Effects of the combination of dexamethasone and fibroblast growth factor-2 on differentiation of osteoprecursor cells
Open accessAbstract
Dexamethasone is a potent modulator of osteogenic differentiation and previous studies have shown that dexamethasone increases the bone formation capacity of osteoprogenitor cells in vitro. Fibroblast growth factor‑2 (FGF‑2) is reported to have numerous biological activities, including stimulation of cell growth, migration, angiogenesis, wound healing, tissue repair, differentiation and morphogenesis. In the present study, the dose‑dependent effect of dexamethasone and FGF‑2 on the differentiation of osteoprecursor cells was evaluated. The alkaline phosphatase (ALP) activity test was performed to assess differentiation and protein expression associated with bone formation was measured by western blot analysis. The results showed that the protein content of the cultures grown with the osteogenic differentiation media in the presence of 2 ng/ml FGF‑2 was increased compared with that of the untreated control. ALP activity was increased when cells were treated with dexamethasone, with the highest value at 100 nM. The addition of 20 ng/ml FGF‑2 to 100 nM dexamethasone showed an increase in ALP activity, however, this was not statistically significant. The combination of 100 nM dexamethasone and 20 ng/ml FGF‑2 produced the highest expression of bone morphogenetic protein receptor‑IA and bone morphogenetic protein receptor‑II, leading to the highest value of pSmad1/5/8 expression. Within the limits of this study, dexamethasone significantly enhanced osteoblast differentiation, however, the combined delivery of dexamethasone and FGF‑2 did not produce synergistic effects on osteoblast differentiation under the current experimental conditions.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Literature reports effective Dex concentrations for MC3T3-E1 ALP induction range from 10 to 100 nM, with optimal at 50 nM.
“Literature reports effective Dex concentrations for MC3T3-E1 ALP induction range from 10 to 100 nM [25,48,49], with optimal at 50 nM [50].”
From Functional Surface Modification of Magnesium Implant by Drug-Loaded Biodegradable Polymer Coating (2026) · cites this paper as [48]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Xiao, G., Jiang, D., Gopalakrishnan, R., & Franceschi, R. T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m206057200 · Cited by 379 · see citing works
Coelho, M. J., & Fernandes, M. H. (2000) · Biomaterials · doi:10.1016/s0142-9612(99)00192-1 · Cited by 317 · see citing works
Mansukhani, A., Bellosta, P., Sahni, M., & Basilico, C. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.149.6.1297 · Cited by 295 · see citing works
Naik, P. N., Chimatadar, S. A., & Nandibewoor, S. T. (2010) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2010.05.014 · Cited by 227 · see citing works
Fakhry, A., Ratisoontorn, C., Vedhachalam, C., Salhab, I., Koyama, E., Leboy, P., Pacifici, M., Kirschner, R. E., & Nah, H. (2005) · Bone · doi:10.1016/j.bone.2004.10.003 · Cited by 179 · see citing works
Ishida, Y., & Heersche, J. N. M. (1998) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.1998.13.12.1822 · Cited by 155 · see citing works
Atmani, H., Chappard, D., & Baslé, M. F. (2003) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10507 · Cited by 91 · see citing works
Kim, C., Cheng, S.-L., & Kim, G. (1999) · Journal of Endocrinology · doi:10.1677/joe.0.1620371 · Cited by 83 · see citing works
Beloti, M. M., & Rosa, A. L. (2005) · Brazilian Dental Journal · doi:10.1590/s0103-64402005000200013 · Cited by 80 · see citing works
Park, J. (2010) · Journal of Surgical Research · doi:10.1016/j.jss.2010.09.010 · Cited by 72 · see citing works
Self-citationSobue, T., Naganawa, T., Xiao, L., Okada, Y., Tanaka, Y., Ito, M., Okimoto, N., Nakamura, T., Coffin, J. D., & Hurley, M. M. (2005) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20389 · Cited by 68 · see citing works
Nakamura, Y., Wakitani, S., Nakayama, J., et al. (2003) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.2003.18.10.1854 · Cited by 53 · see citing works
Park, J. (2011) · Journal of Craniofacial Surgery · doi:10.1097/scs.0b013e31822e8216 · Cited by 52 · see citing works
Self-citationHakki, S. S., Nohutçu, R. M., Hakki, E. E., Berry, J. E., Akkaya, M. S., & Somerman, M. J. (2005) · Journal of Periodontology · doi:10.1902/jop.2005.76.9.1550 · Cited by 34 · see citing works
Lee, S., Lim, J., Khang, G., et al. (2009) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0465 · Cited by 29 · see citing works
Park, J. (2012) · Experimental and Therapeutic Medicine · doi:10.3892/etm.2012.590 · Cited by 24 · see citing works
Self-citationPark, J. (2012) · Journal of Surgical Research · doi:10.1016/j.jss.2012.03.072 · Cited by 21 · see citing works
Self-citationKotev‐Emeth, S., Pitaru, S., Pri‐Chen, S., et al. (2002) · Connective Tissue Research · doi:10.1080/03008200290001339 · Cited by 18 · see citing works
Park, J. (2011) · Journal of Craniofacial Surgery · doi:10.1097/scs.0b013e31822e8434 · Cited by 14 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Functional Surface Modification of Magnesium Implant by Drug-Loaded Biodegradable Polymer Coating
Park, J.-E., Jang, Y.-S., Ko, S.-O., & Lee, M.-H. (2026) · Applied Sciences · doi:10.3390/app16031542 · Cited by 2 · see citing works
Self-citation
- Authors
- PARK, J.-B.
- Affiliations
- The Catholic University of Korea Seoul St. Mary's Hospital (KR)
- Corresponding author
- PARK, JUN-BEOM
- Venue
- Molecular Medicine Reports
- Publisher
- Spandidos Publications
- Published
- 2013-11-19
- Volume
- 9
- Issue
- 2
- Pages
- 659-662
- ISSN
- 1791-2997 (ISSN-L)
- 1791-3004
- OpenAlex
- W2063062118
- Semantic Scholar
- 314360c1c6f52e0bf6533852491ffb5fa276529d
- Cited by
- 15
- References
- 19
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 3 · 2017: 1 · 2016: 2 · 2015: 1 · 2014: 1
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Medicine Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Catholic University of Korea
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Keywords
- dexamethasone, fibroblast growth factor 2, osteogenic differentiation, osteoprogenitor cells, alkaline phosphatase activity, Smad1/5/8 phosphorylation, synergistic effect, glucocorticoids, bone formation
- MeSH terms
- Alkaline Phosphatase, Animals, Cell Differentiation, Cells, Cultured, Dexamethasone, Drug Combinations, Humans, Osteogenesis, Transforming Growth Factor beta, Fibroblast Growth Factor 2, Gene Expression Regulation, Developmental, Bone Morphogenetic Proteins, Cell Proliferation, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
