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Cited by 5Immunotherapy and Immune ResponsesT-cell and B-cell ImmunologyCancer Research and Treatments

Effects of dendritic cells from cord blood CD34+cells on human hepatocarcinoma cell line BEL-7402 in vitro and in SCID mice

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Su, Zhong-Jing·2005·World Journal of Gastroenterology
DOI 10.3748/wjg.v11.i16.2502PMC PMC4305643
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Abstract

AIM: To develop a cancer vaccine of dendritic cells derived from human cord blood CD34+ cells and to investigate its cytotoxicity on human hepatocarcinoma cells in vitro and in severe combined immunodeficiency (SCID) mice. METHODS: Lymphocytes from cord blood or peripheral blood were primed by DCs, which were derived from cord blood and pulsed with whole tumor cell lysates. Nonradiative neutral red uptake assay was adopted to detect the cytotoxicity of primed lymphocytes on human hepatocarcinoma cell line BEL-7402 in vitro. The anti-tumor effect of primed lymphocytes in vivo was detected in SCID mice, including therapeutic effect and vaccination effect. RESULTS: The cytotoxicity of DC vaccine primed lymphocytes from cord blood or peripheral blood on human hepatocarcinoma cell line BEL-7402 was significantly higher than that of unprimed lymphocytes in vitro (44.09% vs 14.69%, 47.92% vs 19.44%, P<0.01). There was no significant difference between the cytotoxicity of primed lymphocytes from cord blood and peripheral blood (P>0.05). The tumor growth rate and tumor size were smaller in SCID mice treated or vaccinated with primed lymphocytes than those with unprimed lymphocytes. SCID mice vaccinated with primed lymphocytes had a lower tumor incidence (80% vs 100%, P<0.05) and delayed tumor latent period compared with mice vaccinated with unprimed lymphocytes (11 d vs 7 d, P<0.01). CONCLUSION: Vaccine of cord blood derived-DCs has an inhibitory activity on growth of human hepatocarcinoma cells in vitro and in SCID mice. The results also implicate the potential role of cord blood derived-DC vaccine in clinical tumor immunotherapy.

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Authors
Su, Z.-J.
Venue
World Journal of Gastroenterology
Publisher
Baishideng Publishing Group Inc.
Published
2005
Volume
11
Issue
16
Pages
2502
ISSN
1007-9327 (ISSN-L)
DOI
10.3748/wjg.v11.i16.2502
PMC
PMC4305643
OpenAlex
W2150616722
Cited by
5
References
26
Citations by year
2023: 1 · 2015: 1
FWCI
0.24
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • World Journal of Gastroenterology (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Shantou University
  • Shantou University Medical College
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
Keywords
dendritic cells, cord blood CD34+ cells, dendritic cell vaccine, hepatocellular carcinoma, BEL-7402 cells, cancer immunotherapy, cytotoxicity, tumor growth inhibition, tumor latency, tumor incidence, SCID mice, lymphocyte priming
MeSH terms
Animals, Dendritic Cells, Fetal Blood, Carcinoma, Hepatocellular, Humans, Liver Neoplasms, Lymphocytes, Mice, SCID, Antigens, CD34, Cancer Vaccines, Xenograft Model Antitumor Assays, Cell Line, Tumor, Mice

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