The fundamentals of eye tracking part 6: working with areas of interest
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Researchers use area of interest (AOI) analyses to interpret eye-tracking data. This article addresses four key aspects of AOI use: 1) how to report AOIs to support replicable analyses, 2) how to interpret AOI-related statistics, 3) methods for generating both static and dynamic AOIs, and 4) recent developments and future directions in AOI use. The article underscores the importance of aligning AOI design with the study’s conceptual and methodological foundations. It argues that critical decisions, such as the size, shape, and placement of AOIs, should be made early in the experimental design process and should involve eye-tracking data quality, the research question, participant tasks, and the nature of the visual stimulus. It also evaluates recent advances in AOI automation, outlining both their benefits and limitations. The article’s main message is that researchers should plan AOIs carefully and explain their choices openly so others can replicate the work.
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The AOI classification enabled attention to equivalent information functions to be compared despite differences in page layout and interface structure.
“This classification enabled attention to equivalent information functions to be compared despite differences in page layout and interface structure (Hooge et al., 2026).”
From Responsible use of generative AI in Chinese vocational education: eye-tracking evidence on source verification and immediate near-transfer across three information-seeking conditions (2026) · cites this paper as Hooge et al., 2026
In ‘Static AOIs > Deciding on the AOI’ (page not provided), the paper describes defining AOIs by information category and discusses comparing the same objects across different images or displays. It does not establish that the citing authors’ classification enabled comparison of equivalent information functions despite differences in page layout and interface structure. (LLM, conf=0.79)
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Kirillov, A., Mintun, E., Ravi, N., Mao, H., Rolland, C., Gustafson, L., Xiao, T., Whitehead, S., Berg, A. C., Lo, W.-Y., et al. (2023) · 2023 IEEE/CVF International Conference on Computer Vision (ICCV) · doi:10.1109/iccv51070.2023.00371 · Cited by 11,953 · cited 1× · see citing works
“7B, have since been extended using the Segment Anything Model (SAM, Kirillov et al., 2023) and the Computer Vision Annotation Tool (CVAT, https://www.cvat.ai).”
Attention to eyes is present but in decline in 2–6-month-old infants later diagnosed with autism
Jones, W., & Klin, A. (2013) · Nature · doi:10.1038/nature12715 · Cited by 1,293 · cited 1× · see citing works
“Repeating an AOI analysis might become more challenging if the original study involves hand-drawn AOIs with real-world stimulus material, such as faces (e.g., Falck-Ytter, 2008; Jones & Klin, 2013), landscapes (Zhou et al., 2022) or newspapers (Wartenberg & Holmqvist, 2005).”
Gaze bias both reflects and influences preference
Shimojo, S., Simion, C., Shimojo, E., & Scheier, C. (2003) · Nature Neuroscience · doi:10.1038/nn1150 · Cited by 924 · see citing works
Cornelissen, T. H. W., & Võ, M. L.-H. (2017) · Attention, Perception, & Psychophysics · doi:10.3758/s13414-016-1203-7 · Cited by 70 · see citing works
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Cited by
Sorted by citations
Ma, J., Yue, H., & Li, X. (2026) · Frontiers in Psychology · doi:10.3389/fpsyg.2026.1946196
- Authors
- Hooge, I. T. C., Nyström, M., Niehorster, D. C., Andersson, R., Foulsham, T., Nuthmann, A., & Hessels, R. S.
- Affiliations
- Utrecht University (NL)
- Lund University (SE)
- Tobii (Sweden) (SE)
- University of Essex (GB)
- Christian-Albrechts-Universität zu Kiel (DE)
- Corresponding author
- Hooge, Ignace T. C.
- ORCID
- Venue
- Behavior Research Methods
- Published
- 2026-02-17
- Volume
- 58
- Issue
- 3
- Pages
- 65-65
- ISSN
- 1554-351X (ISSN-L)
- 1554-3528
- PMC
- PMC12913287
- OpenAlex
- W7129678306
- Cited by
- 22
- References
- 4
- Citations by year
- 2026: 22
- FWCI
- 110.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Behavior Research Methods (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Lund University Publications (Lund University) (submittedVersion)
- Multimedialen Archiv und Publikationsserver der Christian-Albrechts-Universität zu Kiel (Christian-Albrechts-Universität zu Kiel) (submittedVersion, cc-by)
- Topics
- Physical Sciences › Computer Science › Human-Computer Interaction › Gaze Tracking and Assistive Technology
- Physical Sciences › Computer Science › Computer Vision and Pattern Recognition › Visual Attention and Saliency Detection
- Life Sciences › Neuroscience › Cognitive Neuroscience › Visual perception and processing mechanisms
- Keywords
- eye tracking, areas of interest, replicability, experimental design
- MeSH terms
- Eye-Tracking Technology, Eye Movements, Humans, Research Design
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