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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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“Observation of a mixture of early and late autophagic vacuoles that is modulated by the time point of collection and/or brief pulses of bafilomycin A1 to trap the cargo in a recognizable early state [56] increases the confidence that an autophagic process is being observed.”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [56]
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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., Abeliovich, H., Acevedo Arozena, A., Adachi, H., Adams, C. M., Adams, P. D., Adeli, K., et al. (2016) · Autophagy · doi:10.1080/15548627.2015.1100356 · Cited by 5,894
Self-citationA highly potent and selective Vps34 inhibitor alters vesicle trafficking and autophagy
Ronan, B., Flamand, O., Vescovi, L., Dureuil, C., Durand, L., Fassy, F., Bachelot, M.-F., Lamberton, A., Mathieu, M., Bertrand, T., et al. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1681 · Cited by 500
Fass, E., Shvets, E., Degani, I., Hirschberg, K., & Elazar, Z. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m607031200 · Cited by 286
Prelysosomal convergence of autophagic and endocytic pathways
Gordon, P. B., & Seglen, P. O. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(88)90556-6 · Cited by 281 · cited 1×
“Using these assays, Seglen defined and characterized different steps of the autophagic-lysosomal pathway 4,5 , discovered and coined the amphisome 6 (the product of endosome-autophagosome fusion), and was the first to describe the role of protein phosphorylation in autophagy regulation 7 .”
MethodSystematic analysis of ribophagy in human cells reveals bystander flux during selective autophagy
An, H., & Harper, J. W. (2018) · Nature Cell Biology · doi:10.1038/s41556-017-0007-x · Cited by 181
Rosado, C., Mijaljica, D., Hatzinisiriou, I., Prescott, M., & Devenish, R. J. (2008) · Autophagy · doi:10.4161/auto.5331 · Cited by 140
Modulation of intracellular calcium homeostasis blocks autophagosome formation
Engedal, N., Torgersen, M. L., Guldvik, I. J., Barfeld, S. J., Bakula, D., Sætre, F., Hagen, L. K., Patterson, J. B., Proikas-Cezanne, T., Seglen, P. O., et al. (2013) · Autophagy · doi:10.4161/auto.25900 · Cited by 104
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Research works printed but not linked: 37
- Rubinsztein, D. C., & Frake, R. A. Yoshinori Ohsumi's Nobel Prize for mechanisms of autophagy: from basic yeast biology to therapeutic potential. J R Coll Physicians Edinb. 46 (4), 228-233, (2016). · DOI
- Mizushima, N. The exponential growth of autophagy-related research: from the humble yeast to the Nobel Prize. FEBS Lett. 591 (5), 681-689, (2017). · DOI
- Seglen, P. O. et al. Macroautophagic cargo sequestration assays. Methods. 75 25-36, (2015). · DOI
- Høyvik, H., Gordon, P. B., & Seglen, P. O. Use of a hydrolysable probe, [14C]lactose, to distinguish between pre-lysosomal and lysosomal steps in the autophagic pathway. Exp Cell Res. 166 (1), 1-14, (1986). · DOI
- Plomp, P. J., Gordon, P. B., Meijer, A. J., Hoyvik, H., & Seglen, P. O. Energy dependence of different steps in the autophagic-lysosomal pathway. J Biol Chem. 264 (12), 6699-6704, (1989). · DOI
- Holen, I., Gordon, P. B., & Seglen, P. O. Protein kinase-dependent effects of okadaic acid on hepatocytic autophagy and cytoskeletal integrity. Biochem J. 284 (Pt 3) 633-636, (1992). · DOI
- Kabeya, Y. et al. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. Embo j. 19 (21), 5720-5728, (2000). · DOI · Paper page
- Szalai, P. et al. Autophagic bulk sequestration of cytosolic cargo is independent of LC3, but requires GABARAPs. Exp Cell Res. 333 (1), 21-38, (2015). · DOI · Paper page
- Nguyen, T. N. et al. Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. J Cell Biol. 215 (6), 857-874, (2016). · DOI · Paper page
- Pontano Vaites, L., Paulo, J. A., Huttlin, E. L., & Harper, J. W. Systematic analysis of human cells lacking ATG8 proteins uncovers roles for GABARAPs and the CCZ1/MON1 regulator C18orf8/RMC1 in macro and selective autophagic flux. Mol Cell Biol. 10.1128/mcb.00392-17, (2017). · DOI · Paper page
- Kopitz, J., Kisen, G. O., Gordon, P. B., Bohley, P., & Seglen, P. O. Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes. J Cell Biol. 111 (3), 941-953, (1990). · DOI
- Bowman, E. J., Siebers, A., & Altendorf, K. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc Natl Acad Sci U S A. 85 (21), 7972-7976, (1988). · DOI
- Hadjivassiliou, A. G., & Rieder, S. V. The enzymatic assay of pyruvic and lactic acids. A definitive procedure. Clin Chim Acta. 19 (3), 357-361, (1968). · DOI
- Bergmeyer, H. U., & Bernt, E. in Methods of enzymatic analysis (2nd English ed.). Vol. 2 (ed H.U. Bergmeyer) 574-579 Verlag Chemie, (1974).
- Luhr, M. et al. A Simple Cargo Sequestration Assay for Quantitative Measurement of Nonselective Autophagy in Cultured Cells. Methods Enzymol. 587 351-364, (2017). · DOI
- Mousavi, S. A. et al. Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy. Biochim Biophys Acta. 1510 (1-2), 243-257, (2001). · DOI
- Seglen, P. O., & Gordon, P. B. 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc Natl Acad Sci U S A. 79 (6), 1889-1892, (1982). · DOI
- Sætre, F., Hagen, L. K., Engedal, N., & Seglen, P. O. Novel steps in the autophagic-lysosomal pathway. Febs j. 282 (11), 2202-2214, (2015). · DOI · Paper page
- Seglen, P. O., Øverbye, A., & Sætre, F. Sequestration assays for mammalian autophagy. Methods Enzymol. 452 63-83, (2009). · DOI · Paper page
- Hurley, J. H., & Young, L. N. Mechanisms of Autophagy Initiation. Annu Rev Biochem. 86 225-244, (2017). · DOI
- Thastrup, O., Cullen, P. J., Drobak, B. K., Hanley, M. R., & Dawson, A. P. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2(+)-ATPase. Proc Natl Acad Sci U S A. 87 (7), 2466-2470, (1990). · DOI
- Kuma, A. et al. The role of autophagy during the early neonatal starvation period. Nature. 432 (7020), 1032-1036, (2004). · DOI · Paper page
- Komatsu, M. et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol. 169 (3), 425-434, (2005). · DOI · Paper page
- Saitoh, T. et al. Atg9a controls dsDNA-driven dynamic translocation of STING and the innate immune response. Proc Natl Acad Sci U S A. 106 (49), 20842-20846, (2009). · DOI · Paper page
- Gordon, P. B., & Seglen, P. O. Autophagic sequestration of [14C]sucrose, introduced into rat hepatocytes by reversible electro-permeabilization. Exp Cell Res. 142 (1), 1-14, (1982). · DOI
- Velikkakath, A. K., Nishimura, T., Oita, E., Ishihara, N., & Mizushima, N. Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Mol Biol Cell. 23 (5), 896-909, (2012). · DOI
- Øverbye, A., Sætre, F., Hagen, L. K., Johansen, H. T., & Seglen, P. O. Autophagic activity measured in whole rat hepatocytes as the accumulation of a novel BHMT fragment (p10), generated in amphisomes by the asparaginyl proteinase, legumain. Autophagy. 7 (9), 1011-1027, (2011). · DOI · Paper page
- Kominami, E., Hashida, S., Khairallah, E. A., & Katunuma, N. Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin. J Biol Chem. 258 (10), 6093-6100, (1983). · DOI
- Katayama, H., Kogure, T., Mizushima, N., Yoshimori, T., & Miyawaki, A. A sensitive and quantitative technique for detecting autophagic events based on lysosomal delivery. Chem Biol. 18 (8), 1042-1052, (2011). · DOI · Paper page
- Ogier-Denis, E. et al. A heterotrimeric Gi3-protein controls autophagic sequestration in the human colon cancer cell line HT-29. J Biol Chem. 270 (1), 13-16, (1995). · DOI
- Seglen, P. O., Gordon, P. B., Tolleshaug, H., & Høyvik, H. Use of [3H]raffinose as a specific probe of autophagic sequestration. Exp Cell Res. 162 (1), 273-277, (1986). · DOI
- Luhr, M., Sætre, F., & Engedal, N. The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent Cell Lines. Bio-protocol. 8 (9), e2836, (2018). · DOI · Paper page
- Rønning, O. W., Pettersen, E. O., & Seglen, P. O. Protein synthesis and protein degradation through the cell cycle of human NHIK 3025 cells in vitro. Exp Cell Res. 123 (1), 63-72, (1979). · DOI
- Seglen, P. O., Grinde, B., & Solheim, A. E. Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes by ammonia, methylamine, chloroquine and leupeptin. Eur J Biochem. 95 (2), 215-225, (1979). · DOI
- Seglen, P. O., & Solheim, A. E. Valine uptake and incorporation into protein in isolated rat hepatocytes. Nature of the precursor pool for protein synthesis. Eur J Biochem. 85 (1), 15-25, (1978). · DOI
- Bauvy, C., Meijer, A. J., & Codogno, P. Assaying of autophagic protein degradation. Methods Enzymol. 452 47-61, (2009). · DOI
- Engedal, N., & Seglen, P. O. Autophagy of cytoplasmic bulk cargo does not require LC3. Autophagy. 12 (2), 1-3, (2016). · DOI · Paper page
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
Klionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,897
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Metadata
- Authors
- Luhr, M., Szalai, P., & Engedal, N.
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2018-07-27
- Issue
- 137
- ISSN
- 1940-087X
- Cited by
- 7
- References
- 7
- Language
- en
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