Cyclin A is a reliable proliferation marker in endometrial cancer cell lines.
Open accessAbstract
Cyclin A, a cell cycle regulatory protein, promotes cell proliferation and has been observed to be highly expressed in cancer and to promote tumor growth; however, its value as a marker for endometrial carcinoma has not yet been established. Accordingly, the aim of the present study was to clarify whether cyclin A can be used as a cell proliferation marker using the endometrial carcinoma cell lines Ishikawa and HEC-50B, derived from patients with low-grade and high-grade cancer, respectively. The expression of cyclin A was determined by flow cytometry using double staining with FITC and 7-AAD, and immunocytochemical staining. The results were compared to those of Ki-67, the widely used cell proliferation marker that is considered to be a prognostic marker in endometrial cancer. The flow cytometry results revealed that cyclin A expression was significantly higher in HEC-50B than in Ishikawa cells during the logarithmic growth phase. In addition, cyclin A expression was consistently higher than Ki-67 expression in the examined cell lines. Immunocytochemical staining confirmed cyclin A expression in HEC-50B and Ishikawa cells, demonstrating significantly higher expression during the logarithmic growth phase than during the stationary phase. By contrast, Ki-67 was expressed in almost 90% of the cells, irrespective of their growth state. These results indicate that cyclin A expression is significantly increased in cells with higher proliferative ability and is specifically expressed in cells that have passed the G1-S checkpoint. Therefore, cyclin A may be a reliable proliferation biomarker for endometrioid carcinoma.
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Ki-67 is highly expressed in malignant tissues and promotes cell division.
“As with Cyclin A2, Ki-67 is highly expressed in malignant tissues and promotes cell division (Sales Gil and Vagnarelli, 2018; Horie et al., 2019).”
From USP7 Is a Master Regulator of Genome Stability (2020) · cites this paper as Horie et al., 2019
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- Authors
- Horie, K., Yamamoto, H., Karube, K., Takebayashi, K., Yoshino, H., Yoshioka, H., & Watanabe, J.
- Affiliations
- Hirosaki University (JP)
- Department of Bioscience and Laboratory Medicine, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036‑8564, Japan
- Corresponding author
- Horie, Kayo
- Venue
- Oncology Letters
- Publisher
- Spandidos Publications
- Published
- 2019-03-08
- Volume
- 17
- Issue
- 5
- Pages
- 4455-4462
- ISSN
- 1792-1074 (ISSN-L)
- 1792-1082
- PMC
- PMC6447861
- OpenAlex
- W2922204959
- Cited by
- 16
- References
- 34
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2021: 2 · 2020: 6 · 2019: 1
- FWCI
- 2.45
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Oncology Letters (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Hirosaki University
- Kitasato University
- Ministry of Internal Affairs and Communications
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Endometrial and Cervical Cancer Treatments
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Breast Cancer Treatment Studies
- Keywords
- cyclin A, endometrial cancer, cell proliferation markers, Ki-67, Ishikawa cells, endometrioid carcinoma, G1/S checkpoint, cell cycle regulation, tumor markers
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