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Karyotypic variation between independently cultured strains of the cell line MCF-7 identified by multicolour fluorescence in situ hybridization
Abstract
The breast cancer cell line MCF-7 is a widely used model in breast cancer research however a number of conflicting reports have been published regarding its biological properties. We hypothesised that there will be significant in vitro mutation and genotypic evolution over time in this cell line. To assess the genetic divergence of MCF-7 at the chromosomal level, we analysed MCF-7 cell lines grown independently at three different laboratories using M-FISH and CGH. In addition, MCF-7 cells from our own laboratory were also analysed at two time points 18 months apart. Several common chromosomal translocations were identified in all variants of the cell lines. In addition, a significant number of unique abnormalities were identified, characterising each of the variants studied. Genotypic differences between cell lines grown independently in different laboratories would significantly alter the phenotypic characteristics of each cell line rendering biological properties inconsistent between laboratories.
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“But such heterogeneity can most certainly be a curse when it comes to other common applications of such cells, as it not only weakens “the direct relevance of such cultures as models of human cancer”8 but also “makes inter-laboratory reproduction of experimental findings difficult”24.”
From Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function (2016) · cites this paper as [24]
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Kleensang, A., Vantangoli, M. M., Odwin-DaCosta, S., Andersen, M. E., Boekelheide, K., Bouhifd, M., Fornace, A. J., Li, H.-H., Livi, C. B., Madnick, S., et al. (2016) · Scientific Reports · doi:10.1038/srep28994 · Cited by 114
Common origins of MDA-MB-435 cells from various sources with those shown to have melonoma properties
Rae, J. M., Ramus, S. J., Waltham, M., Armes, J. E., Campbell, I. G., Clarke, R., Barndt, R. J., Johnson, M. D., & Thompson, E. W. (2004) · Clinical & Experimental Metastasis · doi:10.1007/s10585-004-3759-1 · Cited by 74
Metadata
- Authors
- Bahia, H., Ashman, J., Cawkwell, L., Lind, M., Monson, J., Drew, P., & Greenman, J.
- Affiliations
- University of Hull (GB)
- Venue
- International Journal of Oncology
- Publisher
- Spandidos Publications
- Published
- 2002-03-01
- Volume
- 20
- Issue
- 3
- Pages
- 489-94
- ISSN
- 1019-6439 (ISSN-L)
- 1791-2423
- OpenAlex
- W2013373613
- Cited by
- 47
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 3 · 2020: 2 · 2019: 2 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 3
- FWCI
- 2.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Oncology (publishedVersion)
- PubMed (publishedVersion)
- Repository@Hull (Worktribe) (University of Hull) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomic variations and chromosomal abnormalities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- MCF-7 cells, karyotype variation, chromosomal translocation, breast cancer cell lines, inter-laboratory variability, chromosomal abnormalities, cell line heterogeneity
- MeSH terms
- Breast Neoplasms, Chromosome Aberrations, Chromosomes, Female, Genotype, Humans, Karyotyping, Metaphase, Nucleic Acid Hybridization, Time Factors, Translocation, Genetic, Tumor Cells, Cultured, In Situ Hybridization, Fluorescence
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- The paper
- Bahia, H., Ashman, J., Cawkwell, L., Lind, M., Monson, J., Drew, P., & Greenman, J. (2002). Karyotypic variation between independently cultured strains of the cell line MCF-7 identified by multicolour fluorescence in situ hybridization. International Journal of Oncology. https://doi.org/10.3892/ijo.20.3.489
