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Bmi-1 induces radioresistance in MCF-7 mammary carcinoma cells

LIU, ZHI-GANG, LIU, LI, XU, LI-HUA, YI, WEI, TAO, YA-LAN, TU, ZI-WEI, LI, MAN-ZHI, ZENG, MU-SHENG, XIA, YUN-FEI·2012·Oncology Reports
DOI 10.3892/or.2011.1615PMC PMC3583403
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Figure 1 Bmi-1 regulates radiosensitivity in MCF-7 cell line. (A) Western blot analysis performed on pMSCV control vector and expressing Bmi-1 using anti-Bmi-1, and anti-α-tubulin antibodies. (B) Increased expression of Bmi-1 enhances resistance to radiotherapy. P=0.007; ANOVA test (C) Western blot analysis performed on shBmi-1 or vector cells using anti-Bmi-1, and anti-α-tubulin antibodies. (D) Down-regulation of Bmi-1 increase the sensitivity of MCF-7 cells to irradiation. P<0.001; ANOVA test.
Figure 1 Bmi-1 regulates radiosensitivity in MCF-7 cell line. (A) Western blot analysis performed on pMSCV control vector and expressing Bmi-1 using anti-Bmi-1, and anti-α-tubulin antibodies. (B) Increased expression of Bmi-1 enhances resistance to radiotherapy. P=0.007; ANOVA test (C) Western blot analysis performed on shBmi-1 or vector cells using anti-Bmi-1, and anti-α-tubulin antibodies. (D) Down-regulation of Bmi-1 increase the sensitivity of MCF-7 cells to irradiation. P<0.001; ANOVA test.CC BY · PubMed Central
Figure 2 Bmi-1 knockout delayed DNA damage repair in MCF-7 cell line. (A) Graph showing γH2AX foci in Bmi-1-depleted MCF-7 cells vs. control 5 min later after irradiation. Original magnification, ×600. (B) γH2AX foci positive cells after different dose irradiation. Mean ± SD of three experiments are reported. (C) γH2AX foci positive cells at different time point after 5 Gy irradiation between MCF-7-control cells and MCF-7-knockout cells. *P<0.001; t-test.
Figure 2 Bmi-1 knockout delayed DNA damage repair in MCF-7 cell line. (A) Graph showing γH2AX foci in Bmi-1-depleted MCF-7 cells vs. control 5 min later after irradiation. Original magnification, ×600. (B) γH2AX foci positive cells after different dose irradiation. Mean ± SD of three experiments are reported. (C) γH2AX foci positive cells at different time point after 5 Gy irradiation between MCF-7-control cells and MCF-7-knockout cells. *P<0.001; t-test.CC BY · PubMed Central
Figure 3 Bmi-1 depletion increased sub-G1 population. (A) Analysis of cell cycle distribution by flow cytometry. (B) Bmi-1 knockdown decreases mitochondrial membrane potential. Healthy mitochondria when it aggregates signal from JC-1 is depicted in red; when mitochondrial membrane potential collapses, signal corresponds to the green. Original magnification, ×400. (C) Quantitative results of fluorescence ratio (580 nm/530 nm).
Figure 3 Bmi-1 depletion increased sub-G1 population. (A) Analysis of cell cycle distribution by flow cytometry. (B) Bmi-1 knockdown decreases mitochondrial membrane potential. Healthy mitochondria when it aggregates signal from JC-1 is depicted in red; when mitochondrial membrane potential collapses, signal corresponds to the green. Original magnification, ×400. (C) Quantitative results of fluorescence ratio (580 nm/530 nm).CC BY · PubMed Central
Figure 4 Western blot analysis of apoptosis related proteins. MCF-7-control cells and MCF-7-knockout cells were harvested on Days 0, 1, 2 and 3 after 5 Gy irradiation. Lysates were subjected to Western blot analysis with the labeled antibodies.
Figure 4 Western blot analysis of apoptosis related proteins. MCF-7-control cells and MCF-7-knockout cells were harvested on Days 0, 1, 2 and 3 after 5 Gy irradiation. Lysates were subjected to Western blot analysis with the labeled antibodies.CC BY · PubMed Central

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Citations

Cites

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Metadata

Authors
LIU, Z.-G., LIU, L., XU, L.-H., YI, W., TAO, Y.-L., TU, Z.-W., LI, M.-Z., ZENG, M.-S., & XIA, Y.-F.
Venue
Oncology Reports
Publisher
Spandidos Publications
Published
2011-12-30
Volume
27
Issue
4
Pages
1116-1122
ISSN
  • 1021-335X
  • 1791-2431
DOI
10.3892/or.2011.1615
PMC
PMC3583403
Cited by
36
References
2
Language
en

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