Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function

Kleensang, Andre; Vantangoli, Marguerite M.; Odwin-DaCosta, Shelly; Andersen, Melvin E.; Boekelheide, Kim; Bouhifd, Mounir; Fornace, Albert J.; Li, Heng-Hong; Livi, Carolina B.; Madnick, Samantha; Maertens, Alexandra; Rosenberg, Michael; Yager, James D.; Zhao, Liang; Hartung, Thomas

doi:10.1038/srep28994

Genetic variability in a frozen batch of MCF-7 cells invisible in routine authentication affecting cell function

Common recommendations for cell line authentication, annotation and quality control fall short addressing genetic heterogeneity. Within the Human Toxome Project, we demonstrate that there can be marked cellular and phenotypic heterogeneity in a single batch of the human breast adenocarcinoma cell…