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miR-128 enhances dendritic cell-mediated anti-tumor immunity via targeting of p38

Liang, Xue, Shangguan, Wenfeng, Zhang, Miaomiao, Mei, Shiyue, Wang, Liyang, Yang, Rongcun·2017·Molecular Medicine Reports
DOI 10.3892/mmr.2017.6717PMC PMC5561786
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Figure 1. Expression of miR-128 and p38 levels in DCs as a response to B16 stimulation. (A) miR-128, (B) p38 mRNA and (C) p38 protein expression levels in DCs following B16 stimulation. Difference between two groups was analyzed by t-test. *P<0.05 vs. control. miR, miRNA; DC, dendritic cells.
Figure 1. Expression of miR-128 and p38 levels in DCs as a response to B16 stimulation. (A) miR-128, (B) p38 mRNA and (C) p38 protein expression levels in DCs following B16 stimulation. Difference between two groups was analyzed by t-test. *P<0.05 vs. control. miR, miRNA; DC, dendritic cells.CC BY-NC-ND · PubMed Central
Figure 2. Effect of miR-128 on p38 expression levels in DCs. (A) Expression of p38 gene was not significantly altered by miR-128 mimic or inhibitor in DCs. (B) Effects of differing doses of miR-128 mimic (1, 10 or 100 nM) on p38 gene expression. (C) Effects of differing doses of miR-128 inhibitor (1, 10 or 100 nM) on p38 gene expression. (D) miR-128 mimic and inhibitor had markedly contrasting effects in the regulation of the expression of p38 protein. (E) Effects of differing doses of miR-128 mimic (1, 10 or 100 nM) on p38 protein. miR-128 mimic demonstrated a dose-dependent effect on the inhibition of p38 protein. (F) Effects of differing doses of miR-128 inhibitor (1, 10 or 100 nM) on p38 protein. p38 protein accumulation was enhanced by miR-128 inhibitor in a dose-dependent manner. Differences among groups were analyzed by one-way analysis of variance. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells.
Figure 2. Effect of miR-128 on p38 expression levels in DCs. (A) Expression of p38 gene was not significantly altered by miR-128 mimic or inhibitor in DCs. (B) Effects of differing doses of miR-128 mimic (1, 10 or 100 nM) on p38 gene expression. (C) Effects of differing doses of miR-128 inhibitor (1, 10 or 100 nM) on p38 gene expression. (D) miR-128 mimic and inhibitor had markedly contrasting effects in the regulation of the expression of p38 protein. (E) Effects of differing doses of miR-128 mimic (1, 10 or 100 nM) on p38 protein. miR-128 mimic demonstrated a dose-dependent effect on the inhibition of p38 protein. (F) Effects of differing doses of miR-128 inhibitor (1, 10 or 100 nM) on p38 protein. p38 protein accumulation was enhanced by miR-128 inhibitor in a dose-dependent manner. Differences among groups were analyzed by one-way analysis of variance. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells.CC BY-NC-ND · PubMed Central
Figure 3. Effects of miR-128 and p38 on the mRNA and secretion levels of cytokines IL-6, −10 and −12 in DCs. The immature DCs were transfected with miR-128 mimic or inhibitor, or p38 inhibitor. Following this, cells were treated with lipopolysaccharide to promote the maturation of DCs. The mRNA expression of genes encoding (A) IL-6, (B) IL-10 and (C) IL-12 were detected via reverse transcription-quantitative polymerase chain reaction and the secretion levels were determined by ELISA during the 24 h treated time course. Differences among groups were analyzed by one-way analysis of variance, followed by Bonferroni's multiple comparison test. *P<0.05 vs. control; #P<0.05 vs. miR-128 mimic group. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells; IL, interleukin.
Figure 3. Effects of miR-128 and p38 on the mRNA and secretion levels of cytokines IL-6, −10 and −12 in DCs. The immature DCs were transfected with miR-128 mimic or inhibitor, or p38 inhibitor. Following this, cells were treated with lipopolysaccharide to promote the maturation of DCs. The mRNA expression of genes encoding (A) IL-6, (B) IL-10 and (C) IL-12 were detected via reverse transcription-quantitative polymerase chain reaction and the secretion levels were determined by ELISA during the 24 h treated time course. Differences among groups were analyzed by one-way analysis of variance, followed by Bonferroni's multiple comparison test. *P<0.05 vs. control; #P<0.05 vs. miR-128 mimic group. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells; IL, interleukin.CC BY-NC-ND · PubMed Central
Figure 4. Role of miR-128 and p38 in the therapeutic effect of DCs. (A) Final tumor volume, (B) tumor growth plot, (C) animal survival time and (D) final tumor weight were assessed in C57BL/6 mice bearing B16 melanoma following injection with DCs harboring negative control oligonucleotide, miR-128 mimic or inhibitor, or p38 inhibitor. Differences among groups were analyzed by one-way analysis of variance, followed by Bonferroni's multiple comparison test. *P<0.05 vs. control; #P<0.05 vs. miR-128 mimic group. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells.
Figure 4. Role of miR-128 and p38 in the therapeutic effect of DCs. (A) Final tumor volume, (B) tumor growth plot, (C) animal survival time and (D) final tumor weight were assessed in C57BL/6 mice bearing B16 melanoma following injection with DCs harboring negative control oligonucleotide, miR-128 mimic or inhibitor, or p38 inhibitor. Differences among groups were analyzed by one-way analysis of variance, followed by Bonferroni's multiple comparison test. *P<0.05 vs. control; #P<0.05 vs. miR-128 mimic group. NC, negative control dsRNA that did not target any gene; miR, miRNA; DC, dendritic cells.CC BY-NC-ND · PubMed Central

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Metadata

Authors
Liang, X., Shangguan, W., Zhang, M., Mei, S., Wang, L., & Yang, R.
Venue
Molecular Medicine Reports
Publisher
Spandidos Publications
Published
2017-02
Volume
16
Issue
2
Pages
1307-1313
ISSN
  • 1791-2997
  • 1791-3004
DOI
10.3892/mmr.2017.6717
PMC
PMC5561786
Cited by
16
References
0
Language
en

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Liang, X., Shangguan, W., Zhang, M., Mei, S., Wang, L., & Yang, R. (2017). miR-128 enhances dendritic cell-mediated anti-tumor immunity via targeting of p38. Molecular Medicine Reports. https://doi.org/10.3892/mmr.2017.6717