Poly(L-Lactide)-Degrading Enzyme Production by Actinomadura keratinilytica T16-1 in 3 L Airlift Bioreactor and Its Degradation Ability for Biological Recycle
Abstract
The optimal physical factors affecting enzyme production in an airlift fermenter have not been studied so far. Therefore, the physical parameters such as aeration rate, pH, and temperature affecting PLA-degrading enzyme production by Actinomadura keratinilytica strain T16-1 in a 3 l airlift fermenter were investigated. The response surface methodology (RSM) was used to optimize PLA-degrading enzyme production by implementing the central composite design. The optimal conditions for higher production of PLA-degrading enzyme were aeration rate of 0.43 vvm, pH of 6.85, and temperature at 46° C. Under these conditions, the model predicted a PLA-degrading activity of 254 U/ml. Verification of the optimization showed that PLA-degrading enzyme production of 257 U/ml was observed after 3 days cultivation under the optimal conditions in a 3 l airlift fermenter. The production under the optimized condition in the airlift fermenter was higher than un-optimized condition by 1.7 folds and 12 folds with un-optimized medium or condition in shake flasks. This is the first report on the optimization of environmental conditions for improvement of PLA-degrading enzyme production in a 3 l airlift fermenter by using a statistical analysis method. Moreover, the crude PLA-degrading enzyme could be adsorbed to the substrate and degraded PLA powder to produce lactic acid as degradation products. Therefore, this incident indicates that PLA-degrading enzyme produced by Actinomadura keratinilytica NBRC 104111 strain T16-1 has a potential to degrade PLA to lactic acid as a monomer and can be used for the recycle of PLA polymer.
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A variety of soil microorganisms have been isolated and proved efficient for mineralization of PLLA on agar plates or in growing medium.
“A variety of soil microorganisms have also been isolated and proved efficient for the mineralization of PLLA on agar plates or in growing medium.486,487”
From Catalysis as an Enabling Science for Sustainable Polymers (2018) · cites this paper as [487]
Cites
Response Surface Methodology: Process and Product Optimization Using Designed Experiments
Gunst, R. F., Myers, R. H., & Montgomery, D. C. (1996) · Technometrics · doi:10.2307/1270613 · Cited by 9,576 · see citing works
On the Experimental Attainment of Optimum Conditions
Box, G. E. P., & Wilson, K. (1992) · Springer series in statistics · doi:10.1007/978-1-4612-4380-9_23 · Cited by 3,472 · see citing works
Degradation of Poly(L-lactide) by a Fungus
Jarerat, A., & Tokiwa, Y. (2001) · Macromolecular Bioscience · doi:10.1002/1616-5195(20010601)1:4 · Cited by 141 · see citing works
Singh, R. S., Sooch, B. S., & Puri, M. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2006.09.011 · Cited by 128 · see citing works
Poly(L‐lactide). IX. Hydrolysis in acid media
Tsuji, H., & Nakahara, K. (2002) · Journal of Applied Polymer Science · doi:10.1002/app.10813 · Cited by 124 · see citing works
Isolation of thermophiles degrading poly(l-lactic acid)
Tomita, K., Kuroki, Y., & Nagai, K. (1999) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(99)80148-0 · Cited by 99 · see citing works
Sakai, K., Kawano, H., Iwami, A., Nakamura, M., & Moriguchi, M. (2001) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(01)80266-8 · Cited by 97 · see citing works
Jarerat, A., Tokiwa, Y., & Tanaka, H. (2006) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0343-4 · Cited by 96 · see citing works
Optimization of thermostable alkaline protease production from species of Bacillus using rice bran
Krishna, S. B. N., & Kodidhela, L. D. (2005) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb2005.000-3132 · Cited by 94 · see citing works
Anbu, P., Gopinath, S. C. B., Hilda, A., Lakshmipriya, T., & Annadurai, G. (2006) · Bioresource Technology · doi:10.1016/j.biortech.2006.05.047 · Cited by 87 · see citing works
Sukkhum, S., Tokuyama, S., Tamura, T., & Kitpreechavanich, V. (2009) · The Journal of General and Applied Microbiology · doi:10.2323/jgam.55.459 · Cited by 71 · see citing works
Self-citationMicrobial Poly(L-Lactide)-Degrading Enzyme Induced by Amino Acids, Peptides, and Poly(L-Amino Acids)
Jarerat, A., Tokiwa, Y., & Tanaka, H. (2004) · Journal of Polymers and the Environment · doi:10.1023/b:jooe.0000038545.69235.f2 · Cited by 59 · see citing works
Degradation of poly(l-lactic acid) by a newly isolated thermophile
Tomita, K., Nakajima, T., Kikuchi, Y., & Miwa, N. (2004) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2003.12.006 · Cited by 58 · see citing works
Sukkhum, S., Tokuyama, S., & Kitpreechavanich, V. (2009) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-008-0207-0 · Cited by 58 · see citing works
Self-citationOptimization of protease production from newly isolated strain of Bacillus sp. PCSIR EA-3
Qader, S. A. U., Shireen, E., Iqbal, S., & Anwar, A. (2009) · Indian Journal of Biotechnology · Cited by 31 · see citing works
Poly( L ‐lactide)‐degrading activity of a newly isolated bacterium
Kim, M.-N., Kim, W. G., Weon, H., & Lee, S. H. (2008) · Journal of Applied Polymer Science · doi:10.1002/app.26658 · Cited by 26 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Catalysis as an Enabling Science for Sustainable Polymers
Zhang, X., Fevre, M., Jones, G. O., & Waymouth, R. M. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00329 · Cited by 936 · see citing works
Review on the current status of polymer degradation: a microbial approach
Pathak, V., & Navneet (2017) · Bioresources and Bioprocessing · doi:10.1186/s40643-017-0145-9 · Cited by 652 · see citing works
Sustainability of bioplastics: Opportunities and challenges
Thakur, S., Chaudhary, J., Sharma, B., Verma, A., Tamulevicius, S., & Thakur, V. K. (2018) · Current Opinion in Green and Sustainable Chemistry · doi:10.1016/j.cogsc.2018.04.013 · Cited by 345 · see citing works
Thermophilic and alkaliphilic Actinobacteria: biology and potential applications
Shivlata, L., & Satyanarayana, T. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.01014 · Cited by 294 · see citing works
Hydrolysis and Biodegradation of Poly(lactic acid)
Gorrasi, G., & Pantani, R. (2017) · Advances in polymer science · doi:10.1007/12_2016_12 · Cited by 192 · see citing works
“Up to date, only few reports give evidence of the ability of microorganisms or enzymes to cleave high molecular weight PLA directly [23,133,134] The ability of PLA degradation by several actinomycetes [135] and thermophilic PLAdegrading bacteria such as Bacillus licheniformis, Bacillus brevis, Bacillus stearothermophi…”
BackgroundActinobacteria as Promising Candidate for Polylactic Acid Type Bioplastic Degradation
Butbunchu, N., & ., W. P.-A. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02834 · Cited by 130 · see citing works
“A yield of 800 mg/L L-lactic acid was obtained from the degradation of 4,000 mg/L L-PLA powder at 60◦C for 8 h (Sukkhum et al., 2012).”
MethodSikorska, W., Musioł, M., Nowak, B., Pajak, J., Łabużek, S., Kowalczuk, M. M., & Adamus, G. (2015) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2015.03.021 · Cited by 76 · see citing works
Approaches in Sustainable, Biobased Multilayer Packaging Solutions
Eißenberger, K., Ballesteros, A., Bisschop, R. D., Bugnicourt, E., Cinelli, P., Defoin, M., Demeyer, E., Fürtauer, S., Gioia, C., Gómez, L., et al. (2023) · Polymers · doi:10.3390/polym15051184 · Cited by 73 · see citing works
Isolation and Characterization of Polyester-Based Plastics-Degrading Bacteria from Compost Soils
Sriyapai, P., Chansiri, K., & Sriyapai, T. (2018) · Microbiology · doi:10.1134/s0026261718020157 · Cited by 67 · see citing works
“Strain APL3 produced the highest PBS depolymerase at 45°C and pH 7.0 for 5 days with final concentration of 1% PBS. PBS was the best carbon source and the degradation of PBS emulsion by culture supernatant was 19.4 U/mL. Strain TP4 produced the highest PBSA depolymerase at 50°C and pH 7.0 for 5 days using 0.5% (w/v) P…”
InfluentialAugmentation of a Microbial Consortium for Enhanced Polylactide (PLA) Degradation
Nair, N. R., Sekhar, V. C., & Nampoothiri, K. M. (2015) · Indian Journal of Microbiology · doi:10.1007/s12088-015-0559-z · Cited by 54 · see citing works
Panyachanakul, T., Sorachart, B., Lumyong, S., Lorliam, W., Kitpreechavanich, V., & Krajangsang, S. (2019) · Electronic Journal of Biotechnology · doi:10.1016/j.ejbt.2019.04.005 · Cited by 38 · see citing works
“[20] reported that PLA was degraded by protease from A.”
BackgroundImportance of Actinobacteria for Bioremediation
Chaturvedi, S., & Khurana, S. M. P. (2019) · Springer eBooks · doi:10.1007/978-981-13-8499-8_13 · Cited by 29 · see citing works
Lomthong, T., Hanphakphoom, S., Yoksan, R., & Kitpreechavanich, V. (2015) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2015.07.011 · Cited by 29 · see citing works
Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization
Youngpreda, A., Panyachanakul, T., Kitpreechavanich, V., Sirisansaneeyakul, S., Suksamrarn, S., Tokuyama, S., & Krajangsang, S. (2016) · Journal of Polymers and the Environment · doi:10.1007/s10924-016-0885-1 · Cited by 28 · see citing works
Lomthong, T., Lertwattanasakul, N., & Kitpreechavanich, V. (2016) · Starch - Stärke · doi:10.1002/star.201600018 · Cited by 21 · see citing works
Faiza, W. F. N. W., Firouzi, A., Islam, M. R., Sumdani, M. G., & Taher, A. (2021) · Polymer Composites · doi:10.1002/pc.26007 · Cited by 19 · see citing works
Sheel, A., & Pant, D. (2017) · Energy, environment, and sustainability · doi:10.1007/978-981-10-7413-4_4 · Cited by 19 · see citing works
Timmins, R. L., Kumar, A., Röhrl, M., Havlíček, K., Agarwal, S., & Breu, J. (2021) · Macromolecular Materials and Engineering · doi:10.1002/mame.202100727 · Cited by 18 · see citing works
Penkhrue, W., Kanpiengjai, A., Khanongnuch, C., Masaki, K., ., W. P.-A., Punyodom, W., & Lumyong, S. (2017) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2017.1315597 · Cited by 17 · see citing works
“The production of the PLA-degrading enzyme from Actinomadura sp. T16-1 was also successfully enhanced by 3.36-fold after production by the 3-”
BackgroundInfluentialPanyachanakul, T., Kitpreechavanich, V., Tokuyama, S., & Krajangsang, S. (2017) · Electronic Journal of Biotechnology · doi:10.1016/j.ejbt.2017.09.001 · Cited by 17 · see citing works
“[11] reported that almost 800 mg/L lactic acid and 500 mg/L oligomers were obtained after an 8-h degradation of 4000 mg/L PDLLA powder by strain T16-1.”
MethodKhosrovyan, A., Melkonyan, H., Rshtuni, L., Gabrielyan, B. K., & Kahru, A. (2023) · Water · doi:10.3390/w15112133 · Cited by 15 · see citing works
Lomthong, T., Yoksan, R., Lumyong, S., & Kitpreechavanich, V. (2018) · Waste and Biomass Valorization · doi:10.1007/s12649-018-0519-z · Cited by 15 · see citing works
Lomthong, T., Hanphakphoom, S., Kongsaeree, P. T., Srisuk, N., Guicherd, M., Cioci, G., Duquesne, S., Marty, A., & Kitpreechavanich, V. (2017) · Polymer Degradation and Stability · doi:10.1016/j.polymdegradstab.2017.06.017 · Cited by 15 · see citing works
Li, F., Zhan, Y., Liu, D., Liu, D., Xia, H., & Chen, S. (2013) · Journal of Polymers and the Environment · doi:10.1007/s10924-013-0590-2 · Cited by 11 · see citing works
Krajangsang, S., Dechsresawut, N., Lomthong, T., & Samaimai, S. (2021) · 3 Biotech · doi:10.1007/s13205-021-03060-8 · Cited by 5 · see citing works
Sujarit, K., Pannim, B., Kuakkhunthod, N., Uywannang, U., Sakdapetsiri, C., Panyachanakul, T., Krajangsang, S., Phanngoen, S., Kitpreechavanich, V., & Lomthong, T. (2025) · 3 Biotech · doi:10.1007/s13205-025-04219-3 · Cited by 5 · see citing works
“For hydrolysis of PLA polymers, the pH of the reaction ranging from 8.0 to 10.0 was optimum for PLA degradation (Sukkhum et al. 2012; Lomthong et al. 2021).”
InfluentialOmay, D., & Güvenilir, Y. (2014) · International Polymer Processing · doi:10.3139/217.2835 · Cited by 4 · see citing works
Kumar, R., & Razdan, S. (2022) · doi:10.1002/9781119800897.ch10 · Cited by 2 · see citing works
Crystallization in poly(l-Lactic Acid) Foams
Longo, A., Maio, E. D., & Lorenzo, M. L. D. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95486-0.00077-6 · Cited by 1 · see citing works
Sakdapetsiri, C., Chumworathayee, W., Thongpoem, P., Seedam, R., Chotikawandee, N., Thimyaingam, C., Suwannachart, C., Busarakam, K., Ayudthaya, S. P. N., Kitpreechavanich, V., et al. (2026) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2026.104037 · Cited by 1 · see citing works
STUDY OF BIOLOGICAL RECYCLING PROCESS OF POLY (DL-LACTIC ACID) USING ACTINOMYCETES ENZYMES
Panyachanakul, T., ปัญญาชนะกุล, ฐ., Krajangsang, S., & กระจ่างสังข์, ส. (2019)
Falzarano, M., Magnosi, M., Polettini, A., Pomi, R., & Rossi, A. (2026) · Critical Reviews in Environmental Science and Technology · doi:10.1080/10643389.2026.2708126
- Authors
- Sukkhum, S.
- Affiliations
- Srinakharinwirot University (TH)
- Corresponding author
- Sukkhum, Sukhumaporn
- Venue
- Journal of Microbiology and Biotechnology
- Published
- 2012-01-28
- Volume
- 22
- Issue
- 1
- Pages
- 92-99
- ISSN
- 1017-7825 (ISSN-L)
- 1738-8872
- OpenAlex
- W2018072547
- Cited by
- 32
- References
- 16
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2019: 4 · 2018: 3 · 2017: 7 · 2016: 2 · 2015: 4 · 2014: 1 · 2013: 1
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- poly(L-lactic acid), airlift bioreactor, enzyme production optimization, air flow rate, pH, temperature, lactic acid production, biopolymer degradation
- MeSH terms
- Actinomycetales, Aerobiosis, Culture Media, Enzymes, Hydrogen-Ion Concentration, Polyesters, Temperature, Models, Statistical, Bioreactors
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