Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives
Open accessAbstract
Continuous monitoring and early warning of potential water contamination with toxic chemicals is of paramount importance for human health and sustainable food production. During the last few decades there have been noteworthy advances in technologies for the automated sensing of physicochemical parameters of water. These do not translate well into online monitoring of chemical pollutants since most of them are either incapable of real-time detection or unable to detect impacts on biological organisms. As a result, biological early warning systems have been proposed to supplement conventional water quality test strategies. Such systems can continuously evaluate physiological parameters of suitable aquatic species and alert the user to the presence of toxicants. In this regard, single cellular organisms, such as bacteria, cyanobacteria, micro-algae and vertebrate cell lines, offer promising avenues for development of water biosensors. Historically, only a handful of systems utilising single-cell organisms have been deployed as established online water biomonitoring tools. Recent advances in recombinant microorganisms, cell immobilisation techniques, live-cell microarrays and microfluidic Lab-on-a-Chip technologies open new avenues to develop miniaturised systems capable of detecting a broad range of water contaminants. In experimental settings, they have been shown as sensitive and rapid biosensors with capabilities to detect traces of contaminants. In this work, we critically review the recent advances and practical prospects of biological early warning systems based on live-cell biosensors. We demonstrate historical deployment successes, technological innovations, as well as current challenges for the broader deployment of live-cell biosensors in the monitoring of water quality.
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Flow cytometry can be used as a rapid and reliable off-line method for monitoring lipid content.
“Flow cytometry can be used as a rapid and reliable off-line method for monitoring lipid content [233].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [233]
Section 3 describes flow cytometry as a gold standard for cell analysis and reports rapid algal cytotoxicity assays. It does not establish that flow cytometry is a reliable off-line method for monitoring lipid content; its monitoring examples concern bacterial viability, cell counts and water quality. Section 6.4 likewise discusses chlorophyll fluorescence and cell-health markers, not lipids. No page numbers were provided in our copy. (LLM, conf=0.79)
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Cites
Creating new fluorescent probes for cell biology
Zhang, J., Campbell, R. E., Ting, A. Y., & Tsien, R. Y. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm976 · Cited by 1,984 · see citing works
Ronkainen, N. J., Halsall, H. B., & Heineman, W. R. (2010) · Chemical Society Reviews · doi:10.1039/b714449k · Cited by 1,975 · see citing works
Waterborne transmission of protozoan parasites: Review of worldwide outbreaks - An update 2011–2016
Efstratiou, A., Ongerth, J. E., & Karanis, P. (2017) · Water Research · doi:10.1016/j.watres.2017.01.036 · Cited by 1,109 · see citing works
A morphological biosensor for mammalian cells
Giæver, I., & Keese, C. R. (1993) · Nature · doi:10.1038/366591a0 · Cited by 775 · see citing works
Wegener, J., Keese, C. R., & Giæver, I. (2000) · Experimental Cell Research · doi:10.1006/excr.2000.4919 · Cited by 773 · see citing works
Molecular biology of bacterial bioluminescence
Meighen, E. A. (1991) · Microbiological Reviews · doi:10.1128/mr.55.1.123-142.1991 · Cited by 672 · see citing works
Microbial whole-cell sensing systems of environmental pollutants
Belkin, S. (2003) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(03)00059-6 · Cited by 523 · see citing works
The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability
Ning, K., Wang, X., Xu, X., & Abassi, Y. A. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-108-6_6 · Cited by 414 · see citing works
Advances in on-line drinking water quality monitoring and early warning systems
Storey, M. V., van der Gaag, B., & Burns, B. P. (2010) · Water Research · doi:10.1016/j.watres.2010.08.049 · Cited by 383 · see citing works
Bioluminescent sensors for detection of bioavailable Hg(II) in the environment
Selifonova, O., Burlage, R. S., & Barkay, T. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.9.3083-3090.1993 · Cited by 359 · see citing works
Stocker, J., Balluch, D., Gsell, M., Harms, H., Feliciano, J. S., Daunert, S., Malik, K. A., & van der Meer, J. R. (2003) · Environmental Science & Technology · doi:10.1021/es034258b · Cited by 335 · see citing works
Impedance-Based Detection of Bacteria
Furst, A. L., & Francis, M. B. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00381 · Cited by 329 · see citing works
Prest, E. I., Hammes, F., Kötzsch, S., van Loosdrecht, M. C. M., & Vrouwenvelder, J. S. (2013) · Water Research · doi:10.1016/j.watres.2013.07.051 · Cited by 308 · see citing works
Rapid and sensitive pollutant detection by induction of heat shock gene-bioluminescence gene fusions
Dyk, T. K. V., Majarian, W. R., Konstantinov, K. B., Young, R. M., Dhurjati, P., & LaRossa, R. A. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.5.1414-1420.1994 · Cited by 299 · see citing works
Phenotyping Plant Responses to Biotic Stress by Chlorophyll Fluorescence Imaging
Pérez‐Bueno, M. L., Pineda, M., & Barón, M. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01135 · Cited by 287 · see citing works
Oxborough, K. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/erh145 · Cited by 262 · see citing works
Heitzer, A., Malachowsky, K., Thonnard, J. E., Bienkowski, P. R., White, D. C., & Sayler, G. S. (1994) · Applied and Environmental Microbiology · doi:10.1128/aem.60.5.1487-1494.1994 · Cited by 248 · see citing works
Bae, M., & Park, Y. (2013) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2013.07.075 · Cited by 246 · see citing works
Eubanks, L. M., Dickerson, T. J., & Janda, K. D. (2007) · Chemical Society Reviews · doi:10.1039/b615227a · Cited by 218 · see citing works
Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill
Schirmer, K., Dixon, D. G., Greenberg, B. M., & Bols, N. C. (1998) · Toxicology · doi:10.1016/s0300-483x(98)00030-4 · Cited by 215 · see citing works
A novel biomonitoring system using microbial fuel cells
Kim, M., Hyun, M. S., Gadd, G. M., & Kim, H. J. (2007) · Journal of Environmental Monitoring · doi:10.1039/b713114c · Cited by 213 · see citing works
Chouteau, C., Dzyadevych, S. V., Durrieu, C., & Chovelon, J. (2004) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.09.032 · Cited by 211 · see citing works
Biomonitors and Biomarkers as Indicators of Environmental Change 2
Butterworth, F. M., Gunatilaka, A., & Gonsebatt, M. E. (2001) · Springer eBooks · doi:10.1007/978-1-4615-1305-6 · Cited by 209 · see citing works
Impedance microbiology—a rapid change for microbiologists
Silley, P., & Forsythe, S. J. (1996) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1996.tb03215.x · Cited by 204 · see citing works
Ivask, A., Rõlova, T., & Kahru, A. (2009) · BMC Biotechnology · doi:10.1186/1472-6750-9-41 · Cited by 204 · see citing works
Cohen, E., Levy, G. J., & Borisover, M. D. (2014) · Water Research · doi:10.1016/j.watres.2014.02.040 · Cited by 203 · see citing works
Franklin, N. M., Stauber, J. L., & Lim, R. P. (2001) · Environmental Toxicology and Chemistry · doi:10.1002/etc.5620200118 · Cited by 193 · see citing works
Whole-Cell-Based Biosensors for Environmental Biomonitoring and Application
Gu, M. B., Mitchell, R. J., & Kim, B. C. (2004) · Advances in biochemical engineering, biotechnology · doi:10.1007/b13533 · Cited by 187 · see citing works
Optical whole-cell biosensor using Chlorella vulgaris designed for monitoring herbicides
Védrine, C., Leclerc, J., Durrieu, C., & Tran‐Minh, C. (2003) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(02)00157-4 · Cited by 178 · see citing works
An algal biosensor for the monitoring of water toxicity in estuarine environments
Campanella, L., Cubadda, F., Sammartino, M. P., & Saoncella, A. (2001) · Water Research · doi:10.1016/s0043-1354(00)00223-2 · Cited by 173 · see citing works
Machdar, E., Steen, P., Raschid-Sally, L., & Lens, P. N. L. (2013) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2013.01.048 · Cited by 172 · see citing works
Technology Challenges in Responding to Biological or Chemical Attacks in the Civilian Sector
Fitch, J. P., Raber, E., & Imbro, D. R. (2003) · Science · doi:10.1126/science.1085922 · Cited by 170 · see citing works
Microbial Fuel Cell-Based Biosensors
Cui, Y., Lai, B., & Tang, X. (2019) · Biosensors · doi:10.3390/bios9030092 · Cited by 169 · see citing works
Recent developments in potentiometric biosensors for biomedical analysis
Koncki, R. (2007) · Analytica Chimica Acta · doi:10.1016/j.aca.2007.08.003 · Cited by 157 · see citing works
A batch-mode cube microbial fuel cell based “shock” biosensor for wastewater quality monitoring
Liu, B., Lei, Y., & Li, B. (2014) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2014.06.051 · Cited by 153 · see citing works
Comparison of three microbial toxicity screening tests with the microtox test
Dutka, B. J., & Kwan, K. K. (1981) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/bf01611091 · Cited by 148 · see citing works
Schreiber, U., Quayle, P., Schmidt, S., Escher, B. I., & Mueller, J. F. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2006.10.018 · Cited by 146 · see citing works
Miao, A., Wang, W., & Juneau, P. (2005) · Environmental Toxicology and Chemistry · doi:10.1897/05-009r.1 · Cited by 143 · see citing works
Using higher organisms in biological early warning systems for real-time toxicity detection
van der Schalie, W. H., Shedd, T. R., Knechtges, P. L., & Widder, M. W. (2001) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(01)00160-9 · Cited by 140 · see citing works
Stiner, L., & Halverson, L. J. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1962-1971.2002 · Cited by 140 · see citing works
A cell array biosensor for environmental toxicity analysis
Lee, J. H., Mitchell, R. J., Kim, B. C., Cullen, D. C., & Gu, M. B. (2005) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.12.015 · Cited by 137 · see citing works
Hakkila, K., Green, T., Leskinen, P., Ivask, A., Marks, R. S., & Virta, M. (2004) · Journal of Applied Toxicology · doi:10.1002/jat.1020 · Cited by 136 · see citing works
Mauriz, E., Calle, A., Abad‐Fuentes, A., Montoya, A., Hildebrandt, A., Barceló, ., & Lechuga, L. M. (2005) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2005.10.013 · Cited by 134 · see citing works
The dynamic microbe: green fluorescent protein brings bacteria to light
Southward, C. M., & Surette, M. G. (2002) · Molecular Microbiology · doi:10.1046/j.1365-2958.2002.03089.x · Cited by 133 · see citing works
Felice, C. J., Madrid, R. E., Olivera, J. M., Rotger, V. I., & Valentinuzzi, M. E. (1999) · Journal of Microbiological Methods · doi:10.1016/s0167-7012(98)00098-0 · Cited by 128 · see citing works
Microbial Fuels Cell-Based Biosensor for Toxicity Detection: A Review
Zhou, T., Han, H., Liu, P., Xiong, J., Tian, F., & Li, X. (2017) · Sensors · doi:10.3390/s17102230 · Cited by 128 · see citing works
New type of dual-channel PAM chlorophyll fluorometer for highly sensitive water toxicity biotests
Schreiber, U., Mueller, J. F., Haugg, A. M., & Gademann, R. (2002) · Photosynthesis Research · doi:10.1023/a:1021276003145 · Cited by 127 · see citing works
Nabok, A. V., Tsargorodskaya, A., Hassan, A. K., & Starodub, N. F. (2005) · Applied Surface Science · doi:10.1016/j.apsusc.2004.11.084 · Cited by 125 · see citing works
Algal fluorescence sensor integrated into a microfluidic chip for water pollutant detection
Lefèvre, F., Chalifour, A., Yu, L., Chodavarapu, V. P., Juneau, P., & Izquierdo, R. (2011) · Lab on a Chip · doi:10.1039/c2lc20998e · Cited by 124 · see citing works
Potential use of flow cytometry in toxicity studies with microalgae
Franqueira, D., García, M. O., Torres, E., Herrero, C., & Cid, Á. (2000) · The Science of The Total Environment · doi:10.1016/s0048-9697(99)00483-0 · Cited by 121 · see citing works
Optical imaging fiber-based live bacterial cell array biosensor
Biran, I., Rissin, D. M., Ron, E. Z., & Walt, D. R. (2003) · Analytical Biochemistry · doi:10.1016/s0003-2697(02)00700-5 · Cited by 120 · see citing works
Applications and potential uses of fish gill cell lines: examples with RTgill-W1
Lee, L. E. J., Dayeh, V. R., Schirmer, K., & Bols, N. C. (2009) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-008-9173-2 · Cited by 119 · see citing works
Woutersen, M., Belkin, S., Brouwer, B. A., van Wezel, A. P., & Heringa, M. B. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4372-6 · Cited by 118 · see citing works
Sorensen, J., Lapworth, D. J., Marchant, B. P., Nkhuwa, D. C. W., Pedley, S., Stuart, M. E., Bell, R. A., Chirwa, M., Kabika, J., Liemisa, M., et al. (2015) · Water Research · doi:10.1016/j.watres.2015.05.035 · Cited by 114 · see citing works
Eltzov, E., & Marks, R. S. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4084-y · Cited by 114 · see citing works
Portable LED fluorescence instrumentation for the rapid assessment of potable water quality
Bridgeman, J. O., Baker, A., Brown, D., & Boxall, J. B. (2015) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2015.04.050 · Cited by 113 · see citing works
Online and in situ monitoring of environmental pollutants: electrochemical biosensing of cadmium
Biran, I., Babai, R., Klimentiy, L., Rishpon, J., & Ron, E. Z. (2000) · Environmental Microbiology · doi:10.1046/j.1462-2920.2000.00103.x · Cited by 113 · see citing works
Bechor, O., Smulski, D. R., Dyk, T. K. V., LaRossa, R. A., & Belkin, S. (2002) · Journal of Biotechnology · doi:10.1016/s0168-1656(01)00423-0 · Cited by 113 · see citing works
Sorensen, J., Vivanco, A., Ascott, M., Gooddy, D. C., Lapworth, D. J., Read, D. S., Rushworth, C. M., Bucknall, J., Herbert, K., Karapanos, I., et al. (2018) · Water Research · doi:10.1016/j.watres.2018.03.001 · Cited by 112 · see citing works
ElMekawy, A., Hegab, H. M., Pant, D., & Saint, C. P. (2017) · Journal of Applied Microbiology · doi:10.1111/jam.13631 · Cited by 111 · see citing works
A portable cell-based impedance sensor for toxicity testing of drinking water
Curtis, T. M., Widder, M. W., Brennan, L. M., Schwager, S. J., van der Schalie, W. H., Fey, J. P., & Salazar, N. (2009) · Lab on a Chip · doi:10.1039/b901314h · Cited by 107 · see citing works
Impedance Microbiology: Applications in Food Hygiene
Wawerla, M., Stolle, A., Schalch, B., & Eisgruber, H. G. (1999) · Journal of Food Protection · doi:10.4315/0362-028x-62.12.1488 · Cited by 106 · see citing works
Applications of flow cytometry to ecotoxicity testing using microalgae
Stauber, J. L., Franklin, N. M., & Adams, M. S. (2002) · Trends in biotechnology · doi:10.1016/s0167-7799(01)01924-2 · Cited by 106 · see citing works
Brayner, R., Couté, A., Livage, J., Perrette, C., & Sicard, C. (2011) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-011-5107-z · Cited by 106 · see citing works
Fiber Optic Biosensor Using Chlorella vulgaris for Determination of Toxic Compounds
Naessens, M., Leclerc, J. C., & Tran‐Minh, C. (2000) · Ecotoxicology and Environmental Safety · doi:10.1006/eesa.1999.1904 · Cited by 105 · see citing works
Construction of a Bioluminescent Reporter Strain To Detect Polychlorinated Biphenyls
Layton, A., Muccini, M., Ghosh, M. M., & Sayler, G. S. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.12.5023-5026.1998 · Cited by 99 · see citing works
Use of the microtox® assay system for environmental samples
Chang, J. C., Taylor, P. B., & Leach, F. R. (1981) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/bf01622069 · Cited by 96 · see citing works
Muller, R., Schreiber, U., Escher, B. I., Quayle, P., Nash, S. B., & Mueller, J. F. (2008) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2008.02.062 · Cited by 95 · see citing works
Novel Cyanobacterial Biosensor for Detection of Herbicides
Shao, C. Y., Howe, C. J., Porter, A. J., & Glover, L. A. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.10.5026-5033.2002 · Cited by 92 · see citing works
Tsopela, A., Laborde, A., Salvagnac, L., Ventalon, V., Bedel‐Pereira, E., Séguy, I., Temple‐Boyer, P., Juneau, P., Izquierdo, R., & Launay, J. (2015) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2015.12.050 · Cited by 86 · see citing works
Bacteria Detection and Differentiation Using Impedance Flow Cytometry
Clausen, C. H., Dimaki, M., Bertelsen, C. V., Skands, G. E., Rodriguez-Trujíllo, R., Thomsen, J. D., & Svendsen, W. E. (2018) · Sensors · doi:10.3390/s18103496 · Cited by 85 · see citing works
Stewart, G. N. (1899) · The Journal of Experimental Medicine · doi:10.1084/jem.4.2.235 · Cited by 83 · see citing works
Analysis of bioavailable phenols from natural samples by recombinant luminescent bacterial sensors
Leedjärv, A., Ivask, A., Virta, M., & Kahru, A. (2006) · Chemosphere · doi:10.1016/j.chemosphere.2006.01.026 · Cited by 78 · see citing works
Besmer, M. D., Epting, J., Page, R. M., Sigrist, J. A., Huggenberger, P., & Hammes, F. (2016) · Scientific Reports · doi:10.1038/srep38462 · Cited by 78 · see citing works
Adekunle, A., Raghavan, V., & Tartakovsky, B. (2018) · Bioelectrochemistry · doi:10.1016/j.bioelechem.2018.11.007 · Cited by 77 · see citing works
Gill cell culture systems as models for aquatic environmental monitoring
Bury, N. R., Schnell, S., & Högstrand, C. (2014) · Journal of Experimental Biology · doi:10.1242/jeb.095430 · Cited by 69 · see citing works
Bacterial genotoxicity bioreporters
Biran, A., Yagur‐Kroll, S., Pedahzur, R., Buchinger, S., Reifferscheid, G., Ben‐Yoav, H., Shacham‐Diamand, Y., & Belkin, S. (2009) · Microbial Biotechnology · doi:10.1111/j.1751-7915.2009.00160.x · Cited by 65 · see citing works
An integrated mini biosensor system for continuous water toxicity monitoring
Lee, J. H., & Gu, M. B. (2004) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.06.036 · Cited by 65 · see citing works
Monitoring of surface water by ultrasensitiveDaphnia toximeter
Lechelt, M., Blohm, W., Kirschneit, B., Pfeiffer, M., Gresens, E., Liley, J., Pol-Holz, R. H. D., L�ring, C., & Moldaenke, C. (2000) · Environmental Toxicology · doi:10.1002/1522-7278(2000)15:5 · Cited by 64 · see citing works
Microtox® andSpirillum volutans tests for assessing toxicity of environmental samples
Coleman, R. N., & Qureshi, A. A. (1985) · Bulletin of Environmental Contamination and Toxicology · doi:10.1007/bf01636536 · Cited by 64 · see citing works
In situ on-line toxicity biomonitoring in water: Recent developments
Gerhardt, A., Ingram, M. K., Kang, I. J., & Ulitzur, S. (2006) · Environmental Toxicology and Chemistry · doi:10.1897/05-486r1.1 · Cited by 63 · see citing works
Wong, L. S., Lee, Y. H., & Surif, S. (2013) · Sensors · doi:10.3390/s130506394 · Cited by 62 · see citing works
The selection of a model microalgal species as biomaterial for a novel aquatic phytotoxicity assay
Nash, S. B., Quayle, P., Schreiber, U., & Mueller, J. F. (2005) · Aquatic Toxicology · doi:10.1016/j.aquatox.2005.02.004 · Cited by 61 · see citing works
Besmer, M. D., & Hammes, F. (2016) · Water Research · doi:10.1016/j.watres.2016.10.041 · Cited by 61 · see citing works
Yan, G., Du, Q., Wei, X., Miozzi, J., Chen, K., Wang, J., Han, X., Pan, J., Xie, H., Chen, J., et al. (2018) · Molecules · doi:10.3390/molecules23123280 · Cited by 61 · see citing works
Fabrication of a bio-MEMS based cell-chip for toxicity monitoring
Yoo, S. K., Lee, J. H., Yun, S.-S., Gu, M. B., & Lee, J. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2006.07.014 · Cited by 60 · see citing works
Schreiter, P., Gillor, O., Post, A. F., Belkin, S., Schmid, R. D., & Bachmann, T. T. (2001) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(01)00224-x · Cited by 59 · see citing works
Reevaluating multicolor flow cytometry to assess microbial viability
Buysschaert, B., Byloos, B., Leys, N., Houdt, R. V., & Boon, N. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7837-5 · Cited by 58 · see citing works
Lei, Y., Mulchandani, P., Chen, W., & Mulchandani, A. (2007) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-007-9023-9 · Cited by 58 · see citing works
Selection of a battery of rapid toxicity sensors for drinking water evaluation
van der Schalie, W. H., James, R. R., & Gargan, T. P. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2005.11.019 · Cited by 57 · see citing works
An oxidative stress-specific bacterial cell array chip for toxicity analysis
Lee, J. H., Youn, C. H., Kim, B. C., & Gu, M. B. (2006) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2006.10.038 · Cited by 56 · see citing works
Medium and interface components in impedance microbiology
Felice, C. J., & Valentinuzzi, M. E. (1999) · IEEE Transactions on Biomedical Engineering · doi:10.1109/10.804577 · Cited by 56 · see citing works
Skládal, P., Morozova, N. O., & Reshetilov, A. N. (2002) · Biosensors and Bioelectronics · doi:10.1016/s0956-5663(02)00076-3 · Cited by 54 · see citing works
Kawazumi, H., Gobi, K. V., Ogino, K., Maeda, H., & Miura, N. (2005) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2004.11.069 · Cited by 53 · see citing works
Detection of chemical threat agents in drinking water by an early warning real‐time biomonitor
Green, U., Kremer, J. H., Zillmer, M., & Moldaenke, C. (2003) · Environmental Toxicology · doi:10.1002/tox.10138 · Cited by 52 · see citing works
An advanced investigation on a new algal sensor determining Pb(II) ions from aqueous media
Yüce, M., Nazır, H., & Dönmez, G. (2010) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2010.08.022 · Cited by 52 · see citing works
Belaïdi, F. S., Farouil, L., Salvagnac, L., Temple‐Boyer, P., Séguy, I., Heully, J., Alary, F., Bedel‐Pereira, E., & Launay, J. (2019) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2019.01.065 · Cited by 52 · see citing works
Hong, S., Choi, S.-J., Jeong, H. D., & Hong, S. (2008) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2008.08.027 · Cited by 51 · see citing works
Hassannejad, S., Lotfi, R., Ghafarbi, S. P., Oukarroum, A., Abbasi, A., Kalaji, H. M., & Rastogi, A. (2020) · Plants · doi:10.3390/plants9040529 · Cited by 51 · see citing works
Advances in real-time monitoring of water quality using automated analysis of animal behaviour
Bownik, A., & Włodkowic, D. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.147796 · Cited by 49 · see citing works
Detection of Escherichia coli in potable water using direct impedance technology
Colquhoun, K. O., Timms, S., & Fricker, C. R. (1995) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1995.tb00948.x · Cited by 48 · see citing works
Mancini, J. A., Boylan, M. O., Soly, R. R., Graham, A., & Meighen, E. A. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68222-5 · Cited by 47 · see citing works
Hnaien, M., Lagarde, F., Bausells, J., Errachid, A., & Jaffrezic-Renault, N. J. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4336-x · Cited by 47 · see citing works
Improved cell sensitivity and longevity in a rapid impedance‐based toxicity sensor
Curtis, T. M., Tabb, J. S., Romeo, L. E., Schwager, S. J., Widder, M. W., & van der Schalie, W. H. (2009) · Journal of Applied Toxicology · doi:10.1002/jat.1421 · Cited by 47 · see citing works
Bioavailable nitrate detection in water by an immobilized luminescent cyanobacterial reporter strain
Mbeunkui, F., RICHAUD, C., Etienne, A., Schmid, R. D., & Bachmann, T. T. (2002) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-002-1139-9 · Cited by 46 · see citing works
Charrier, T., Durand, M.-J., Jouanneau, S., Dion, M., Pernetti, M., Poncelet, D., & Thouand, G. (2010) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-010-4353-9 · Cited by 46 · see citing works
Internal Response Correction for Fluorescent Whole-Cell Biosensors
Mirasoli, M., Feliciano, J. S., Michelini, E., Daunert, S., & Roda, A. (2002) · Analytical Chemistry · doi:10.1021/ac0259008 · Cited by 45 · see citing works
Gäberlein, S., Spener, F., & Zaborosch, C. (2000) · Applied Microbiology and Biotechnology · doi:10.1007/s002530000437 · Cited by 45 · see citing works
Ecotoxicology Goes on a Chip: Embracing Miniaturized Bioanalysis in Aquatic Risk Assessment
Campana, O., & Włodkowic, D. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b03370 · Cited by 45 · see citing works
Brennan, L. M., Widder, M. W., Lee, L. E. J., & van der Schalie, W. H. (2012) · Toxicology in Vitro · doi:10.1016/j.tiv.2012.03.010 · Cited by 44 · see citing works
Thouand, G., Horry, H., Durand, M.-J., Picart, P., Bendriaa, L., Daniel, P., & DuBow, M. S. (2003) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1279-6 · Cited by 42 · see citing works
Niazi, J. H., Kim, B. C., Ahn, J., & Gu, M. B. (2008) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2008.06.026 · Cited by 39 · see citing works
Kumar, S., Kundu, S., Pakshirajan, K., & Dasu, V. V. (2008) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-008-8280-6 · Cited by 38 · see citing works
Application of Bacterial Bioluminescence To Assess the Efficacy of Fast-Acting Biocides
Robinson, G. M., Tonks, K. M., Thorn, R. M. S., & Reynolds, D. M. (2011) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00489-11 · Cited by 38 · see citing works
Disposable potentiometric enzyme sensor for direct determination of organophosphorus insecticides
Gäberlein, S., Knoll, M., Zaborosch, C., & Spener, F. (2000) · The Analyst · doi:10.1039/b006664h · Cited by 37 · see citing works
CMOS Conductometric System for Growth Monitoring and Sensing of Bacteria
Yao, L., Lamarche, P., Tawil, N., Khan, R., Aliakbar, A., Hajj-Hassan, M., Chodavarapu, V. P., & Mandeville, R. (2010) · IEEE Transactions on Biomedical Circuits and Systems · doi:10.1109/tbcas.2010.2089794 · Cited by 36 · see citing works
Immobilization of planktonic algal spores by inkjet printing
Lee, H., Jung, S. M., Yoon, S., Park, T. H., Park, T. H., Kim, S., Shin, H. W., Hwang, D. S., & Jung, S. (2019) · Scientific Reports · doi:10.1038/s41598-019-48776-z · Cited by 36 · see citing works
A primary FIsh Gill Cell System (FIGCS) for environmental monitoring of river waters
Minghetti, M., Schnell, S., Chadwick, M. A., Högstrand, C., & Bury, N. R. (2014) · Aquatic Toxicology · doi:10.1016/j.aquatox.2014.05.019 · Cited by 33 · see citing works
Multiparameter Lab-on-a-Chip flow cytometry of the cellcycle
Skommer, J., Akagi, J., Takeda, K., Fujimura, Y., Khoshmanesh, K., & Włodkowic, D. (2012) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2012.11.008 · Cited by 33 · see citing works
Kubisch, R., Bohrn, U., Fleischer, M., & Stütz, E. (2012) · Sensors · doi:10.3390/s120303370 · Cited by 31 · see citing works
Shitanda, I., Takada, K., Sakai, Y., & Tatsuma, T. (2005) · Analytical Chemistry · doi:10.1021/ac050894b · Cited by 31 · see citing works
Rise of the Micromachines: Microfluidics and the Future of Cytometry
Włodkowic, D., & Darżynkiewicz, Z. (2011) · Methods in cell biology · doi:10.1016/b978-0-12-374912-3.00005-5 · Cited by 30 · see citing works
Bohrn, U., Mucha, A., Werner, C. F., Trattner, B., Bäcker, M., Krumbe, C., Schienle, M., Stütz, E., Schmitt‐Landsiedel, D., Fleischer, M., et al. (2013) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2013.02.105 · Cited by 30 · see citing works
Development of a broad-spectrum fluorescent heavy metal bacterial biosensor
Gireesh-Babu, P., & Chaudhari, A. (2012) · Molecular Biology Reports · doi:10.1007/s11033-012-2033-x · Cited by 29 · see citing works
Farré, M., & Barceló, . (2001) · Fresenius Journal of Analytical Chemistry · doi:10.1007/s002160100925 · Cited by 29 · see citing works
Hupp, S., Rosenkranz, M., Bonfig, K. B., Pandey, C., & Roitsch, T. G. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.01239 · Cited by 26 · see citing works
Transforming cyanobacteria into bioreporters of biological relevance
Bachmann, T. T. (2003) · Trends in biotechnology · doi:10.1016/s0167-7799(03)00114-8 · Cited by 25 · see citing works
Rakers, S., Imse, F., & Gebert, M. (2014) · Ecotoxicology · doi:10.1007/s10646-014-1279-6 · Cited by 25 · see citing works
Okafor, C. C., Grooms, D. L., Alocilja, E. C., & Bolin, S. R. (2008) · Sensors · doi:10.3390/s8096015 · Cited by 25 · see citing works
The undiscovered country: Ecotoxicology meets microfluidics
Campana, O., & Włodkowic, D. (2017) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2017.11.002 · Cited by 25 · see citing works
Microalgal photosynthetic activity measurement system for rapid toxicity assessment
Cho, C.-W., Pham, T. P. T., Jeon, Y., Min, J., Jung, H. Y., Lee, D. S., & Yun, Y. (2008) · Ecotoxicology · doi:10.1007/s10646-008-0197-x · Cited by 24 · see citing works
Early Warning Strategies and Practices Along the River Rhine
Diehl, P., Gerke, T., Jeuken, A., Lowis, J., Steen, R., van Steenwijk, J., Stoks, P. G., & Willemsen, H. (2005) · The handbook of environmental chemistry. Volume 5 · doi:10.1007/698_5_015 · Cited by 22 · see citing works
Environmental monitoring of urban streams using a primary fish gill cell culture system (FIGCS)
Schnell, S., Bawa-Allah, K. A., Otitoloju, A. A., Högstrand, C., Miller, T. H., Barron, L., & Bury, N. R. (2015) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2015.06.012 · Cited by 21 · see citing works
Endo, R., & Omasa, K. (2004) · Environmental Science & Technology · doi:10.1021/es035375+ · Cited by 21 · see citing works
Biosensors: Potentiometric and Amperometric
BORMAN, S. (1987) · Analytical Chemistry · doi:10.1021/ac00145a727 · Cited by 21 · see citing works
Application of algae-biosensor for environmental monitoring
Umar, L., Alexander, F. A., & Wiest, J. (2015) · doi:10.1109/embc.2015.7320028 · Cited by 20 · see citing works
Algal biosensor array on a single electrode
Tatsuma, T., Yoshida, Y., Shitanda, I., & Notsu, H. (2008) · The Analyst · doi:10.1039/b819040b · Cited by 15 · see citing works
Woutersen, M., van der Gaag, B., Boakye, A. A., Mink, J., Marks, R. S., Wagenvoort, A. J., Ketelaars, H. A. M., Brouwer, B. A., & Heringa, M. B. (2017) · Sensors · doi:10.3390/s17112682 · Cited by 15 · see citing works
Multiparameter Analysis of Apoptosis Using Lab‐on‐a‐Chip Flow Cytometry
Włodkowic, D., Skommer, J., Akagi, J., Fujimura, Y., & Takeda, K. (2013) · Current Protocols in Cytometry · doi:10.1002/0471142956.cy0942s66 · Cited by 13 · see citing works
Zhang, G., Chen, L., Liu, Y., Chon, T., Ren, Z., Wang, Z., Zhao, J., & Zhao, Y. (2011) · Water Science & Technology · doi:10.2166/wst.2011.750 · Cited by 13 · see citing works
Brennan, L. M., Widder, M. W., McAleer, M. K., Mayo, M. W., Greis, A. P., & van der Schalie, W. H. (2016) · Journal of Visualized Experiments · doi:10.3791/53555 · Cited by 13 · see citing works
Analysis of Mathematical Modelling on Potentiometric Biosensors
Mehala, N., & Rajendran, L. (2014) · ISRN Biochemistry · doi:10.1155/2014/582675 · Cited by 12 · see citing works
Anandraj, A., White, S., Naidoo, D., & Mutanda, T. (2020) · Bioresource Technology · doi:10.1016/j.biortech.2020.123380 · Cited by 12 · see citing works
Dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants
Yaliang, S., Ticho, Z., Jianli, G., & Li, Y. (2004) · Current Medical Science · doi:10.1007/bf02831122 · Cited by 11 · see citing works
A novel approach for phytotoxicity assessment by CCD fluorescence imaging
Gavel, A., & Maršálek, B. (2004) · Environmental Toxicology · doi:10.1002/tox.20043 · Cited by 11 · see citing works
Investigations to extend viability of a rainbow trout primary gill cell culture
Maunder, R. J., Baron, M. G., Owen, S. F., & Jha, A. N. (2017) · Ecotoxicology · doi:10.1007/s10646-017-1856-6 · Cited by 11 · see citing works
The impedance method for monitoring total coliforms in wastewaters
Strauss, W. M., Malaney, G. W., & Tanner, R. D. (1984) · Folia Microbiologica · doi:10.1007/bf02872933 · Cited by 9 · see citing works
Brennan, L. M., Widder, M. W., McAleer, M. K., et al. (2016) · Journal of Visualized Experiments · doi:10.3791/53555-v · Cited by 5 · see citing works
[The algae toximeter for continuous water monitoring].
Noack, U., & Walter, J. (1992) · PubMed · Cited by 4 · see citing works
[A biotest for water monitoring based on delayed fluorescence of algae].
Gerhardt, & Putzger, J. (1992) · PubMed · Cited by 2 · see citing works
Dark Variants of Luminous Bacteria Whole Cell Bioluminescent Optical Fiber Sensor to Genotoxicants
孙雅量, 周铁波, 过健俐, et al. (2004) · 华中科技大学学报:医学英德文版 · Cited by 1 · see citing works
Flagged first, then by citations
2 of the 153 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- Gerhardt, 2020, Online Biomonitoring for integrated smart real-time water management, Water Solut., 3, 20
- Matejczyk, 2010, The potential of application of microbial biosensors, Postep. Mikrobiol., 49, 297
Cited by
Sorted by citations
Progress on microalgae cultivation in wastewater for bioremediation and circular bioeconomy
Satya, A. D. M., Cheah, W. Y., Yazdi, S. K., Cheng, Y., Khoo, K. S., Vo, D. N., Bui, X. D., Vithanage, M. S., & Show, P. L. (2022) · Environmental Research · doi:10.1016/j.envres.2022.114948 · Cited by 108 · see citing works
Virk, V., Deepak, H., Taneja, K., Srivastava, R., & Giri, S. (2024) · Frontiers in Nanotechnology · doi:10.3389/fnano.2024.1310165 · Cited by 50 · see citing works
Dębowski, M., Kazimierowicz, J., & Zieliński, M. (2025) · Applied Sciences · doi:10.3390/app15137324 · Cited by 30 · see citing works
Włodkowic, D., & Jansen, M. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.135929 · Cited by 26 · see citing works
Biomonitoring for Watershed Protection from a Multiscale Land-Use Perspective
de Mello, K., Taniwaki, R. H., Macedo, D. R., Leal, C. G., & Randhir, T. O. (2023) · Diversity · doi:10.3390/d15050636 · Cited by 25 · see citing works
Coronado-Apodaca, K. G., González-Meza, G. M., Aguayo-Acosta, A., Araújo, R. G., González-González, R. B., Oyervides-Muñoz, M. A., Martínez-Ruiz, M., Melchor-Martínez, E. M., Barceló, ., Parra‐Saldívar, R., et al. (2023) · Topics in Catalysis · doi:10.1007/s11244-023-01786-8 · Cited by 22 · see citing works
Zeeshan, Bahrami, S., Park, S., & Cho, S. (2024) · Talanta · doi:10.1016/j.talanta.2024.125831 · Cited by 18 · see citing works
Smart sensors and AI: Sustainable solutions for environmental pollution challenges
Mutlag, F., Elaibi, H. K., Mahious, R., Halvaci, E., & Şen, F. (2025) · Next research. · doi:10.1016/j.nexres.2025.101195 · Cited by 13 · see citing works
Sahu, S., Kaur, A., Singh, G., & Arya, S. K. (2024) · Energy Ecology and Environment · doi:10.1007/s40974-024-00349-1 · Cited by 12 · see citing works
Gaertner, K., Michell, C., Tapanainen, R., Goffart, S., Saari, S., Soininmäki, M., Dufour, É., & Pohjoismäki, J. (2022) · Molecular Ecology · doi:10.1111/mec.16755 · Cited by 12 · see citing works
Kizgin, A., Schmidt, D., Bosshard, J., Singer, H., Hollender, J., Morgenroth, E., Kienle, C., & Langer, M. (2024) · Environmental Science & Technology · doi:10.1021/acs.est.4c07316 · Cited by 11 · see citing works
Advances in biomonitoring of streams and rivers
Masese, F. O., Iteba, J. O., Wanderi, E. W., Ngodhe, S. O., Mpopetsi, P. P., & Dalu, T. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-23898-7.00027-0 · Cited by 11 · see citing works
Overview and Perspectives of Biomonitoring in the Global South
Izah, S. C., Abdelkhalek, S. T., Ogwu, M. C., & Hamidifar, H. (2024) · Springer eBooks · doi:10.1007/978-981-97-1658-6_1 · Cited by 10 · see citing works
Deepa, A., Mastan, A., & Viswanath, B. (2025) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2025.118227 · Cited by 8 · see citing works
Rani, D., Rana, V., Rani, A., Malyan, S. K., Kumar, A., Dhaka, R. K., & Rana, A. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-91023-1.00009-0 · Cited by 8 · see citing works
Mortazavi, S., Makouei, S., Abbasian, K., & Danishvar, S. (2025) · Photonics · doi:10.3390/photonics12111136 · Cited by 6 · see citing works
Multiplex sensing of chlorine species in water: challenges and future perspectives: A mini-review
Diab, M. A., Hassan, W. H., Shah, M., El-Sabban, H. A., & Baek, K.-H. (2025) · Microchemical Journal · doi:10.1016/j.microc.2025.115491 · Cited by 4 · see citing works
Environmental Diagnosis through a Flow Cytometric Approach
Panza, G., Frontalini, F., Ciacci, C., Protano, G., Montanari, M., Lopez, D., Nannoni, F., Papa, S., Ortolani, C., Rebecchi, F., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms252011069 · Cited by 4 · see citing works
Luminescence-Based Sensors for Water Quality Analysis
Orellana, G., Darder, M. D. M., & Quílez-Alburquerque, J. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-822548-6.00116-3 · Cited by 4 · see citing works
Blockchain Technology and Sanitary Aspects of the Water Supply
Computer Science, I. S. A. T., Herzog, D. J., & Herzog, N. J. (2024) · Archives of Case Reports Open Access · doi:10.33140/acroa.01.01.02 · Cited by 3 · see citing works
Huang, Y., Wu, J., Lu, Y., Wang, R., Lan, Y., & Jia, N. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.168943 · Cited by 1 · see citing works
Antibiotics Induce Metabolic and Physiological Responses in Daphnia magna
O’Rourke, K., Antepowicz, I., Engelmann, B., Rolle-Kampczyk, U. E., von Bergen�, M., & Grintzalis, K. (2026) · Water · doi:10.3390/w18020265 · Cited by 1 · see citing works
Nanobiosensors for Precision Plant Disease Management
Elsharkawy, M. M. (2025) · Sustainability Sciences in Asia and Africa · doi:10.1007/978-981-96-8335-2_7 · Cited by 1 · see citing works
Freshwater Quality Monitoring with Biota - Technical Guidance Document
United Nations Environment Programme (2024) · United Nations Environment Programme eBooks · doi:10.59117/20.500.11822/44817
Utilization of marine microbial products for the creation of biosensors
Vyshnava, S. S., Swetha, K., Pandluru, G., Panjala, S. P., Paramasivam, K., Krishnamoorthy, D., & Srinivasulu, M. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-43836-3.00007-0
Engineered Microalgae: A Sustainable Solution for the Remediation of Organic Contaminants
Roy, A., Kiran, B. R., & Poluri, K. M. (2026) · Springer eBooks · doi:10.1007/978-981-95-7234-2_14
Prządka, M. P., Obstarczyk, A., Chodasewicz, P., Baluta, S., Kapuścik, P., Poręba, P., Pokora, P., Kowalska, E., Domaradzki, J., Mazur, M., et al. (2026) · Applied Surface Science · doi:10.1016/j.apsusc.2026.168384
- Authors
- Wlodkowic, D., & Karpiński, T. M.
- Affiliations
- The Neurotox Laboratory, School of Science, RMIT University, Plenty Road, P.O. Box 71, Bundoora, VIC 3083, Australia
- Poznan University of Medical Sciences (PL)
- Venue
- Sensors
- Publisher
- MDPI AG
- Published
- 2021-10-23
- Volume
- 21
- Issue
- 21
- Pages
- 7028
- ISSN
- 1424-8220 (ISSN-L)
- PMC
- PMC8588540
- OpenAlex
- W3208604774
- Cited by
- 28
- References
- 153
- Citations by year
- 2026: 5 · 2025: 6 · 2024: 10 · 2023: 3 · 2022: 4
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Sensors (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RMIT Research Repository (RMIT University Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion, cc-by)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Physical Sciences › Environmental Science › Water Science and Technology › Water Quality Monitoring Technologies
- Keywords
- water pollution monitoring, biological early warning systems, water quality, chemical contaminants, cyanobacteria, microalgae, bacteria, cell immobilization, lab-on-a-chip, recombinant microorganisms, real-time detection, aquatic organisms
- MeSH terms
- Biological Monitoring, Environmental Monitoring, Environmental Pollutants, Humans, Water Pollutants, Chemical, Water Pollution, Biosensing Techniques, Water Quality
About these checks
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