenediamine (mPD) for selective electrochemical detection of Glu. The MEA was implanted from cortex to hippocampus in mouse brain for in situ real-time monitoring of Glu and electrophysiological signals. It was found that the Glu concentration in hippocampus was roughly 50 μM higher than that in the cortex, and the firing rate of concurrently recorded spikes declined from 6.32 ± 4.35 spikes/s in cortex to 0.09 ± 0.06 spikes/s in hippocampus. The present results demonstrated that the dual-mode MEA probe was capable in neurological detections in vivo with high spatial resolution and dynamical response, which lays the foundation for further pathology studies in the hippocampus of mouse models with nervous or mental disorders.
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miRNA-mediated disturbance of neurotransmitters including glutamate, dopamine, serotonin, and norepinephrine can lead to neuronal damage and loss of trophic effects and thereby potentially contribute to the pathogenesis of depression.
“miRNA-mediated disturbance of neurotransmitters, including glutamate, dopamine, serotonin and norepinephrine, can lead to neuronal damage, and loss of trophic effects and thereby potentially contribute to the pathogenesis of depression.271–274”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [271]
The Introduction identifies glutamate as a neurotransmitter important to hippocampal function and broadly links glutamate signaling to nervous or mental disease. It does not address miRNAs, dopamine, serotonin, norepinephrine, neuronal damage, loss of trophic effects, or depression specifically. The indexed passages provide section labels but no page numbers. (LLM, conf=0.86)
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Metadata
- Authors
- Fan, X., Song, Y., Ma, Y., Zhang, S., Xiao, G., Yang, L., Xu, H., Zhang, D., & Cai, X.
- Affiliations
- Chinese Academy of Sciences (CN)
- Peking University (CN)
- Corresponding author
- Cai, Xinxia
- Venue
- Sensors
- Publisher
- MDPI AG
- Published
- 2016-12-30
- Volume
- 17
- Issue
- 1
- Pages
- 61
- ISSN
- 1424-8220 (ISSN-L)
- PMC
- PMC5298634
- OpenAlex
- W2566699448
- Cited by
- 30
- References
- 20
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 5 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 7 · 2018: 4 · 2017: 1
- FWCI
- 2.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Sensors (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- the National Natural Science Foundation (61527815, 61501426, 31500800, 61601435)
- the Key Programs of the Chinese Academy of Sciences (KJZD-EW-L11–2)
- the Beijing Science and Technology Plan (Z141100000214002)
- Open Project of Beijing Key Laboratory on Research of Parkinson's Disease
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Keywords
- glutamate detection, hippocampus, microelectrode arrays, implantable neural probes, glutamate oxidase, m-phenylenediamine, platinum black electrodes, electrophysiological recording, mouse brain, epilepsy, schizophrenia
- MeSH terms
- Animals, Electrophysiology, Hippocampus, Microelectrodes, Phenylenediamines, Biosensing Techniques, Glutamic Acid, Mice, Micro-Electrical-Mechanical Systems
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