MicroRNA-23a-3p inhibitor decreases osteonecrosis incidence in a rat model
Open accessAbstract
The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-density lipoprotein receptor-related protein 5 (LRP-5) expression was assessed by immunohistochemistry. The results demonstrated the incidence of osteonecrosis decreased in the miR-23a-3p inhibitor group compared with the miR-23a-3p mimic group (18.2% vs. 75%; P<0.05). The ratio of bone volume/total volume and trabecular thickness were significantly increased in the miR-23a-3p inhibitor group compared with the miR-23a mimic group. The expression level of LRP-5 was higher in the miR-23a-3p inhibitor group. The present study indicated that miR may provide a novel and alternative approach for understanding the mechanism underlying steroid-associated necrosis of the femoral head.
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Using a viral vector to transfect miR-23a-3p mimics or inhibitors into bone mesenchymal stem cells, then injecting them into a steroid induced femoral head necrosis model, the inhibitor group could reduce the incidence of bone necrosis in the rat model.
“Using a viral vector to transfect miR-23a-3p mimics or inhibitors into bone mesenchymal stem cells, and then injecting them into a steroid induced femoral head necrosis model, it was found that the inhibitor group could reduce the incidence of bone necrosis in the rat model [117].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [117]
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Sorted by citations
Recent advances of miR-23 in human diseases and growth development
Qian, X., Jiang, Y., Yang, Y., Zhang, Y., Xu, N., Xu, B., Pei, K., Yu, Z., & Wu, W. (2025) · Non-coding RNA Research · doi:10.1016/j.ncrna.2024.12.010 · Cited by 5 · see citing works
- Authors
- Dong, Y., Li, T., Li, Y., Ren, S., Fan, J., & Weng, X.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Affiliated Hospital of Qingdao University (CN)
- Corresponding author
- Weng, Xisheng
- Venue
- Molecular Medicine Reports
- Publisher
- Spandidos Publications
- Published
- 2017-10-17
- Volume
- 16
- Issue
- 6
- Pages
- 9331-9336
- ISSN
- 1791-2997 (ISSN-L)
- 1791-3004
- PMC
- PMC5779994
- OpenAlex
- W2765644677
- Cited by
- 17
- References
- 1
- Citations by year
- 2024: 3 · 2023: 3 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 2
- FWCI
- 2.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Molecular Medicine Reports (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81272009)
- Topics
- Health Sciences › Medicine › Orthopedics and Sports Medicine › Bone and Joint Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Oncology › Bone health and treatments
- Keywords
- avascular necrosis, steroid-induced osteonecrosis, osteonecrosis of the femoral head, bone mesenchymal stem cells, miRNA inhibitors, bone microarchitecture, bone volume/total volume, trabecular thickness
- MeSH terms
- Cancellous Bone, Animals, Disease Models, Animal, Femur Head, Male, Osteonecrosis, Bone Density, Incidence, Rats, Sprague-Dawley, MicroRNAs, X-Ray Microtomography, Mesenchymal Stem Cells
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